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Strain identifier

BacDive ID: 13244

Type strain: Yes

Species: Amycolatopsis albidiflava

Strain Designation: G495-11

Strain history: ATCC 53205 <-- Bristol-Myers Co.; G495-11.

NCBI tax ID(s): 102226 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 11948

BacDive-ID: 13244

DSM-Number: 44639

keywords: 16S sequence, mesophilic

description: Amycolatopsis albidiflava G495-11 is a mesophilic Actinobacterium that was isolated from soil.

NCBI tax id

  • NCBI tax id: 102226
  • Matching level: species

strain history

doi: 10.13145/bacdive13244.20221219.7.1

Name and taxonomic classification

@ref: 11948

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Pseudonocardiaceae

genus: Amycolatopsis

species: Amycolatopsis albidiflava

full scientific name: Amycolatopsis albidiflava Lee and Hah 2001

strain designation: G495-11

type strain: yes

Morphology

colony morphology

@refcolony colorincubation periodmedium used
19873Beige10-14 daysISP 2
19873Beige10-14 daysISP 3
19873Beige10-14 daysISP 4
19873Beige10-14 daysISP 5
19873Beige10-14 daysISP 6
19873Beige10-14 daysISP 7

multimedia

  • @ref: 11948
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_44639.jpg
  • caption: Medium 554 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11948N-Z-AMINE-MEDIUM (DSMZ Medium 554)yeshttps://mediadive.dsmz.de/medium/554Name: N-Z-AMINE-MEDIUM (DSMZ Medium 554) Composition: Starch 20.0 g/l Agar 20.0 g/l Glucose 10.0 g/l N-Z amine 5.0 g/l Yeast extract 5.0 g/l CaCO3 1.0 g/l Distilled water
11948GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19873ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19873ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19873ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19873ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19873ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19873ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

culture temp

@refgrowthtypetemperaturerange
11948positivegrowth28mesophilic
19873positiveoptimum28mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

observation

  • @ref: 67770
  • observation: quinones: MK-9(H4)

enzymes

@refvalueactivityec
68382alpha-fucosidase+3.2.1.51
68382alpha-mannosidase+3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin+3.4.21.1
68382trypsin+3.4.21.4
68382cystine arylamidase+3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)+
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
19873+++++++++++-+---+++

Isolation, sampling and environmental information

isolation

@refsample type
11948soil
67770Soil

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_7930.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_408;97_462;98_606;99_7930&stattab=map
  • Last taxonomy: Amycolatopsis
  • 16S sequence: AB327251
  • Sequence Identity:
  • Total samples: 101
  • soil counts: 59
  • animal counts: 14
  • plant counts: 28

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
119481Risk group (German classification)
198731Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Amycolatopsis albidoflavus 16S rRNA gene, strain IMSNU 22139TAJ2528321474ena102226
20218Amycolatopsis albidoflavus gene for 16S rRNA, partial sequence, strain: NBRC 100337AB3272511444ena102226

GC content

  • @ref: 67770
  • GC-content: 68.5
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 11948

culture collection no.: DSM 44639, ATCC 53205, IMSNU 22139, JCM 11300, KCTC 9471, NBRC 100337, NCIMB 14254, NRRL B-24149

straininfo link

@refpassport
20218http://www.straininfo.net/strains/301888
20218http://www.straininfo.net/strains/116504
20218http://www.straininfo.net/strains/116509
20218http://www.straininfo.net/strains/116507
20218http://www.straininfo.net/strains/116508

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny11321110Amycolatopsis albidoflavus sp. nov.Lee SD, Hah YCInt J Syst Evol Microbiol10.1099/00207713-51-2-6452001Actinomycetales/*classification/genetics, Bacterial Typing Techniques, DNA, Ribosomal/*genetics, Molecular Sequence Data, RNA, Ribosomal, 16S/*genetics, Terminology as TopicGenetics
Phylogeny16403887Reclassification of Amycolatopsis orientalis DSM 43387 as Amycolatopsis benzoatilytica sp. nov.Majumdar S, Prabhagaran SR, Shivaji S, Lal RInt J Syst Evol Microbiol10.1099/ijs.0.63766-02006Actinomycetales/chemistry/*classification/isolation & purification/physiology, Caseins/metabolism, Fatty Acids, Gelatin/metabolism, Humans, Hydrolysis, Hypoxanthine/metabolism, Lactose/metabolism, Lipids, Molecular Sequence Data, Mycetoma/*microbiology, Neck, Polysorbates/metabolism, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Homology, Nucleic Acid, Species Specificity, Xanthine/metabolismMetabolism
Phylogeny32250240Amycolatopsis nivea sp. nov., isolated from a Yellow River sample.Niu MM, Ming H, Cheng LJ, Zhao ZL, Ji WL, Li M, Yi BF, Xia TT, Nie GXInt J Syst Evol Microbiol10.1099/ijsem.0.0041342020Actinomycetales/*classification/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Glycolipids/chemistry, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Rivers/*microbiology, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry, Water MicrobiologyTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
11948Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44639)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44639
19873Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM44639.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta