Strain identifier
BacDive ID: 13244
Type strain: 
Species: Amycolatopsis albidoflava
Strain Designation: G495-11
Strain history: ATCC 53205 <-- Bristol-Myers Co.; G495-11.
NCBI tax ID(s): 102226 (species)
version 8.1 (current version)
General
@ref: 11948
BacDive-ID: 13244
DSM-Number: 44639
keywords: 16S sequence, Bacteria, mesophilic
description: Amycolatopsis albidoflava G495-11 is a mesophilic bacterium that was isolated from soil.
NCBI tax id
- NCBI tax id: 102226
- Matching level: species
strain history
| @ref | history |
|---|---|
| 11948 | <- T. Kudo, JCM <- ATCC <- Bristol-Myers Co.; G495-11 |
| 67770 | ATCC 53205 <-- Bristol-Myers Co.; G495-11. |
doi: 10.13145/bacdive13244.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Pseudonocardiales
- family: Pseudonocardiaceae
- genus: Amycolatopsis
- species: Amycolatopsis albidoflava
- full scientific name: Amycolatopsis albidoflava corrig. Lee and Hah 2001
synonyms
- @ref: 20215
- synonym: Amycolatopsis albidoflavus
@ref: 11948
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Pseudonocardiaceae
genus: Amycolatopsis
species: Amycolatopsis albidiflava
full scientific name: Amycolatopsis albidiflava Lee and Hah 2001
strain designation: G495-11
type strain: yes
Morphology
colony morphology
| @ref | colony color | incubation period | medium used |
|---|---|---|---|
| 19873 | Beige | 10-14 days | ISP 2 |
| 19873 | Beige | 10-14 days | ISP 3 |
| 19873 | Beige | 10-14 days | ISP 4 |
| 19873 | Beige | 10-14 days | ISP 5 |
| 19873 | Beige | 10-14 days | ISP 6 |
| 19873 | Beige | 10-14 days | ISP 7 |
multimedia
- @ref: 11948
- multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_44639.jpg
- caption: Medium 554 28°C
- intellectual property rights: © Leibniz-Institut DSMZ
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 11948 | N-Z-AMINE-MEDIUM (DSMZ Medium 554) | yes | https://mediadive.dsmz.de/medium/554 | Name: N-Z-AMINE-MEDIUM (DSMZ Medium 554) Composition: Starch 20.0 g/l Agar 20.0 g/l Glucose 10.0 g/l N-Z amine 5.0 g/l Yeast extract 5.0 g/l CaCO3 1.0 g/l Distilled water |
| 11948 | GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) | yes | https://mediadive.dsmz.de/medium/65 | Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water |
| 19873 | ISP 2 | yes | Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes | |
| 19873 | ISP 3 | yes | Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms: | |
| 19873 | ISP 4 | yes | Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml | |
| 19873 | ISP 5 | yes | Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes | |
| 19873 | ISP 6 | yes | Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes | |
| 19873 | ISP 7 | yes | Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes |
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 11948 | positive | growth | 28 | mesophilic |
| 19873 | positive | optimum | 28 | mesophilic |
| 67770 | positive | growth | 28 | mesophilic |
Physiology and metabolism
observation
- @ref: 67770
- observation: quinones: MK-9(H4)
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 68382 | alkaline phosphatase | + | 3.1.3.1 |
| 68382 | esterase (C 4) | + | |
| 68382 | esterase lipase (C 8) | + | |
| 68382 | lipase (C 14) | + | |
| 68382 | leucine arylamidase | + | 3.4.11.1 |
| 68382 | valine arylamidase | + | |
| 68382 | cystine arylamidase | + | 3.4.11.3 |
| 68382 | trypsin | + | 3.4.21.4 |
| 68382 | alpha-chymotrypsin | + | 3.4.21.1 |
| 68382 | acid phosphatase | + | 3.1.3.2 |
| 68382 | naphthol-AS-BI-phosphohydrolase | + | |
| 68382 | alpha-galactosidase | - | 3.2.1.22 |
| 68382 | beta-galactosidase | + | 3.2.1.23 |
| 68382 | beta-glucuronidase | - | 3.2.1.31 |
| 68382 | alpha-glucosidase | - | 3.2.1.20 |
| 68382 | beta-glucosidase | - | 3.2.1.21 |
| 68382 | N-acetyl-beta-glucosaminidase | + | 3.2.1.52 |
| 68382 | alpha-mannosidase | + | 3.2.1.24 |
| 68382 | alpha-fucosidase | + | 3.2.1.51 |
API zym
| @ref | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 19873 | + | + | + | + | + | + | + | + | + | + | + | - | + | - | - | - | + | + | + |
Isolation, sampling and environmental information
isolation
| @ref | sample type |
|---|---|
| 11948 | soil |
| 67770 | Soil |
isolation source categories
- Cat1: #Environmental
- Cat2: #Terrestrial
- Cat3: #Soil
taxonmaps
- @ref: 69479
- File name: preview.99_7930.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_408;97_462;98_606;99_7930&stattab=map
- Last taxonomy: Amycolatopsis
- 16S sequence: AB327251
- Sequence Identity:
- Total samples: 101
- soil counts: 59
- animal counts: 14
- plant counts: 28
Safety information
risk assessment
| @ref | biosafety level | biosafety level comment |
|---|---|---|
| 11948 | 1 | Risk group (German classification) |
| 19873 | 1 | Risk group (German classification) |
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 20218 | Amycolatopsis albidoflavus 16S rRNA gene, strain IMSNU 22139T | AJ252832 | 1474 | ena | 102226 |
| 20218 | Amycolatopsis albidoflavus gene for 16S rRNA, partial sequence, strain: NBRC 100337 | AB327251 | 1444 | ena | 102226 |
GC content
- @ref: 67770
- GC-content: 68.5
- method: high performance liquid chromatography (HPLC)
External links
@ref: 11948
culture collection no.: DSM 44639, ATCC 53205, IMSNU 22139, JCM 11300, KCTC 9471, NBRC 100337, NCIMB 14254, NRRL B-24149
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 11321110 | Amycolatopsis albidoflavus sp. nov. | Lee SD, Hah YC | Int J Syst Evol Microbiol | 10.1099/00207713-51-2-645 | 2001 | Actinomycetales/*classification/genetics, Bacterial Typing Techniques, DNA, Ribosomal/*genetics, Molecular Sequence Data, RNA, Ribosomal, 16S/*genetics, Terminology as Topic | Genetics |
| Phylogeny | 16403887 | Reclassification of Amycolatopsis orientalis DSM 43387 as Amycolatopsis benzoatilytica sp. nov. | Majumdar S, Prabhagaran SR, Shivaji S, Lal R | Int J Syst Evol Microbiol | 10.1099/ijs.0.63766-0 | 2006 | Actinomycetales/chemistry/*classification/isolation & purification/physiology, Caseins/metabolism, Fatty Acids, Gelatin/metabolism, Humans, Hydrolysis, Hypoxanthine/metabolism, Lactose/metabolism, Lipids, Molecular Sequence Data, Mycetoma/*microbiology, Neck, Polysorbates/metabolism, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Homology, Nucleic Acid, Species Specificity, Xanthine/metabolism | Metabolism |
| Phylogeny | 32250240 | Amycolatopsis nivea sp. nov., isolated from a Yellow River sample. | Niu MM, Ming H, Cheng LJ, Zhao ZL, Ji WL, Li M, Yi BF, Xia TT, Nie GX | Int J Syst Evol Microbiol | 10.1099/ijsem.0.004134 | 2020 | Actinomycetales/*classification/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Glycolipids/chemistry, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Rivers/*microbiology, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry, Water Microbiology | Transcriptome |
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 11948 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44639) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-44639 | |||
| 19873 | Wink, J. | http://www.dsmz.de/microorganisms/wink_pdf/DSM44639.pdf | Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
| 68382 | Automatically annotated from API zym | |||||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta |