Strain identifier

BacDive ID: 13241

Type strain: Yes

Species: Amycolatopsis balhimycina

Strain Designation: FH 1894

Strain history: CIP <- 2004, J. Wink, Aventis, Frankfurt am Main, Germany: strain FH 1894 <- Höchst India Ltd

NCBI tax ID(s): 1089545 (strain), 208443 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 11906

BacDive-ID: 13241

DSM-Number: 44591

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-positive, rod-shaped, antibiotic compound production

description: Amycolatopsis balhimycina FH 1894 is an obligate aerobe, mesophilic, Gram-positive bacterium that builds an aerial mycelium and produces antibiotic compounds.

NCBI tax id

NCBI tax idMatching level
208443species
1089545strain

strain history

@refhistory
11906<- J. Wink, Aventis; FH 1894 <- Höchst India Ltd.
67770DSM 44591 <-- J. Wink FH 1894 <-- Höchst India Ltd..
116716CIP <- 2004, J. Wink, Aventis, Frankfurt am Main, Germany: strain FH 1894 <- Höchst India Ltd

doi: 10.13145/bacdive13241.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Pseudonocardiales
  • family: Pseudonocardiaceae
  • genus: Amycolatopsis
  • species: Amycolatopsis balhimycina
  • full scientific name: Amycolatopsis balhimycina Wink et al. 2003

@ref: 11906

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Pseudonocardiaceae

genus: Amycolatopsis

species: Amycolatopsis balhimycina

full scientific name: Amycolatopsis balhimycina Wink et al. 2003 emend. Nouioui et al. 2018

strain designation: FH 1894

type strain: yes

Morphology

cell morphology

  • @ref: 116716
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

colony morphology

@refcolony colorincubation periodmedium used
20077Daffodil yellow (1007)10-14 daysISP 2
20077Daffodil yellow (1007)10-14 daysISP 3
20077Daffodil yellow (1007)10-14 daysISP 4
20077Daffodil yellow (1007)10-14 daysISP 5
20077Daffodil yellow (1007)10-14 daysISP 6
20077Daffodil yellow (1007)10-14 daysISP 7
116716

multicellular morphology

@refforms multicellular complexmedium namecomplex namecomplex color
20077noISP 2
20077yesISP 3Aerial MyceliumWhite
20077noISP 4
20077noISP 5
20077noISP 6
20077noISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11906GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
20077ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
20077ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
20077ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
20077ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
20077ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
20077ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
40012MEDIUM 57 - for Streptomyces, Nocardioides, Lentzea albidocapillata and Streptoverticillium reticulumyesDistilled water make up to (1000.000 ml);Agar (15.000 g);Glucose (4.000g);Yeast extract (4.000 g);Malt extract (10.000 g);Calcium carbonate (2.000 g)
116716CIP Medium 57yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=57

culture temp

@refgrowthtypetemperaturerange
11906positivegrowth28mesophilic
20077positiveoptimum28mesophilic
40012positivegrowth30mesophilic
67770positivegrowth28mesophilic
116716positivegrowth25-37mesophilic
116716nogrowth10psychrophilic
116716nogrowth41thermophilic
116716nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 116716
  • oxygen tolerance: obligate aerobe

compound production

@refcompound
11906balhimycin
20077Balhimycin

halophily

@refsaltgrowthtested relationconcentration
20077NaClpositivemaximum5 %
116716NaClpositivegrowth0 %
116716NaClnogrowth2 %
116716NaClnogrowth4 %
116716NaClnogrowth6 %
116716NaClnogrowth8 %
116716NaClnogrowth10 %

observation

  • @ref: 67770
  • observation: quinones: MK-9(H4)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2007717234glucose+
2007722599arabinose+
2007717992sucrose+
2007718222xylose-
2007717268myo-inositol+
2007729864mannitol+
2007728757fructose+
2007726546rhamnose+
2007716634raffinose+/-
2007762968cellulose+/-
683685291gelatin+hydrolysis
6836827897tryptophan-energy source
6836816199urea+hydrolysis
6836816947citrate+assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
6836829016arginine-hydrolysis
11671616947citrate-carbon source
1167164853esculin+hydrolysis
116716606565hippurate+hydrolysis
11671617632nitrate-reduction
11671616301nitrite-reduction
11671617632nitrate-respiration

antibiotic resistance

  • @ref: 116716
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

@refmetaboliteproductionChebi-ID
67770balhimycinyes
68368acetoinyes15688
68368indoleno35581
68368hydrogen sulfideno16136
116716indoleno35581

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole testmethylred-test
6836815688acetoin+
6836835581indole-
11671615688acetoin-
11671617234glucose-

enzymes

@refvalueactivityec
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease+3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase-3.2.1.23
116716oxidase-
116716beta-galactosidase+3.2.1.23
116716alcohol dehydrogenase-1.1.1.1
116716gelatinase-
116716amylase+
116716DNase-
116716caseinase-3.4.21.50
116716catalase+1.11.1.6
116716tween esterase+
116716gamma-glutamyltransferase+2.3.2.2
116716lecithinase-
116716lipase-
116716lysine decarboxylase-4.1.1.18
116716ornithine decarboxylase-4.1.1.17
116716tryptophan deaminase-
116716urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382leucine arylamidase+3.4.11.1
68382acid phosphatase+3.1.3.2
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
20077+++++++++++++-+++-+
116716+++-+----+--+-+-+---

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
20077----+-+--++

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
116716+++++--+-+---+----+-++-++-+----+++---------------+---------++-+++--------+-----++-----+--++-------+

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
11906soil sampleIndiaINDAsia
67770SoilIndiaINDAsia
116716Environment, Soil sampleIndiaINDAsia

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_660.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_408;97_462;98_532;99_660&stattab=map
  • Last taxonomy: Amycolatopsis
  • 16S sequence: AJ508239
  • Sequence Identity:
  • Total samples: 5860
  • soil counts: 4583
  • aquatic counts: 156
  • animal counts: 306
  • plant counts: 815

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
119061Risk group (German classification)
200771German classification
1167161Risk group (French classification)

Sequence information

16S sequences

  • @ref: 11906
  • description: Amycolatopsis balhimycetica 16S rRNA gene, type strain DSM 44591T
  • accession: AJ508239
  • length: 1449
  • database: ena
  • NCBI tax ID: 208443

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Amycolatopsis balhimycina FH 1894 strain DSM 445911089545.3wgspatric1089545
66792Amycolatopsis balhimycina FH 18942517434013draftimg1089545
67770Amycolatopsis balhimycina FH 1894 DSM 44591GCA_000384295scaffoldncbi1089545

GC content

  • @ref: 67770
  • GC-content: 70.8
  • method: genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno92.38no
flagellatedno97.49no
gram-positiveyes85.989no
anaerobicno99.364no
aerobicyes92.089no
halophileno91.969no
spore-formingyes88.993no
glucose-utilyes90.06no
thermophileno98.346no
glucose-fermentno91.156no

External links

@ref: 11906

culture collection no.: DSM 44591, JCM 12668, NRRL B-24207, CIP 108516

straininfo link

  • @ref: 82446
  • straininfo: 97665

literature

  • topic: Phylogeny
  • Pubmed-ID: 12747408
  • title: Three new antibiotic producing species of the genus Amycolatopsis, Amycolatopsis balhimycina sp. nov., A. tolypomycina sp. nov., A. vancoresmycina sp. nov., and description of Amycolatopsis keratiniphila subsp. keratiniphila subsp. nov. and A. keratiniphila subsp. nogabecina subsp. nov.
  • authors: Wink JM, Kroppenstedt RM, Ganguli BN, Nadkarni SR, Schumann P, Seibert G, Stackebrandt E
  • journal: Syst Appl Microbiol
  • DOI: 10.1078/072320203322337290
  • year: 2003
  • mesh: Actinomycetales/*classification/genetics/metabolism/ultrastructure, Anti-Bacterial Agents/*biosynthesis, Culture Media/chemistry, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Ribosomal, 16S/analysis/genetics, Soil Microbiology
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitle
11906Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44591)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44591
20077Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM44591.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
40012Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/6147
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
82446Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID97665.1StrainInfo: A central database for resolving microbial strain identifiers
116716Curators of the CIPCollection of Institut Pasteur (CIP 108516)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108516