Strain identifier

BacDive ID: 132404

Type strain: Yes

Species: Enterobacter bugandensis

Strain Designation: 247, 247BMC, EB-247

Strain history: <- T. Chakraborty, Justus-Liebig-Universität, Giessen, Germany; EB-247 <- Weill Bugando Univ. College of Health Scs., Mwanza, Tanzania

NCBI tax ID(s): 881260 (species)

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General

@ref: 24193

BacDive-ID: 132404

DSM-Number: 29888

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-negative, motile, rod-shaped, colony-forming

description: Enterobacter bugandensis 247 is a facultative anaerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from human blood of a neonate.

NCBI tax id

  • NCBI tax id: 881260
  • Matching level: species

strain history

  • @ref: 24193
  • history: <- T. Chakraborty, Justus-Liebig-Universität, Giessen, Germany; EB-247 <- Weill Bugando Univ. College of Health Scs., Mwanza, Tanzania

doi: 10.13145/bacdive132404.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Enterobacteriaceae
  • genus: Enterobacter
  • species: Enterobacter bugandensis
  • full scientific name: Enterobacter bugandensis Doijad et al. 2016

@ref: 24193

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacteriales, not validated

family: Enterobacteriaceae

genus: Enterobacter

species: Enterobacter bugandensis

full scientific name: Enterobacter bugandensis Doijad et al. 2016

strain designation: 247, 247BMC, EB-247

type strain: yes

Morphology

cell morphology

  • @ref: 43786
  • gram stain: negative
  • cell length: 2-3 µm
  • cell width: 0.5 µm
  • cell shape: rod-shaped
  • motility: yes

colony morphology

@refcolony colorcolony shapeincubation periodmedium usedcolony size
43786Whitecircular1 dayLuria-Bertani agar
43786White, opaquecircular1 dayMacConkey agar1-2 mm

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
43786LB (Luria-Bertani) MEDIUMyes
43786MacConkey agaryes
24193BHI MEDIUM (DSMZ Medium 215)yeshttps://mediadive.dsmz.de/medium/215Name: BHI MEDIUM (DSMZ Medium 215) Composition: Brain heart infusion 37.0 g/l Distilled water
24193NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
43786positiveoptimum37mesophilic
24193positivegrowth30mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 43786
  • oxygen tolerance: facultative anaerobe

halophily

  • @ref: 43786
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-9 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4378643943methyl alpha-D-mannoside-assimilation
4378632032potassium gluconate-assimilation
4378627897tryptophan-energy source
4378627613amygdalin-fermentation
4378616947citrate+carbon source
4378617634D-glucose+fermentation
4378616899D-mannitol+fermentation
4378617924D-sorbitol+fermentation
4378617268myo-inositol+fermentation
4378630849L-arabinose+fermentation
4378662345L-rhamnose+fermentation
4378628053melibiose+fermentation
4378617992sucrose+fermentation
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose+builds acid from
6837132528turanose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837117716lactose+builds acid from
6837117306maltose+builds acid from
6837117057cellobiose+builds acid from
6837117814salicin+builds acid from
683714853esculin+builds acid from
6837118305arbutin+builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine-builds acid from
68371320061methyl alpha-D-glucopyranoside+builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol+builds acid from
6837117268myo-inositol+builds acid from
6837116813galactitol+builds acid from
6837112936D-galactose+builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose+builds acid from
6837116988D-ribose+builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol+builds acid from
6836817632nitrate-reduction
6836830849L-arabinose+fermentation
6836827613amygdalin+fermentation
6836828053melibiose+fermentation
6836862345L-rhamnose+fermentation
6836830911sorbitol+fermentation
6836816899D-mannitol+fermentation
6836817634D-glucose+fermentation
683685291gelatin-hydrolysis
6836827897tryptophan-energy source
6836816199urea-hydrolysis
6836816947citrate+assimilation
6836818257ornithine+degradation
6836825094lysine-degradation
6836829016arginine+hydrolysis

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitiveis resistantgroup ID
43786471744imipenemyesyes
4378643968meropenemyesyes
4378671415nitrofurantoinyesyes
4378628971ampicillinyesyes
43786amoxicillinyesyes10
4378648947clavulanic acidyesyes10
437868232piperacillinyesyes
43786piperacillin + tazobactamyesyes
43786124991cefalotinyesyes
437863515cefuroximeyesyes
43786209807cefoxitinyesyes
43786204928cefotaximeyesyes
437863508ceftazidimeyesyes
4378617833gentamicinyesyes
4378628864tobramycinyesyes
43786100241ciprofloxacinyesyes
43786100246norfloxacinyesyes
4378627902tetracyclineyesyes
4378645924trimethoprimyesyes26
437869332sulfamethoxazoleyesyes26
437863504cefpodoximeyesyes

metabolite production

@refChebi-IDmetaboliteproduction
4378615688acetoinno
4378616136hydrogen sulfideno
4378635581indoleno
6836817997dinitrogenyes
6836816301nitriteno
6836815688acetoinyes
6836835581indoleno
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
4378615688acetoin-
4378635581indole-
6836815688acetoin+
6836835581indole-

enzymes

@refvalueactivityec
43786catalase+1.11.1.6
43786cytochrome oxidase-1.9.3.1
43786beta-galactosidase+3.2.1.23
43786arginine dihydrolase+3.5.3.6
43786ornithine decarboxylase+4.1.1.17
43786urease-3.5.1.5
43786gelatinase-
43786tryptophan deaminase-4.1.99.1
43786lysine decarboxylase-4.1.1.18
68368cytochrome oxidase-1.9.3.1
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase+4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase+3.2.1.23

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOXNO2N2
24193++-++----+-+++/-+++/-+++--+
24193++-++----+-+++/-++++++
24193++-++----+-++-++++++--+

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
24193-+--+/-++---++/-+/-+/--+++++-+--++++++++/-+/---+---+-+--------
24193-+--+/-++---+----+/-+++/-+-+--+++++++/-----+/------+--------
24193-+--+++---+++++/-+++++-+--+++++++/-+/-+/---+---+/--+--------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
24193human blood of a neonateMwanzaTanzaniaTZAAfrica
43786A blood sample of neonate in MwanzaMwanzaTanzaniaTZAAfrica

isolation source categories

Cat1Cat2Cat3
#Host#Human#Child
#Host Body Product#Fluids#Blood

taxonmaps

  • @ref: 69479
  • File name: preview.99_120.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_6;96_7;97_7;98_17;99_120&stattab=map
  • Last taxonomy: Enterobacteriaceae
  • 16S sequence: HQ122932
  • Sequence Identity:
  • Total samples: 13334
  • soil counts: 615
  • aquatic counts: 1353
  • animal counts: 10132
  • plant counts: 1234

Sequence information

16S sequences

  • @ref: 24193
  • description: Enterobacter bugandensis strain 247BMC 16S ribosomal RNA gene, partial sequence
  • accession: HQ122932
  • length: 1444
  • database: ena
  • NCBI tax ID: 881260

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Enterobacter bugandensis EB-247GCA_900324475completencbi881260
66792Enterobacter bugandensis strain FDAARGOS 1427881260.84completepatric881260
66792Enterobacter bugandensis EB-2472791355295draftimg881260
66792Enterobacter bugandensis EB-2472859686987completeimg881260

GC content

@refGC-contentmethod
4378656genome sequence analysis
2419356sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno60.602no
gram-positiveno97.839yes
anaerobicno96.046yes
aerobicyes81.461yes
halophileno87.896no
spore-formingno93.289no
thermophileno99.535yes
glucose-utilyes95.469no
motileyes90.398no
glucose-fermentyes93.564yes

External links

@ref: 24193

culture collection no.: DSM 29888, NCCB 100573

straininfo link

  • @ref: 90745
  • straininfo: 401827

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny26637818Enterobacter bugandensis sp. nov., isolated from neonatal blood.Doijad S, Imirzalioglu C, Yao Y, Pati NB, Falgenhauer L, Hain T, Foesel BU, Abt B, Overmann J, Mirambo MM, Mshana SE, Chakraborty TInt J Syst Evol Microbiol10.1099/ijsem.0.0008212015
Metabolism29599516Enterobacter bugandensis: a novel enterobacterial species associated with severe clinical infection.Pati NB, Doijad SP, Schultze T, Mannala GK, Yao Y, Jaiswal S, Ryan D, Suar M, Gwozdzinski K, Bunk B, Mraheil MA, Marahiel MA, Hegemann JD, Sproer C, Goesmann A, Falgenhauer L, Hain T, Imirzalioglu C, Mshana SE, Overmann J, Chakraborty TSci Rep10.1038/s41598-018-23069-z2018Animals, Anti-Bacterial Agents/pharmacology, Cytokines/metabolism, Disease Models, Animal, Drug Resistance, Bacterial/drug effects, Enterobacter/*genetics/physiology, Enterobacteriaceae Infections/microbiology/mortality/*pathology, *Genome, Bacterial, Humans, Mice, O Antigens/chemistry/immunology, Plasmids/genetics/metabolism, Survival Rate, Transcriptome, Virulence/genetics, beta-Lactams/metabolismPathogenicity
Genetics30466389Multi-drug resistant Enterobacter bugandensis species isolated from the International Space Station and comparative genomic analyses with human pathogenic strains.Singh NK, Bezdan D, Checinska Sielaff A, Wheeler K, Mason CE, Venkateswaran KBMC Microbiol10.1186/s12866-018-1325-22018Anti-Bacterial Agents/pharmacology, *Drug Resistance, Multiple, Bacterial, Enterobacter/classification/*drug effects/*genetics/isolation & purification, Enterobacteriaceae Infections/*microbiology, Genome, Bacterial, Genomics, Humans, Microbial Sensitivity Tests, Multilocus Sequence Typing, Phylogeny, *Spacecraft/statistics & numerical data, Whole Genome SequencingPathogenicity
Phylogeny34037857Generation of electrical energy in a microbial fuel cell coupling acetate oxidation to Fe(3+) reduction and isolation of the involved bacteria.Becerril-Varela K, Serment-Guerrero JH, Manzanares-Leal GL, Ramirez-Duran N, Guerrero-Barajas CWorld J Microbiol Biotechnol10.1007/s11274-021-03077-42021Acetates/metabolism, Bacteria/*classification/genetics/isolation & purification, Biodegradation, Environmental, Bioelectric Energy Sources/*microbiology, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Iron/*chemistry, Oxidation-Reduction, Polymorphism, Restriction Fragment Length, RNA, Ribosomal, 16S/genetics, Sequence Analysis, RNA/*methods, Sewage/microbiologyTranscriptome

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
24193Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-29888Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 29888)
43786Swapnil Doijad, Can Imirzalioglu, Yancheng Yao, Niladri Bhusan Pati, Linda Falgenhauer, Torsten Hain, Bärbel U. Foesel, Birte Abt, Jö¨rg Overmann, Mariam M. Mirambo, Stephen E. Mshana, Trinad ChakrabortyEnterobacter bugandensis sp. nov., isolated from neonatal blood10.1099/ijsem.0.000821IJSEM 66: 968-974 201626637818
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68368Automatically annotated from API 20E
68371Automatically annotated from API 50CH acid
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
90745Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID401827.1