Strain identifier
BacDive ID: 132388
Type strain:
Species: Clostridium liquoris
Strain Designation: BEY10
Strain history: <- Y. Tao, CAS, Chengdu Inst. of Biology; BEY10 <- D. X. Li {2012}
NCBI tax ID(s): 1289519 (species)
General
@ref: 24177
BacDive-ID: 132388
DSM-Number: 100320
keywords: genome sequence, 16S sequence, Bacteria, obligate anaerobe, spore-forming, Gram-positive, rod-shaped, colony-forming
description: Clostridium liquoris BEY10 is an obligate anaerobe, spore-forming, Gram-positive bacterium that forms circular colonies and was isolated from fermentation pit used for the production of Chinese strong-flavoured liquor.
NCBI tax id
- NCBI tax id: 1289519
- Matching level: species
strain history
- @ref: 24177
- history: <- Y. Tao, CAS, Chengdu Inst. of Biology; BEY10 <- D. X. Li {2012}
doi: 10.13145/bacdive132388.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Clostridia
- order: Eubacteriales
- family: Clostridiaceae
- genus: Clostridium
- species: Clostridium liquoris
- full scientific name: Clostridium liquoris Yin et al. 2016
@ref: 24177
domain: Bacteria
phylum: Firmicutes
class: Clostridia
order: Anaeroplasmatales
family: Clostridiaceae
genus: Clostridium
species: Clostridium liquoris
full scientific name: Clostridium liquoris Yin et al. 2016
strain designation: BEY10
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell length | cell width | cell shape | motility | confidence |
---|---|---|---|---|---|---|
43778 | positive | 2.5-6 µm | 0.5-1 µm | rod-shaped | no | |
69480 | positive | 100 |
colony morphology
- @ref: 43778
- colony size: 1 mm
- colony color: Light yellow
- colony shape: circular
- incubation period: 3 days
- medium used: Peptone/yeast medium
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
43778 | Peptone/yeast medium | yes | ||
24177 | CHOPPED MEAT MEDIUM WITH CARBOHYDRATES (DSMZ Medium 110) | yes | https://mediadive.dsmz.de/medium/110 | Name: CHOPPED MEAT MEDIUM WITH CARBOHYDRATES (DSMZ Medium 110) Composition: Ground beef 500.0 g/l Casitone 30.0 g/l Agar 15.0 g/l K2HPO4 5.0 g/l Yeast extract 5.0 g/l D-Glucose 4.0 g/l Starch 1.0 g/l Maltose 1.0 g/l Cellobiose 1.0 g/l L-Cysteine HCl 0.5 g/l Ethanol 0.19 g/l Vitamin K3 0.05 g/l Hemin 0.005 g/l Sodium resazurin 0.0005 g/l Vitamin K1 NaOH Distilled water |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
43778 | positive | growth | 22-47 | |
43778 | positive | optimum | 37 | mesophilic |
24177 | positive | growth | 37 | mesophilic |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
43778 | positive | growth | 5.5-9 | alkaliphile |
43778 | positive | optimum | 7.5-8.5 |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance | confidence |
---|---|---|
43778 | obligate anaerobe | |
69480 | anaerobe | 99.999 |
spore formation
@ref | type of spore | spore formation | confidence |
---|---|---|---|
43778 | spore | yes | |
69481 | yes | 100 | |
69480 | yes | 100 |
halophily
@ref | salt | growth | tested relation | concentration | halophily level |
---|---|---|---|---|---|
43778 | NaCl | positive | growth | 0-4 %(w/v) | |
43778 | NaCl | positive | optimum | 0 %(w/v) | non-halophilic |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
43778 | 4853 | esculin | - | hydrolysis |
43778 | 5291 | gelatin | - | hydrolysis |
43778 | 17057 | cellobiose | + | carbon source |
43778 | 17234 | glucose | + | carbon source |
43778 | 17754 | glycerol | + | carbon source |
43778 | 17716 | lactose | + | carbon source |
43778 | maltose hydrate | + | carbon source | |
43778 | 29864 | mannitol | + | carbon source |
43778 | 27082 | trehalose | + | carbon source |
43778 | 18222 | xylose | + | carbon source |
43778 | 37684 | mannose | +/- | carbon source |
43778 | 6731 | melezitose | +/- | carbon source |
43778 | 16634 | raffinose | +/- | carbon source |
43778 | 26546 | rhamnose | +/- | carbon source |
43778 | 30911 | sorbitol | +/- | carbon source |
metabolite production
- @ref: 43778
- Chebi-ID: 35581
- metabolite: indole
- production: no
enzymes
- @ref: 43778
- value: catalase
- activity: -
- ec: 1.11.1.6
fatty acid profile
fatty acids
@ref fatty acid percentage 43778 C15:0 anteiso 31.9 43778 C15:1 anteiso A 4.6 43778 C17:0 anteiso 13.5 43778 C16:0 1.8 43778 C17:0W-cyclohexyl 16.2 43778 C14:0 iso 1.3 43778 C15:0 iso 4.5 43778 C16:0 iso 21.2 43778 C17:0 iso 1.3 43778 C18:0 iso 1.2 - type of FA analysis: whole cell analysis
- incubation medium: PYG
- agar/liquid: liquid
- incubation temperature: 37
- incubation_oxygen: anaerobic
- software version: Sherlock 6.1
- library/peak naming table: RTSBA6 6.10
- system: MIS MIDI
- cutoff value:
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent |
---|---|---|---|---|---|
24177 | fermentation pit used for the production of Chinese strong-flavoured liquor | Sichuan, Mianzhu | China | CHN | Asia |
43778 | An old fermentation pit | Mianzhu, Sichuan | China | CHN | Asia |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Engineered | #Food production | #Fermented |
#Engineered | #Other | |
#Engineered | #Food production | |
#Engineered | #Food production | #Beverage |
Safety information
risk assessment
- @ref: 24177
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 24177
- description: Clostridium liquoris strain BEY10 16S ribosomal RNA gene, partial sequence
- accession: KC331197
- length: 1474
- database: ena
- NCBI tax ID: 1289519
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Clostridium liquoris DSM 100320 | GCA_002995785 | contig | ncbi | 1289519 |
66792 | Clostridium liquoris strain DSM 100320 | 1289519.3 | wgs | patric | 1289519 |
66792 | Clostridium liquoris DSM 100320 | 2791355327 | draft | img | 1289519 |
GC content
@ref | GC-content | method |
---|---|---|
24177 | 34.4 | high performance liquid chromatography (HPLC) |
24177 | 31.0 | sequence analysis |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | yes | 100 | no |
motile | yes | 68.823 | yes |
gram-positive | yes | 59.569 | no |
anaerobic | yes | 99.449 | yes |
aerobic | no | 97.457 | no |
halophile | no | 80.034 | no |
spore-forming | yes | 95.493 | yes |
glucose-util | yes | 89.478 | no |
flagellated | no | 70.086 | no |
thermophile | no | 80.925 | yes |
glucose-ferment | no | 53.912 | no |
External links
@ref: 24177
culture collection no.: DSM 100320, ACCC 00785
straininfo link
- @ref: 90731
- straininfo: 402834
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | topic2 |
---|---|---|---|---|---|---|---|
Phylogeny | 26597830 | Clostridium liquoris sp. nov., isolated from a fermentation pit used for the production of Chinese strong-flavoured liquor. | Yin Q, Tao Y, Zhu X, Zhou Y, He X, Cheng L, Huang Y, Li D | Int J Syst Evol Microbiol | 10.1099/ijsem.0.000787 | 2015 | Biotechnology |
Genetics | 29724844 | First Insight into the Genome Sequence of Clostridium liquoris DSM 100320, a Butyrate- and Ethanol-Producing Bacterium. | Poehlein A, Neubauer H, Niemeyer P, Daniel R | Genome Announc | 10.1128/genomeA.00376-18 | 2018 |
Reference
@id | authors | title | doi/url | catalogue | journal | pubmed |
---|---|---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |||
24177 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-100320 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 100320) | |||
43778 | Qi Yin, Yong Tao, Xiaoye Zhu, Yan Zhou, Xiaohong He, Lei Cheng, Yan Huang, Daping Li | Clostridium liquoris sp. nov., isolated from a fermentation pit used for the production of Chinese strong-flavoured liquor | 10.1099/ijsem.0.000787 | IJSEM 66: 749-754 2016 | 26597830 | |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |||
66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | 10.1093/nar/gkaa1025 | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | |
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | Predictions based on the model GenomeNet Sporulation v. 1 | 10.21203/rs.3.rs-2527258/v1 | |||
90731 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID402834.1 |