Strain identifier

BacDive ID: 132363

Type strain: Yes

Species: Paenibacillus terreus

Strain Designation: D33

Strain history: <- Z. Huang, Nanjing Agric. Univ., College of Life Science, China; D33

NCBI tax ID(s): 1387834 (species)

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General

@ref: 24152

BacDive-ID: 132363

DSM-Number: 100035

keywords: 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive, motile, rod-shaped, colony-forming

description: Paenibacillus terreus D33 is an aerobe, spore-forming, mesophilic bacterium that forms irregular colonies and was isolated from forest soil.

NCBI tax id

  • NCBI tax id: 1387834
  • Matching level: species

strain history

  • @ref: 24152
  • history: <- Z. Huang, Nanjing Agric. Univ., College of Life Science, China; D33

doi: 10.13145/bacdive132363.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Paenibacillaceae
  • genus: Paenibacillus
  • species: Paenibacillus terreus
  • full scientific name: Paenibacillus terreus Huang et al. 2016

@ref: 24152

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Paenibacillaceae

genus: Paenibacillus

species: Paenibacillus terreus

full scientific name: Paenibacillus terreus Huang et al. 2016

strain designation: D33

type strain: yes

Morphology

cell morphology

  • @ref: 43996
  • gram stain: positive
  • cell length: 3.6-4.5 µm
  • cell width: 0.8-0.9 µm
  • cell shape: rod-shaped
  • motility: yes
  • flagellum arrangement: peritrichous

colony morphology

  • @ref: 43996
  • colony color: white
  • colony shape: irregular
  • medium used: Trypticase soy agar

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
43996Trypticase soy agaryes
24152CASO AGAR (MERCK 105458) (DSMZ Medium 220)yeshttps://mediadive.dsmz.de/medium/220Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
43996positiveoptimum30mesophilic
24152positivegrowth30mesophilic

culture pH

  • @ref: 43996
  • ability: positive
  • type: optimum
  • pH: 8

Physiology and metabolism

oxygen tolerance

  • @ref: 43996
  • oxygen tolerance: aerobe

spore formation

  • @ref: 43996
  • spore formation: yes

halophily

  • @ref: 43996
  • salt: NaCl
  • growth: positive
  • tested relation: optimum
  • concentration: 1 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4399617128adipate-assimilation
4399627689decanoate-assimilation
4399625115malate-assimilation
4399618401phenylacetate-assimilation
4399653258sodium citrate-assimilation
4399617108D-arabinose-builds acid from
4399618287L-fucose-builds acid from
43996168082-dehydro-D-gluconate-builds acid from
43996581435-dehydro-D-gluconate-builds acid from
4399615963ribitol-builds acid from
4399618333D-arabitol-builds acid from
4399628847D-fucose-builds acid from
4399662318D-lyxose-builds acid from
4399617924D-sorbitol-builds acid from
4399616443D-tagatose-builds acid from
4399616813galactitol-builds acid from
4399617113erythritol-builds acid from
4399618403L-arabitol-builds acid from
4399662345L-rhamnose-builds acid from
4399617266L-sorbose-builds acid from
4399665328L-xylose-builds acid from
439966731melezitose-builds acid from
43996320061methyl alpha-D-glucopyranoside-builds acid from
4399643943methyl alpha-D-mannoside-builds acid from
4399632032potassium gluconate-builds acid from
4399632528turanose-builds acid from
4399617151xylitol-builds acid from
4399617368hypoxanthine-hydrolysis
4399653426tween 80-hydrolysis
4399615318xanthine-hydrolysis
4399616947citrate+assimilation
4399617634D-glucose+assimilation
4399616899D-mannitol+assimilation
4399616024D-mannose+assimilation
4399630849L-arabinose+assimilation
4399617306maltose+assimilation
43996506227N-acetylglucosamine+assimilation
4399632032potassium gluconate+assimilation
4399612936D-galactose+builds acid from
4399665327D-xylose+builds acid from
4399630849L-arabinose+builds acid from
4399627613amygdalin+builds acid from
4399618305arbutin+builds acid from
4399617057cellobiose+builds acid from
4399615824D-fructose+builds acid from
4399617634D-glucose+builds acid from
4399616899D-mannitol+builds acid from
4399616024D-mannose+builds acid from
4399616988D-ribose+builds acid from
43996esculin ferric citrate+builds acid from
4399628066gentiobiose+builds acid from
4399615978glycerol+builds acid from
4399628087glycogen+builds acid from
4399617268myo-inositol+builds acid from
4399615443inulin+builds acid from
4399617716lactose+builds acid from
4399617306maltose+builds acid from
4399628053melibiose+builds acid from
4399674863methyl beta-D-xylopyranoside+builds acid from
43996506227N-acetylglucosamine+builds acid from
4399616634raffinose+builds acid from
4399617814salicin+builds acid from
4399628017starch+builds acid from
4399617992sucrose+builds acid from
4399627082trehalose+builds acid from
439965291gelatin+hydrolysis
4399628017starch+hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
4399630854indole-3-acetateno
4399616136hydrogen sulfideyes

metabolite tests

  • @ref: 43996
  • Chebi-ID: 5291
  • metabolite: gelatin
  • voges-proskauer-test: -
  • methylred-test: +

enzymes

@refvalueactivityec
43996catalase+1.11.1.6
43996cytochrome oxidase-1.9.3.1
43996urease-3.5.1.5
43996arginine dihydrolase-3.5.3.6
43996acid phosphatase+3.1.3.2
43996esterase (C 4)+
43996esterase Lipase (C 8)+
43996alpha-galactosidase+3.2.1.22
43996beta-galactosidase+3.2.1.23
43996beta-glucosidase+3.2.1.21
43996leucine arylamidase+3.4.11.1
43996naphthol-AS-BI-phosphohydrolase+
43996N-acetyl-beta-glucosaminidase-3.2.1.52
43996alkaline phosphatase-3.1.3.1
43996alpha-chymotrypsin-3.4.21.1
43996cystine arylamidase-3.4.11.3
43996alpha-fucosidase-3.2.1.51
43996alpha-glucosidase-3.2.1.20
43996beta-glucuronidase-3.2.1.31
43996lipase-
43996alpha-mannosidase-3.2.1.24
43996trypsin-3.4.21.4
43996valine arylamidase-

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    43996C15:0 anteiso49.5
    43996C17:0 anteiso15.8
    43996C16:010.1
    43996C16:1ω11c1.7
    43996C17:02.4
    43996C18:1ω11c2.4
    43996C15:0 iso2.8
    43996C16:0 iso9.3
    43996C17:0 iso2.9
  • type of FA analysis: whole cell analysis
  • incubation medium: TSA
  • incubation temperature: 30
  • incubation time: 48
  • library/peak naming table: TSBA6
  • system: MIS MIDI
  • instrument: model 6890;Hewlett Packard
  • method/protocol: Sasser, 1990
  • cutoff value:

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitude
24152forest soilJiangsu Province, Nanjing (31° 47' N, 118° 45' E)ChinaCHNAsia31.7833118.75
43996forest soilNanjing, Jiangsu ProvinceChinaCHNAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Forest
#Environmental#Terrestrial#Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_59649.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15358;96_18123;97_26514;98_33717;99_59649&stattab=map
  • Last taxonomy: Paenibacillus terreus
  • 16S sequence: KF479658
  • Sequence Identity:
  • Total samples: 9
  • soil counts: 4
  • aquatic counts: 1
  • animal counts: 1
  • plant counts: 3

Safety information

risk assessment

  • @ref: 24152
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 24152
  • description: Paenibacillus terreus 16S ribosomal RNA gene, partial sequence
  • accession: KF479658
  • length: 1516
  • database: ena
  • NCBI tax ID: 1387834

GC content

@refGC-contentmethod
4399651.1
2415251.1high performance liquid chromatography (HPLC)

External links

@ref: 24152

culture collection no.: DSM 100035, CCTCC AB 2015273, KACC 18491

straininfo link

  • @ref: 90709
  • straininfo: 407910

literature

  • topic: Phylogeny
  • Pubmed-ID: 26493172
  • title: Paenibacillus terreus sp. nov., isolated from forest soil.
  • authors: Huang Z, Dai W, Zhou Z, Wang G, Lin G, Yan X, Zhao F
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.000704
  • year: 2015
  • mesh: Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, *Forests, Molecular Sequence Data, Nucleic Acid Hybridization, Paenibacillus/*classification/genetics/isolation & purification, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Genetics

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
24152Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-100035Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 100035)
43996Zhi Huang, Wenjuan Dai, Zhijun Zhou, Guoxiang Wang, Guoqing Lin, Xixue Yan, Fei ZhaoPaenibacillus terreus sp. nov., isolated from forest soil10.1099/ijsem.0.000704IJSEM 66: 243-247 201626493172
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
90709Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID407910.1