Strain identifier

BacDive ID: 132362

Type strain: Yes

Species: Companilactobacillus insicii

Strain Designation: TMW1.2011

Strain history: <- M. Ehrmann, TU Munich, Freising-Weihenstephan, Germany; TMW1.2011

NCBI tax ID(s): 1732567 (species)

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General

@ref: 24151

BacDive-ID: 132362

DSM-Number: 29801

keywords: genome sequence, 16S sequence, Bacteria, facultative aerobe, Gram-positive, rod-shaped, colony-forming

description: Companilactobacillus insicii TMW1.2011 is a facultative aerobe, Gram-positive, rod-shaped bacterium that forms circular colonies and was isolated from fermented raw meat .

NCBI tax id

  • NCBI tax id: 1732567
  • Matching level: species

strain history

  • @ref: 24151
  • history: <- M. Ehrmann, TU Munich, Freising-Weihenstephan, Germany; TMW1.2011

doi: 10.13145/bacdive132362.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Companilactobacillus
  • species: Companilactobacillus insicii
  • full scientific name: Companilactobacillus insicii (Ehrmann et al. 2016) Zheng et al. 2020

  • synonyms

    • @ref: 20215
    • synonym: Lactobacillus insicii

@ref: 24151

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Companilactobacillus

species: Companilactobacillus insicii

full scientific name: Companilactobacillus insicii (Ehrmann et al. 2016) Zheng et al. 2020

strain designation: TMW1.2011

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
44054positive3-6 µm1 µmrod-shapedno
69480positive100
69480no97.139

colony morphology

  • @ref: 44054
  • colony size: 1 mm
  • colony color: beige
  • colony shape: circular
  • incubation period: 3 days
  • medium used: MRS agar

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
44054MRS agaryes
44054MRS brothyes
44054ROGOSAyes
24151MRS MEDIUM (DSMZ Medium 11)yeshttps://mediadive.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
44054positivegrowth8-45
44054positiveoptimum30mesophilic
24151positivegrowth30mesophilic

culture pH

  • @ref: 44054
  • ability: positive
  • type: growth
  • pH: 3.9-8.0
  • PH range: acidophile

Physiology and metabolism

oxygen tolerance

  • @ref: 44054
  • oxygen tolerance: facultative aerobe

spore formation

@reftype of sporespore formationconfidence
44054sporeno
69481no100
69480no99.999

halophily

  • @ref: 44054
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-10 %(w/v)

murein

  • @ref: 24151
  • murein short key: A11.42
  • type: A4alpha L-Lys-Gly-D-Asp

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
44054168082-dehydro-D-gluconate-builds acid from
4405427613amygdalin-builds acid from
4405417057cellobiose-builds acid from
4405415963ribitol-builds acid from
4405417108D-arabinose-builds acid from
4405428847D-fucose-builds acid from
4405412936D-galactose-builds acid from
4405417716lactose-builds acid from
4405462318D-lyxose-builds acid from
4405416899D-mannitol-builds acid from
440546731melezitose-builds acid from
4405463150D-rhamnose-builds acid from
4405417924D-sorbitol-builds acid from
4405416443D-tagatose-builds acid from
4405465327D-xylose-builds acid from
4405416813galactitol-builds acid from
4405417113erythritol-builds acid from
4405428066gentiobiose-builds acid from
4405424265gluconate-builds acid from
4405417754glycerol-builds acid from
4405428087glycogen-builds acid from
4405417268myo-inositol-builds acid from
4405415443inulin-builds acid from
4405430849L-arabinose-builds acid from
4405418403L-arabitol-builds acid from
4405418287L-fucose-builds acid from
4405462345L-rhamnose-builds acid from
4405417266L-sorbose-builds acid from
4405465328L-xylose-builds acid from
4405428053melibiose-builds acid from
4405437657methyl D-glucoside-builds acid from
4405428017starch-builds acid from
4405417992sucrose-builds acid from
4405427082trehalose-builds acid from
4405417151xylitol-builds acid from
4405415824D-fructose+builds acid from
4405417634D-glucose+builds acid from
4405416024D-mannose+builds acid from
4405417306maltose+builds acid from
44054506227N-acetylglucosamine+builds acid from
4405432528turanose+builds acid from
4405429016arginine+hydrolysis
44054581435-dehydro-D-gluconate+/-builds acid from
4405418305arbutin+/-builds acid from
4405418333D-arabitol+/-builds acid from
4405416988D-ribose+/-builds acid from
4405417814salicin+/-builds acid from
440544853esculin+/-hydrolysis
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose+builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837117992sucrose-builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117306maltose+builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
683714853esculin-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine+builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose+builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837112936D-galactose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose+builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from

metabolite production

@refChebi-IDmetaboliteproduction
4405416004(R)-lactateyes
4405416651(S)-lactateyes

enzymes

  • @ref: 44054
  • value: catalase
  • activity: -
  • ec: 1.11.1.6

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
24151-----+-----+++--------+-----+-----------+---------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentenrichment cultureenrichment culture temperature
24151fermented raw meat (industrial-type pure pork salami)GermanyDEUEurope
44054industrial pure pork salamiRogosa and MRS agar30

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production#Food
#Engineered#Food production#Meat

taxonmaps

  • @ref: 69479
  • File name: preview.99_134484.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_45;96_1756;97_2100;98_89770;99_134484&stattab=map
  • Last taxonomy: Companilactobacillus insicii subclade
  • 16S sequence: KP677494
  • Sequence Identity:
  • Total samples: 295
  • soil counts: 73
  • aquatic counts: 21
  • animal counts: 188
  • plant counts: 13

Safety information

risk assessment

  • @ref: 24151
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 24151
  • description: Lactobacillus insicii 16S ribosomal RNA gene, partial sequence
  • accession: KP677494
  • length: 1565
  • database: ena
  • NCBI tax ID: 1732567

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Companilactobacillus insicii DSM 29801GCA_003946015contigncbi1732567
66792Lactobacillus insicii strain DSM 298011732567.3wgspatric1732567
66792Companilactobacillus insicii DSM 298012881708693draftimg1732567

GC content

  • @ref: 24151
  • GC-content: 36.3
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno94.673yes
gram-positiveyes92.806yes
anaerobicno91.384yes
aerobicno97.027yes
halophileyes89.82no
spore-formingno95.687no
glucose-utilyes89.615no
flagellatedno98.206no
thermophileno98.759no
glucose-fermentyes89.582no

External links

@ref: 24151

culture collection no.: DSM 29801, CECT 8802

straininfo link

  • @ref: 90708
  • straininfo: 402533

literature

  • topic: Phylogeny
  • Pubmed-ID: 26486967
  • title: Lactobacillus insicii sp. nov., isolated from fermented raw meat.
  • authors: Ehrmann MA, Krockel L, Lick S, Radmann P, Bantleon A, Vogel RF
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.000705
  • year: 2015
  • mesh: Animals, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Dipeptides/chemistry, Fatty Acids/chemistry, Fermentation, *Food Microbiology, Lactobacillus/*cytology/genetics/isolation & purification, Meat Products/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Swine
  • topic2: Biotechnology

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
24151Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-29801Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 29801)
44054Matthias A. Ehrmann, Lothar Kröckel, Sonja Lick, Pia Radmann, Annegret Bantleon and Rudi. F. VogelLactobacillus insicii sp. nov., isolated from fermented raw meat10.1099/ijsem.0.000705IJSEM 66: 236-242 201626486967
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68371Automatically annotated from API 50CH acid
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
90708Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID402533.1