Strain identifier

BacDive ID: 132361

Type strain: Yes

Species: Comamonas phosphati

Strain Designation: WYH22-41

Strain history: <- F. Xie, Henan Key Lab. Microbial Engineering, Zhengzhou, China; WYH22-41

NCBI tax ID(s): 1211803 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 24150

BacDive-ID: 132361

DSM-Number: 26017

keywords: chemoorganotroph, facultative anaerobe, Bacteria, 16S sequence, genome sequence, mesophilic, Gram-negative, motile, rod-shaped, colony-forming

description: Comamonas phosphati WYH22-41 is a facultative anaerobe, chemoorganotroph, mesophilic bacterium that forms circular colonies and was isolated from phosphate rock powder at a phosphate-mining field.

NCBI tax id

  • NCBI tax id: 1211803
  • Matching level: species

strain history: <- F. Xie, Henan Key Lab. Microbial Engineering, Zhengzhou, China; WYH22-41

doi: 10.13145/bacdive132361.20220920.7

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Burkholderiales
  • family: Comamonadaceae
  • genus: Comamonas
  • species: Comamonas phosphati
  • full scientific name: Comamonas phosphati Xie et al. 2016

@ref: 24150

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Burkholderiales

family: Comamonadaceae

genus: Comamonas

species: Comamonas phosphati

full scientific name: Comamonas phosphati Xie et al. 2016

strain designation: WYH22-41

type strain: yes

Morphology

cell morphology

  • @ref: 44040
  • gram stain: negative
  • cell length: 0.9-1.5 µm
  • cell width: 0.4-0.7 µm
  • cell shape: rod-shaped
  • motility: yes
  • flagellum arrangement: polar

colony morphology

  • @ref: 44040
  • colony shape: circular
  • incubation period: 2 days
  • medium used: Luria Bertani agar

multimedia

  • @ref: 24150
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_26017.jpg
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
44040LB (Luria-Bertani) MEDIUMyes
44040Trypticase Soy Agar (TSA)yes
24150NUTRIENT AGAR (DSMZ Medium 1)yeshttps://bacmedia.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
44040positiveoptimum30mesophilic
44040positivegrowth4-35
24150positivegrowth28mesophilic

culture pH

@refabilitytypepH
44040positiveoptimum7
44040positivegrowth5.0-9.0

Physiology and metabolism

oxygen tolerance

  • @ref: 44040
  • oxygen tolerance: facultative anaerobe

nutrition type

  • @ref: 44040
  • type: chemoorganotroph

spore formation

  • @ref: 44040
  • type of spore: spore
  • spore formation: no

halophily

  • @ref: 44040
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-3 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4404027689decanoate-assimilation
4404016947citrate-assimilation
4404015824D-fructose-assimilation
4404016899D-mannitol-assimilation
4404016236ethanol-assimilation
4404030849L-arabinose-assimilation
4404016467L-arginine-assimilation
4404029991L-aspartate-assimilation
4404016643L-methionine-assimilation
4404017203L-proline-assimilation
4404062345L-rhamnose-assimilation
4404017115L-serine-assimilation
4404017266L-sorbose-assimilation
4404016857L-threonine-assimilation
4404017306maltose-assimilation
4404017790methanol-assimilation
44040506227N-acetylglucosamine-assimilation
4404017992sucrose-assimilation
4404027082trehalose-assimilation
4404018222xylose-assimilation
4404027613amygdalin-builds acid from
4404016899D-mannitol-builds acid from
4404017924D-sorbitol-builds acid from
4404017268myo-inositol-builds acid from
4404017992sucrose-builds acid from
4404016301nitrite-reduction
4404017128adipate+assimilation
4404017634D-glucose+assimilation
4404016024D-mannose+assimilation
4404024265gluconate+assimilation
4404017754glycerol+assimilation
4404016977L-alanine+assimilation
4404017196L-asparagine+assimilation
4404015971L-histidine+assimilation
4404015603L-leucine+assimilation
4404017295L-phenylalanine+assimilation
4404025115malate+assimilation
4404016634raffinose+assimilation
4404017108D-arabinose+builds acid from
4404017634D-glucose+builds acid from
4404062345L-rhamnose+builds acid from
4404028053melibiose+builds acid from
4404017632nitrate+reduction
440403557154-nitrophenyl beta-D-galactopyranoside+/-hydrolysis
440404853esculin+/-hydrolysis

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitiveis resistant
4404017698chloramphenicolyesyes
4404048923erythromycinyesyes
440406104kanamycinyesyes
440407507neomycinyesyes
44040100246norfloxacinyesyes
44040100241ciprofloxacinyesyes
4404018208penicillin gyesyes
4404028368novobiocinyesyes
4404028001vancomycinyesyes
4404028077rifampicinyesyes
4404028669bacitracinyesyes

metabolite production

@refChebi-IDmetaboliteproduction
4404016136hydrogen sulfideno
4404035581indoleno
4404015688acetoinyes

metabolite tests

  • @ref: 44040
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: +

enzymes

@refvalueactivityec
44040alkaline phosphatase+3.1.3.1
44040esterase (C 4)+
44040esterase Lipase (C 8)+
44040leucine arylamidase+3.4.11.1
44040acid phosphatase+3.1.3.2
44040naphthol-AS-BI-phosphohydrolase+
44040beta-galactosidase+/-3.2.1.23
44040beta-glucosidase+/-3.2.1.21
44040lipase (C 14)-
44040valine arylamidase-
44040cystine arylamidase-3.4.11.3
44040trypsin-3.4.21.4
44040alpha-chymotrypsin-3.4.21.1
44040alpha-galactosidase-3.2.1.22
44040beta-glucuronidase-3.2.1.31
44040alpha-glucosidase-3.2.1.20
44040N-acetyl-beta-glucosaminidase-3.2.1.52
44040alpha-mannosidase-3.2.1.24
44040alpha-fucosidase-3.2.1.51
44040cytochrome oxidase+1.9.3.1
44040catalase+1.11.1.6
44040tryptophan deaminase+4.1.99.1
44040arginine dihydrolase+3.5.3.6
44040urease+3.5.1.5
44040lysine decarboxylase-4.1.1.18
44040ornithine decarboxylase-4.1.1.17
44040gelatinase-
44040amylase-

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    44040C10:00.1
    44040C10:0 3OH3.4
    44040C12:02.9
    44040C14:00.4
    44040C14:0 2OH0.7
    44040C15:0 2OH0.1
    44040C16:038.1
    44040C16:0-2-OH3.7
    44040C16:1 2OH2.1
    44040C16:1ω6c / C16:1ω7c11.2
    44040C17:00.3
    44040C17:0-cyclo24.8
    44040C18:00.3
    44040C18:1ω6c / C18:1ω7c10
    44040C19:0-cycloω8c1.6
    44040C19:0 iso0.4
  • type of FA analysis: whole cell analysis
  • incubation medium: TSA
  • incubation temperature: 30
  • incubation time: 2
  • library/peak naming table: TSBA 6.0
  • system: MIS MIDI

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentenrichment cultureenrichment culture compositionenrichment culture durationenrichment culture temperature
24150phosphate rock powder at a phosphate-mining fieldYunnan Province, suburb of KunmingChinaCHNAsia
44040phosphate rock powder samples from a phosphate-mining fieldKumming, Yunnan ProvinceChinaCHNAsiaLBpH 7.2; 1 % tryptone, 1 % NaCl and 0.5 % yeast extract, all w/v4-5 days30

isolation source categories

Cat1Cat2Cat3
#Engineered#Other#Mine
#Environmental#Terrestrial#Geologic

Safety information

risk assessment

  • @ref: 24150
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 24150
  • description: Comamonas phosphati 16S ribosomal RNA gene, partial sequence
  • accession: JQ246447
  • length: 1490
  • database: ena
  • NCBI tax ID: 1211803

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Comamonas phosphati CGMCC 1.12294GCA_014637085scaffoldncbi1211803
66792Comamonas phosphati strain CGMCC 1.122941211803.3wgspatric1211803

GC content

  • @ref: 24150
  • GC-content: 62.4
  • method: thermal denaturation, midpoint method (Tm)

External links

@ref: 24150

culture collection no.: DSM 26017, CGMCC 1.12294

literature

  • topic: Phylogeny
  • Pubmed-ID: 26541594
  • title: Comamonas phosphati sp. nov., isolated from a phosphate mine.
  • authors: Xie F, Ma H, Quan S, Liu D, Chen G
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.000742
  • year: 2015
  • mesh: Bacterial Typing Techniques, Base Composition, China, Comamonas/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, *Mining, Molecular Sequence Data, Nucleic Acid Hybridization, *Phosphates, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Ubiquinone/chemistry
  • topic2: Genetics

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
24150Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-26017Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 26017)
44040Fuhong Xie, Huan Ma, Shujing Quan, Dehai Liu and Guocan ChenComamonas phosphati sp. nov., isolated from a phosphate mine10.1099/ijsem.0.000742IJSEM 66: 456-461 201626541594
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes