Strain identifier
BacDive ID: 132360
Type strain:
Species: Vibrio galatheae
Strain Designation: S2757
Strain history: <- S. Giubergia, Technical University of Denmark, Kgs. Lyngby; S2757 <- L. Gram, Technical University of Denmark
NCBI tax ID(s): 579748 (species)
General
@ref: 24149
BacDive-ID: 132360
DSM-Number: 100497
keywords: genome sequence, Bacteria, facultative anaerobe, Gram-negative, motile, rod-shaped, colony-forming
description: Vibrio galatheae S2757 is a facultative anaerobe, Gram-negative, motile bacterium that forms circular colonies and was isolated from mussel collected in the sea.
NCBI tax id
- NCBI tax id: 579748
- Matching level: species
strain history
- @ref: 24149
- history: <- S. Giubergia, Technical University of Denmark, Kgs. Lyngby; S2757 <- L. Gram, Technical University of Denmark
doi: 10.13145/bacdive132360.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Vibrionales
- family: Vibrionaceae
- genus: Vibrio
- species: Vibrio galatheae
- full scientific name: Vibrio galatheae Giubergia et al. 2016
@ref: 24149
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Vibrionales, not validated
family: Vibrionaceae
genus: Vibrio
species: Vibrio galatheae
full scientific name: Vibrio galatheae Giubergia et al. 2016
strain designation: S2757
type strain: yes
Morphology
cell morphology
- @ref: 44003
- gram stain: negative
- cell shape: rod-shaped
- motility: yes
- flagellum arrangement: polar
colony morphology
@ref | colony size | colony color | colony shape | incubation period | medium used |
---|---|---|---|---|---|
44003 | 2-4 mm | beige | circular | 2 days | marine agar |
44003 | 2-4 mm | green | 2 days | thiosulfate-citrate-bile-sucrose |
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
44003 | Marine agar (MA) | yes | ||
44003 | thiosulfate-citrate-bile-sucrose | yes | ||
24149 | BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) | yes | https://mediadive.dsmz.de/medium/514 | Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
44003 | positive | growth | 15-40 | |
44003 | positive | optimum | 30 | mesophilic |
24149 | positive | growth | 25 | mesophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 44003
- oxygen tolerance: facultative anaerobe
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
44003 | NaCl | positive | growth | 0.5-8 % |
44003 | NaCl | positive | optimum | 2-5 % |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
44003 | 86537 | 2,2-dimethylsuccinic acid | - | assimilation |
44003 | 17754 | glycerol | - | assimilation |
44003 | 73804 | glycyl L-aspartic acid | - | assimilation |
44003 | 17203 | L-proline | - | assimilation |
44003 | 16857 | L-threonine | - | assimilation |
44003 | 28037 | N-acetylgalactosamine | - | assimilation |
44003 | 17992 | sucrose | - | assimilation |
44003 | 16947 | citrate | + | builds acid from |
44003 | 25115 | malate | + | builds acid from |
44003 | 17925 | alpha-D-glucose | + | carbon source |
44003 | 17665 | alpha-D-glucose 6-phosphate | + | carbon source |
44003 | 17057 | cellobiose | + | carbon source |
44003 | 15570 | D-alanine | + | carbon source |
44003 | 15824 | D-fructose | + | carbon source |
44003 | 8391 | D-gluconate | + | carbon source |
44003 | 14314 | D-glucose 6-phosphate | + | carbon source |
44003 | 16899 | D-mannitol | + | carbon source |
44003 | 16024 | D-mannose | + | carbon source |
44003 | 17596 | inosine | + | carbon source |
44003 | L-alanine 4-nitroanilide | + | carbon source | |
44003 | 73786 | L-alanylglycine | + | carbon source |
44003 | 17196 | L-asparagine | + | carbon source |
44003 | 29991 | L-aspartate | + | carbon source |
44003 | 24996 | lactate | + | carbon source |
44003 | 17306 | maltose | + | carbon source |
44003 | 506227 | N-acetylglucosamine | + | carbon source |
44003 | 73784 | glycyl-l-glutamate | + | carbon source |
44003 | 17748 | thymidine | + | carbon source |
44003 | 27082 | trehalose | + | carbon source |
44003 | 4853 | esculin | + | hydrolysis |
44003 | 17632 | nitrate | + | reduction |
68369 | 25115 | malate | + | assimilation |
68369 | 17128 | adipate | - | assimilation |
68369 | 27689 | decanoate | - | assimilation |
68369 | 24265 | gluconate | + | assimilation |
68369 | 17306 | maltose | + | assimilation |
68369 | 59640 | N-acetylglucosamine | + | assimilation |
68369 | 16899 | D-mannitol | + | assimilation |
68369 | 16024 | D-mannose | + | assimilation |
68369 | 30849 | L-arabinose | - | assimilation |
68369 | 17634 | D-glucose | + | assimilation |
68369 | 4853 | esculin | + | hydrolysis |
68369 | 16199 | urea | - | hydrolysis |
68369 | 17634 | D-glucose | + | fermentation |
68369 | 27897 | tryptophan | + | energy source |
68369 | 17632 | nitrate | + | reduction |
antibiotic resistance
- @ref: 44003
- ChEBI: 73908
- metabolite: vibriostat
- is antibiotic: yes
- is sensitive: yes
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
44003 | 35581 | indole | yes |
68369 | 35581 | indole | yes |
metabolite tests
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- indole test: +
enzymes
@ref | value | activity | ec |
---|---|---|---|
44003 | catalase | + | 1.11.1.6 |
44003 | cytochrome oxidase | + | 1.9.3.1 |
44003 | alkaline phosphatase | + | 3.1.3.1 |
44003 | esterase Lipase (C 8) | + | |
44003 | leucine arylamidase | + | 3.4.11.1 |
44003 | valine arylamidase | + | |
44003 | cystine arylamidase | + | 3.4.11.3 |
44003 | lipase | - | |
44003 | acid phosphatase | - | 3.1.3.2 |
68369 | cytochrome oxidase | + | 1.9.3.1 |
68369 | beta-glucosidase | + | 3.2.1.21 |
68369 | urease | - | 3.5.1.5 |
fatty acid profile
fatty acids
@ref fatty acid percentage 44003 C16:0 19.5 44003 C18:1ω7c and/or C18:1ω6c 15.8 44003 C15:0 iso 2.1 44003 C16:0 iso 3.7 44003 C17:0 iso 4 44003 one or more of C16:1ω7c, C16:1ω6c and/ 34.7 - type of FA analysis: whole cell analysis
- incubation medium: marine agar
- agar/liquid: agar
- incubation temperature: 25
- incubation time: 1
- system: MIS MIDI
API 20NE
@ref | NO3 | TRP | GLU_ Ferm | ADH Arg | URE | ESC | GEL | PNPG | GLU_ Assim | ARA | MNE | MAN | NAG | MAL | GNT | CAP | ADI | MLT | CIT | PAC | OX |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
24149 | + | + | + | + | - | + | + | + | + | - | + | + | + | + | + | - | - | + | - | + | + |
24149 | + | + | + | - | - | + | - | + | + | - | + | + | + | + | + | - | - | + | - | + | + |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent |
---|---|---|---|---|---|
24149 | mussel collected in the sea | Solomon Sea | Solomon Islands | SLB | Australia and Oceania |
44003 | a mussel | Solomon Sea | Solomon Islands | SLB | Australia and Oceania |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Aquatic | #Marine |
#Host | #Invertebrates (Other) | #Mollusca |
Safety information
risk assessment
- @ref: 24149
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Vibrio galatheae S2757 | GCA_000967545 | contig | ncbi | 579748 |
66792 | Vibrio galatheae S2757 | 2791355472 | draft | img | 579748 |
GC content
@ref | GC-content | method |
---|---|---|
44003 | 45.3 | genome sequence analysis |
24149 | 45.3 | sequence analysis |
External links
@ref: 24149
culture collection no.: DSM 100497, LMG 28895
straininfo link
- @ref: 90706
- straininfo: 397178
literature
- topic: Phylogeny
- Pubmed-ID: 26514698
- title: Vibrio galatheae sp. nov., a member of the family Vibrionaceae isolated from a mussel.
- authors: Giubergia S, Machado H, Valentina Mateiu R, Gram L
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijsem.0.000723
- year: 2015
- mesh: Animals, Bacterial Typing Techniques, Base Composition, Bivalvia/*microbiology, DNA, Bacterial/genetics, Fatty Acids/chemistry, Genes, Bacterial, Melanesia, Molecular Sequence Data, Multilocus Sequence Typing, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vibrio/*classification/genetics/isolation & purification
- topic2: Genetics
Reference
@id | authors | title | doi/url | catalogue | journal | pubmed |
---|---|---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |||
24149 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-100497 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 100497) | |||
44003 | Sonia Giubergia, Henrique Machado, Ramona Valentina Mateiu, Lone Gram | Vibrio galatheae sp. nov., a member of the family Vibrionaceae isolated from a mussel | 10.1099/ijsem.0.000723 | IJSEM 66: 347-352 2016 | 26514698 | |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |||
68369 | Automatically annotated from API 20NE | |||||
90706 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID397178.1 |