Strain identifier

BacDive ID: 132359

Type strain: Yes

Species: Thermanaeromonas burensis

Strain Designation: IA 106, IA106

Strain history: Z. Ben Ali Gam; IRD, France; IA106.

NCBI tax ID(s): 1226730 (species)

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General

@ref: 24148

BacDive-ID: 132359

DSM-Number: 26576

keywords: 16S sequence, Bacteria, anaerobe, halotolerant, spore-forming, thermophilic, rod-shaped

description: Thermanaeromonas burensis IA 106 is an anaerobe, halotolerant, spore-forming bacterium that was isolated from seepage water from a subterranean Callovo-Oxfordian clay formation at a depth of 490 m.

NCBI tax id

  • NCBI tax id: 1226730
  • Matching level: species

strain history

@refhistory
24148<- Z. Ben Ali Gam, Lab. Microbiol. de IRD, Marseille, France; IA106 <- Z. Ben Ali Gam {2011}
67770Z. Ben Ali Gam; IRD, France; IA106.

doi: 10.13145/bacdive132359.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Thermoanaerobacterales
  • family: Thermoanaerobacteraceae
  • genus: Thermanaeromonas
  • species: Thermanaeromonas burensis
  • full scientific name: Thermanaeromonas burensis Gam et al. 2016

@ref: 24148

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Thermoanaerobacterales

family: Thermoanaerobacteraceae

genus: Thermanaeromonas

species: Thermanaeromonas burensis

full scientific name: Thermanaeromonas burensis Gam et al. 2016

strain designation: IA 106, IA106

type strain: yes

Morphology

cell morphology

@refcell lengthcell widthcell shapemotilitygram stain
440012-6 µm0.5 µmrod-shaped
44001no
44001positive

colony morphology

  • @ref: 44001
  • medium used: Basal medium

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
44001Basal mediumyesyeast extract (1 g/l), glucose (20 mM) with thiosulfate as electron acceptor
24148THERMOANAEROMONAS MEDIUM (DSMZ Medium 963)yesName: THERMOANAEROMONAS MEDIUM (DSMZ Medium 963) Composition: NaHCO3 4.9456 g/l Na2S2O3 x 5 H2O 1.22651 g/l K2HPO4 0.771513 g/l KH2PO4 0.74184 g/l Yeast extract 0.49456 g/l D-Glucose 0.49456 g/l NH4Cl 0.49456 g/l L-Cysteine HCl x H2O 0.24728 g/l MgSO4 x 7 H2O 0.24728 g/l Nitrilotriacetic acid 0.126607 g/l Na3-EDTA 0.0395648 g/l NaCl 0.0098912 g/l FeSO4 x 7 H2O 0.0098912 g/l FeCl2 x 4 H2O 0.0098912 g/l CaCl2 x 2 H2O 0.00098912 g/l MnCl2 x 4 H2O 0.00098912 g/l ZnCl2 0.00098912 g/l NiCl2 x 6 H2O 0.00098912 g/l Sodium resazurin 0.00049456 g/l Na2WO4 x 2 H2O 0.000395648 g/l Na2SeO3 x 5 H2O 0.000296736 g/l CoCl2 x 6 H2O 0.000296736 g/l Na2MoO4 x 2 H2O 0.000296736 g/l CuCl2 0.000197824 g/l H3BO3 9.8912e-05 g/l Pyridoxine hydrochloride 9.8912e-05 g/l Riboflavin 4.9456e-05 g/l Thiamine HCl 4.9456e-05 g/l Calcium D-(+)-pantothenate 4.9456e-05 g/l p-Aminobenzoic acid 4.9456e-05 g/l (DL)-alpha-Lipoic acid 4.9456e-05 g/l Nicotinic acid 4.9456e-05 g/l Folic acid 1.97824e-05 g/l Biotin 1.97824e-05 g/l Vitamin B12 9.8912e-07 g/l Distilled waterhttps://mediadive.dsmz.de/medium/963

culture temp

@refgrowthtypetemperaturerange
44001positivegrowth55-70thermophilic
44001positiveoptimum65thermophilic
24148positivegrowth65thermophilic
67770positivegrowth65thermophilic

culture pH

@refabilitytypepHPH range
44001positivegrowth5.5-9.0alkaliphile
44001positiveoptimum7.5

Physiology and metabolism

oxygen tolerance

  • @ref: 44001
  • oxygen tolerance: anaerobe

spore formation

  • @ref: 44001
  • spore description: terminal endospores
  • type of spore: endospore
  • spore formation: yes

halophily

@refhalophily levelsaltgrowthtested relationconcentration
44001halotolerantNaClpositivegrowth0-20 g/L
44001NaClpositiveoptimum1 g/L

observation

  • @ref: 44001
  • observation: occurred singly or in pairs, cell wall composed of three dense layers (two thick and a thinner middle layer) separated by two light spaces

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4400133403elemental sulfur-electron acceptor
4400129806fumarate-electron acceptor
4400117632nitrate-electron acceptor
4400116301nitrite-electron acceptor
4400116189sulfate-electron acceptor
4400117359sulfite-electron acceptor
4400116094thiosulfate+electron acceptor

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    44001C15:0 anteiso9.3
    44001C16:015.9
    44001C18:015.4
    44001C15:0 iso13
    44001C16:0 iso8.7
    44001C17:0 iso14.7
    44001iso-C17:1 I and/or anteiso-C 17:1 B14.8
  • type of FA analysis: whole cell analysis
  • system: MIS MIDI
  • method/protocol: Sasser, 1990
  • cutoff value:

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentenrichment culture compositionenrichment culture durationenrichment culture temperatureisolation procedure
24148seepage water from a subterranean Callovo-Oxfordian clay formation at a depth of 490 mnorthern partFranceFRAEurope
44001seepage water collected in a metal biocorrosion test at a depth of 490 msubterranean Callovo-Oxfordian clay formation in northern FranceFranceFRAEuropethiosulfate-reducing bacteria medium2 weeks60in Hungate tubes containing 5 ml medium
67770Seepage water collected in a metal biocorrosion test at a depth of 490 m, in a subterranean Callovo-Oxfordian clay formation in northern FranceFranceFRAEurope

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic
#Environmental#Terrestrial
#Engineered#Industrial
#Engineered#Built environment#Water reservoir (Aquarium/pool)

taxonmaps

  • @ref: 69479
  • File name: preview.99_57994.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_170;96_23056;97_32222;98_41306;99_57994&stattab=map
  • Last taxonomy: Thermanaeromonas burensis subclade
  • 16S sequence: JQ446369
  • Sequence Identity:
  • Total samples: 155
  • soil counts: 52
  • aquatic counts: 73
  • animal counts: 20
  • plant counts: 10

Safety information

risk assessment

  • @ref: 24148
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 24148
  • description: Thermanaeromonas burensis 16S ribosomal RNA gene, partial sequence
  • accession: JQ446369
  • length: 1537
  • database: ena
  • NCBI tax ID: 1226730

GC content

  • @ref: 24148
  • GC-content: 60.2
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 24148

culture collection no.: DSM 26576, JCM 18718

straininfo link

  • @ref: 90705
  • straininfo: 397134

literature

  • topic: Phylogeny
  • Pubmed-ID: 26541283
  • title: Thermanaeromonas burensis sp. nov., a thermophilic anaerobe isolated from a subterranean clay environment.
  • authors: Gam ZBA, Daumas S, Casalot L, Bartoli-Joseph M, Necib S, Linard Y, Labat M
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.000739
  • year: 2015
  • mesh: Aluminum Silicates, Bacteria, Anaerobic/classification/genetics/isolation & purification, Base Composition, Clay, DNA, Bacterial/genetics, Fatty Acids/chemistry, Firmicutes/*classification/genetics/isolation & purification, France, Fresh Water/*microbiology, Molecular Sequence Data, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
  • topic2: Enzymology

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
24148Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-26576Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 26576)
44001Zouhaier Ben Ali Gam, Sylvie Daumas, Laurence Casalot, Manon Bartoli-Joseph, Sophia Necib, Yannick Linard, Marc LabatThermanaeromonas burensis sp. nov., a thermophilic anaerobe isolated from a subterranean clay environment10.1099/ijsem.0.000739IJSEM 66: 445-449 201626541283
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
90705Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID397134.1