Strain identifier
BacDive ID: 1323
Type strain:
Species: Bacillus cytotoxicus
Strain Designation: 391-98
Strain history: CIP <- 2009, D. Lereclus, INRA, Jouy en Josas, France <- M.L. de Buyser, AFSSA LERQAP, Maisons-Alfort, France: strain NVH 391-98
NCBI tax ID(s): 315749 (strain), 580165 (species)
General
@ref: 16614
BacDive-ID: 1323
DSM-Number: 22905
keywords: 16S sequence, Bacteria, facultative anaerobe, spore-forming, mesophilic, Gram-positive, motile, rod-shaped, colony-forming, human pathogen
description: Bacillus cytotoxicus 391-98 is a facultative anaerobe, spore-forming, mesophilic human pathogen that forms circular colonies and was isolated from vegetable puree, related to food poisoning.
NCBI tax id
NCBI tax id | Matching level |
---|---|
315749 | strain |
580165 | species |
strain history
@ref | history |
---|---|
16614 | <- M. Contzen, Chemisches und Veterinäruntersuchungsamt (CVUA), Stuttgart, Fellbach, Germany; NVH391-98 <- A. Sorokin, INRA, France <- M.-L. De Buyser, Afssa, France |
118837 | CIP <- 2009, D. Lereclus, INRA, Jouy en Josas, France <- M.L. de Buyser, AFSSA LERQAP, Maisons-Alfort, France: strain NVH 391-98 |
doi: 10.13145/bacdive1323.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Caryophanales
- family: Bacillaceae
- genus: Bacillus
- species: Bacillus cytotoxicus
- full scientific name: Bacillus cytotoxicus Guinebretière et al. 2013
@ref: 16614
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Bacillales
family: Bacillaceae
genus: Bacillus
species: Bacillus cytotoxicus
full scientific name: Bacillus cytotoxicus Guinebretière et al. 2013
strain designation: 391-98
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell length | cell shape | motility |
---|---|---|---|---|
23142 | positive | >1.0 µm | rod-shaped | yes |
118837 | positive | rod-shaped | no |
colony morphology
- @ref: 23142
- colony size: 1.0 mm
- colony color: cream
- colony shape: circular
- incubation period: 1 day
- medium used: TSA
Culture and growth conditions
culture medium
@ref | name | growth | composition | link |
---|---|---|---|---|
23142 | Trypticase Soy Agar (TSA) | yes | ||
33672 | MEDIUM 72- for trypto casein soja agar | yes | Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g) | |
16614 | NUTRIENT AGAR (DSMZ Medium 1) | yes | Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water | https://mediadive.dsmz.de/medium/1 |
16614 | CASO AGAR (MERCK 105458) (DSMZ Medium 220) | yes | Name: CASO AGAR (Merck 105458) (DSMZ Medium 220; with strain-specific modifications) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l MnSO4 0.01 g/l Distilled water | https://mediadive.dsmz.de/medium/220 |
118837 | CIP Medium 72 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
16614 | positive | growth | 37 | mesophilic |
23142 | positive | maximum | 50 | thermophilic |
23142 | positive | minimum | 20 | psychrophilic |
23142 | positive | optimum | 30-37 | mesophilic |
33672 | positive | growth | 30 | mesophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 118837
- oxygen tolerance: facultative anaerobe
spore formation
- @ref: 118837
- spore formation: yes
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
23142 | 28087 | glycogen | - | assimilation |
23142 | 17992 | sucrose | - | assimilation |
23142 | 27082 | trehalose | - | assimilation |
23142 | 28260 | galactose | - | carbon source |
23142 | 28066 | gentiobiose | - | carbon source |
23142 | 24265 | gluconate | - | carbon source |
23142 | 17754 | glycerol | - | carbon source |
23142 | 28087 | glycogen | - | carbon source |
23142 | 37657 | methyl D-glucoside | - | carbon source |
23142 | 28017 | starch | - | carbon source |
23142 | 17992 | sucrose | - | carbon source |
23142 | 27082 | trehalose | - | carbon source |
23142 | 27897 | tryptophan | - | carbon source |
23142 | 32528 | turanose | - | carbon source |
23142 | 27897 | tryptophan | - | energy source |
23142 | 28017 | starch | - | hydrolysis |
23142 | 16136 | hydrogen sulfide | - | other |
23142 | 16024 | D-mannose | + | assimilation |
23142 | 18305 | arbutin | + | carbon source |
23142 | 17057 | cellobiose | + | carbon source |
23142 | 16024 | D-mannose | + | carbon source |
23142 | 506227 | N-acetylglucosamine | + | carbon source |
23142 | 33942 | ribose | + | carbon source |
23142 | 17814 | salicin | + | carbon source |
23142 | 17632 | nitrate | + | reduction |
23142 | 5291 | gelatin | + | |
23142 | 27613 | amygdalin | +/- | carbon source |
118837 | 17632 | nitrate | + | reduction |
118837 | 16301 | nitrite | + | reduction |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
23142 | 35581 | indole | no |
23142 | 15688 | acetoin | yes |
118837 | 35581 | indole | no |
metabolite tests
@ref | Chebi-ID | metabolite | voges-proskauer-test | indole test |
---|---|---|---|---|
23142 | 15688 | acetoin | + | |
23142 | 35581 | indole | - |
enzymes
@ref | value | activity | ec |
---|---|---|---|
23142 | lysine decarboxylase | - | 4.1.1.18 |
23142 | ornithine decarboxylase | - | 4.1.1.17 |
23142 | tryptophan deaminase | - | 4.1.99.1 |
23142 | urease | - | 3.5.1.5 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | beta-glucosidase | + | 3.2.1.21 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | alpha-chymotrypsin | + | 3.4.21.1 |
68382 | trypsin | - | 3.4.21.4 |
68382 | cystine arylamidase | + | 3.4.11.3 |
68382 | valine arylamidase | + | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | lipase (C 14) | - | |
68382 | esterase lipase (C 8) | - | |
68382 | esterase (C 4) | + | |
68382 | alkaline phosphatase | - | 3.1.3.1 |
118837 | oxidase | + | |
118837 | catalase | + | 1.11.1.6 |
118837 | urease | - | 3.5.1.5 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
118837 | - | - | + | - | - | + | + | + | - | + | + | + | - | - | - | - | + | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | country | origin.country | continent | isolation date |
---|---|---|---|---|---|
16614 | vegetable puree, related to food poisoning | France | FRA | Europe | |
118837 | Food, Vegetable | France | FRA | Europe | 1998 |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Engineered | #Food production | #Food |
#Host | #Plants | |
#Host Body-Site | #Plant | #Fruit (Seed) |
Safety information
risk assessment
@ref | pathogenicity human | biosafety level | biosafety level comment |
---|---|---|---|
16614 | yes, in single cases | 1 | Risk group (German classification) |
118837 | 1 | Risk group (French classification) |
Sequence information
16S sequences
- @ref: 16614
- description: Bacillus cereus partial 16S rRNA gene and ITS1, strain NVH 391-98
- accession: AM747234
- length: 1765
- database: ena
- NCBI tax ID: 1396
GC content
- @ref: 16614
- GC-content: 35.8
- method: high performance liquid chromatography (HPLC)
External links
@ref: 16614
culture collection no.: DSM 22905, CIP 110041, NVH 391-98, WDCM 00220
straininfo link
- @ref: 70973
- straininfo: 378380
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Genetics | 17434157 | Extending the Bacillus cereus group genomics to putative food-borne pathogens of different toxicity. | Lapidus A, Goltsman E, Auger S, Galleron N, Segurens B, Dossat C, Land ML, Broussolle V, Brillard J, Guinebretiere MH, Sanchis V, Nguen-The C, Lereclus D, Richardson P, Wincker P, Weissenbach J, Ehrlich SD, Sorokin A | Chem Biol Interact | 10.1016/j.cbi.2007.03.003 | 2007 | Bacillus cereus/*genetics, *Food Microbiology, Foodborne Diseases/*microbiology, *Genome, Bacterial, Humans | Biotechnology |
Phylogeny | 22328607 | Bacillus cytotoxicus sp. nov. is a novel thermotolerant species of the Bacillus cereus Group occasionally associated with food poisoning. | Guinebretiere MH, Auger S, Galleron N, Contzen M, De Sarrau B, De Buyser ML, Lamberet G, Fagerlund A, Granum PE, Lereclus D, De Vos P, Nguyen-The C, Sorokin A | Int J Syst Evol Microbiol | 10.1099/ijs.0.030627-0 | 2012 | Bacillus/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/analysis, *Foodborne Diseases, France, Multilocus Sequence Typing, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA | Transcriptome |
Metabolism | 26186121 | Is Cytotoxin K from Bacillus cereus a bona fide enterotoxin? | Castiaux V, Liu X, Delbrassinne L, Mahillon J | Int J Food Microbiol | 10.1016/j.ijfoodmicro.2015.06.020 | 2015 | Bacillus cereus/genetics/*metabolism, Cytotoxins/genetics/*metabolism, Diarrhea/microbiology, Enterotoxins/genetics/*metabolism, Foodborne Diseases, Humans, Multilocus Sequence Typing, Virulence Factors/genetics/*metabolism | Pathogenicity |
Genetics | 26294623 | Draft Genome Sequence of Bacillus cytotoxicus CVUAS 2833, a Very Close Relative to Type Strain NVH 391-98 Isolated from a Different Location. | Bohm ME, Huptas C, Krey VM, Scherer S | Genome Announc | 10.1128/genomeA.00901-15 | 2015 | Phylogeny | |
Genetics | 32855256 | Draft Genome Sequence of CH_213, a Highly Cytotoxic Bacillus cytotoxicus Strain Isolated from Mashed Potatoes. | Stevens MJA, Johler S | Microbiol Resour Announc | 10.1128/MRA.00836-20 | 2020 | Phylogeny | |
Genetics | 34992589 | Bacillus cytotoxicus Genomics: Chromosomal Diversity and Plasmidome Versatility. | Fayad N, Kone KM, Gillis A, Mahillon J | Front Microbiol | 10.3389/fmicb.2021.789929 | 2021 |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
16614 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 22905) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-22905 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
23142 | Marie-Hélène Guinebretière, Sandrine Auger, Nathalie Galleron, Matthias Contzen, Benoit De Sarrau, Marie-Laure De Buyser, Gilles Lamberet, Annette Fagerlund, Per Einar Granum, Didier Lereclus, Paul De Vos, Christophe Nguyen-The, Alexei Sorokin | 10.1099/ijs.0.030627-0 | Bacillus cytotoxicus sp. nov. is a novel thermotolerant species of the Bacillus cereus Group occasionally associated with food poisoning | IJSEM 63: 31-40 2013 | 22328607 | |
33672 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/7854 | ||||
68382 | Automatically annotated from API zym | |||||
70973 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID378380.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
118837 | Curators of the CIP | Collection of Institut Pasteur (CIP 110041) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20110041 |