Strain identifier

BacDive ID: 1323

Type strain: Yes

Species: Bacillus cytotoxicus

Strain Designation: 391-98

Strain history: CIP <- 2009, D. Lereclus, INRA, Jouy en Josas, France <- M.L. de Buyser, AFSSA LERQAP, Maisons-Alfort, France: strain NVH 391-98

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General

@ref: 16614

BacDive-ID: 1323

DSM-Number: 22905

keywords: 16S sequence, Bacteria, facultative anaerobe, spore-forming, mesophilic, Gram-positive, motile, rod-shaped, colony-forming, human pathogen

description: Bacillus cytotoxicus 391-98 is a facultative anaerobe, spore-forming, mesophilic human pathogen that forms circular colonies and was isolated from vegetable puree, related to food poisoning.

NCBI tax id

NCBI tax idMatching level
315749strain
580165species

strain history

@refhistory
16614<- M. Contzen, Chemisches und Veterinäruntersuchungsamt (CVUA), Stuttgart, Fellbach, Germany; NVH391-98 <- A. Sorokin, INRA, France <- M.-L. De Buyser, Afssa, France
118837CIP <- 2009, D. Lereclus, INRA, Jouy en Josas, France <- M.L. de Buyser, AFSSA LERQAP, Maisons-Alfort, France: strain NVH 391-98

doi: 10.13145/bacdive1323.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Bacillus
  • species: Bacillus cytotoxicus
  • full scientific name: Bacillus cytotoxicus Guinebretière et al. 2013

@ref: 16614

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Bacillus

species: Bacillus cytotoxicus

full scientific name: Bacillus cytotoxicus Guinebretière et al. 2013

strain designation: 391-98

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell shapemotility
23142positive>1.0 µmrod-shapedyes
118837positiverod-shapedno

colony morphology

  • @ref: 23142
  • colony size: 1.0 mm
  • colony color: cream
  • colony shape: circular
  • incubation period: 1 day
  • medium used: TSA

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
23142Trypticase Soy Agar (TSA)yes
33672MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
16614NUTRIENT AGAR (DSMZ Medium 1)yesName: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled waterhttps://mediadive.dsmz.de/medium/1
16614CASO AGAR (MERCK 105458) (DSMZ Medium 220)yesName: CASO AGAR (Merck 105458) (DSMZ Medium 220; with strain-specific modifications) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l MnSO4 0.01 g/l Distilled waterhttps://mediadive.dsmz.de/medium/220
118837CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
16614positivegrowth37mesophilic
23142positivemaximum50thermophilic
23142positiveminimum20psychrophilic
23142positiveoptimum30-37mesophilic
33672positivegrowth30mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 118837
  • oxygen tolerance: facultative anaerobe

spore formation

  • @ref: 118837
  • spore formation: yes

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2314228087glycogen-assimilation
2314217992sucrose-assimilation
2314227082trehalose-assimilation
2314228260galactose-carbon source
2314228066gentiobiose-carbon source
2314224265gluconate-carbon source
2314217754glycerol-carbon source
2314228087glycogen-carbon source
2314237657methyl D-glucoside-carbon source
2314228017starch-carbon source
2314217992sucrose-carbon source
2314227082trehalose-carbon source
2314227897tryptophan-carbon source
2314232528turanose-carbon source
2314227897tryptophan-energy source
2314228017starch-hydrolysis
2314216136hydrogen sulfide-other
2314216024D-mannose+assimilation
2314218305arbutin+carbon source
2314217057cellobiose+carbon source
2314216024D-mannose+carbon source
23142506227N-acetylglucosamine+carbon source
2314233942ribose+carbon source
2314217814salicin+carbon source
2314217632nitrate+reduction
231425291gelatin+
2314227613amygdalin+/-carbon source
11883717632nitrate+reduction
11883716301nitrite+reduction

metabolite production

@refChebi-IDmetaboliteproduction
2314235581indoleno
2314215688acetoinyes
11883735581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
2314215688acetoin+
2314235581indole-

enzymes

@refvalueactivityec
23142lysine decarboxylase-4.1.1.18
23142ornithine decarboxylase-4.1.1.17
23142tryptophan deaminase-4.1.99.1
23142urease-3.5.1.5
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin+3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase+3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
118837oxidase+
118837catalase+1.11.1.6
118837urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
118837--+--+++-+++----+---

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentisolation date
16614vegetable puree, related to food poisoningFranceFRAEurope
118837Food, VegetableFranceFRAEurope1998

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production#Food
#Host#Plants
#Host Body-Site#Plant#Fruit (Seed)

Safety information

risk assessment

@refpathogenicity humanbiosafety levelbiosafety level comment
16614yes, in single cases1Risk group (German classification)
1188371Risk group (French classification)

Sequence information

16S sequences

  • @ref: 16614
  • description: Bacillus cereus partial 16S rRNA gene and ITS1, strain NVH 391-98
  • accession: AM747234
  • length: 1765
  • database: ena
  • NCBI tax ID: 1396

GC content

  • @ref: 16614
  • GC-content: 35.8
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 16614

culture collection no.: DSM 22905, CIP 110041, NVH 391-98, WDCM 00220

straininfo link

  • @ref: 70973
  • straininfo: 378380

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Genetics17434157Extending the Bacillus cereus group genomics to putative food-borne pathogens of different toxicity.Lapidus A, Goltsman E, Auger S, Galleron N, Segurens B, Dossat C, Land ML, Broussolle V, Brillard J, Guinebretiere MH, Sanchis V, Nguen-The C, Lereclus D, Richardson P, Wincker P, Weissenbach J, Ehrlich SD, Sorokin AChem Biol Interact10.1016/j.cbi.2007.03.0032007Bacillus cereus/*genetics, *Food Microbiology, Foodborne Diseases/*microbiology, *Genome, Bacterial, HumansBiotechnology
Phylogeny22328607Bacillus cytotoxicus sp. nov. is a novel thermotolerant species of the Bacillus cereus Group occasionally associated with food poisoning.Guinebretiere MH, Auger S, Galleron N, Contzen M, De Sarrau B, De Buyser ML, Lamberet G, Fagerlund A, Granum PE, Lereclus D, De Vos P, Nguyen-The C, Sorokin AInt J Syst Evol Microbiol10.1099/ijs.0.030627-02012Bacillus/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/analysis, *Foodborne Diseases, France, Multilocus Sequence Typing, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNATranscriptome
Metabolism26186121Is Cytotoxin K from Bacillus cereus a bona fide enterotoxin?Castiaux V, Liu X, Delbrassinne L, Mahillon JInt J Food Microbiol10.1016/j.ijfoodmicro.2015.06.0202015Bacillus cereus/genetics/*metabolism, Cytotoxins/genetics/*metabolism, Diarrhea/microbiology, Enterotoxins/genetics/*metabolism, Foodborne Diseases, Humans, Multilocus Sequence Typing, Virulence Factors/genetics/*metabolismPathogenicity
Genetics26294623Draft Genome Sequence of Bacillus cytotoxicus CVUAS 2833, a Very Close Relative to Type Strain NVH 391-98 Isolated from a Different Location.Bohm ME, Huptas C, Krey VM, Scherer SGenome Announc10.1128/genomeA.00901-152015Phylogeny
Genetics32855256Draft Genome Sequence of CH_213, a Highly Cytotoxic Bacillus cytotoxicus Strain Isolated from Mashed Potatoes.Stevens MJA, Johler SMicrobiol Resour Announc10.1128/MRA.00836-202020Phylogeny
Genetics34992589Bacillus cytotoxicus Genomics: Chromosomal Diversity and Plasmidome Versatility.Fayad N, Kone KM, Gillis A, Mahillon JFront Microbiol10.3389/fmicb.2021.7899292021

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
16614Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 22905)https://www.dsmz.de/collection/catalogue/details/culture/DSM-22905
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23142Marie-Hélène Guinebretière, Sandrine Auger, Nathalie Galleron, Matthias Contzen, Benoit De Sarrau, Marie-Laure De Buyser, Gilles Lamberet, Annette Fagerlund, Per Einar Granum, Didier Lereclus, Paul De Vos, Christophe Nguyen-The, Alexei Sorokin10.1099/ijs.0.030627-0Bacillus cytotoxicus sp. nov. is a novel thermotolerant species of the Bacillus cereus Group occasionally associated with food poisoningIJSEM 63: 31-40 201322328607
33672Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/7854
68382Automatically annotated from API zym
70973Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID378380.1StrainInfo: A central database for resolving microbial strain identifiers
118837Curators of the CIPCollection of Institut Pasteur (CIP 110041)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20110041