Strain identifier

BacDive ID: 132199

Type strain: Yes

Species: Acinetobacter seifertii

Strain Designation: Gerner-Smidt 10090

Strain history: CIP <- 2012, A. Nemec, Inst. Pasteur, Paris, France: strain NIPH 973

NCBI tax ID(s): 1530123 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 23988

BacDive-ID: 132199

DSM-Number: 102854

keywords: genome sequence, 16S sequence, Bacteria, mesophilic, Gram-negative, oval-shaped

description: Acinetobacter seifertii Gerner-Smidt 10090 is a mesophilic, Gram-negative, oval-shaped bacterium that was isolated from ulcer .

NCBI tax id

  • NCBI tax id: 1530123
  • Matching level: species

strain history

@refhistory
364652012, A. Nemec, Inst. Pasteur, Paris, France: strain NIPH 973
23988<- CCM; CCM 8535 <- A. Nemec; NIPH 973 <- P. Gerner-Smidt; Gerner-Smidt 10090
122551CIP <- 2012, A. Nemec, Inst. Pasteur, Paris, France: strain NIPH 973

doi: 10.13145/bacdive132199.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Moraxellaceae
  • genus: Acinetobacter
  • species: Acinetobacter seifertii
  • full scientific name: Acinetobacter seifertii Nemec et al. 2015

@ref: 23988

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Moraxellaceae

genus: Acinetobacter

species: Acinetobacter seifertii

full scientific name: Acinetobacter seifertii Nemec et al. 2015

strain designation: Gerner-Smidt 10090

type strain: yes

Morphology

cell morphology

  • @ref: 122551
  • gram stain: negative
  • cell shape: oval-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
36465MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
23988TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yesName: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled waterhttps://mediadive.dsmz.de/medium/535
23988COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yesName: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar basehttps://mediadive.dsmz.de/medium/693
122551CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
36465positivegrowth30mesophilic
52318nogrowth44thermophilic
23988positivegrowth37mesophilic

Physiology and metabolism

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate-assimilation
6836927689decanoate+assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose+fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369cytochrome oxidase-1.9.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
23988--+------------+-+++-
23988--+-----+/------+/-+-+++-

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentisolation date
23988ulcer (human patient)DenmarkDNKEurope
52318Human ulcerDenmarkDNKEurope
122551Human, UlcerDenmarkDNKEurope1990

isolation source categories

Cat1Cat2
#Infection#Inflammation
#Infection#Patient
#Host#Human

taxonmaps

  • @ref: 69479
  • File name: preview.99_95.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_8;96_11;97_38;98_86;99_95&stattab=map
  • Last taxonomy: Acinetobacter
  • 16S sequence: FJ860878
  • Sequence Identity:
  • Total samples: 46266
  • soil counts: 7889
  • aquatic counts: 12125
  • animal counts: 20278
  • plant counts: 5974

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
239882Risk group (German classification)
1225511Risk group (French classification)

Sequence information

16S sequences

  • @ref: 23988
  • description: Acinetobacter seifertii strain LUH 1472 16S ribosomal RNA gene, partial sequence
  • accession: FJ860878
  • length: 1460
  • database: ena
  • NCBI tax ID: 1530123

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Acinetobacter seifertii NIPH 973GCA_000368065scaffoldncbi1530123
66792Acinetobacter sp. NIPH 973520709.3wgspatric1530123
66792Acinetobacter seifertii NIPH 9732565956690draftimg1530123

GC content

  • @ref: 23988
  • GC-content: 38.3
  • method: sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno89.578no
flagellatedno94.125no
gram-positiveno97.329no
anaerobicno98.655no
aerobicyes93.325no
halophileno83.012no
spore-formingno96.301no
thermophileno99.936no
glucose-utilno62.992no
glucose-fermentno79.482no

External links

@ref: 23988

culture collection no.: DSM 102854, CCM 8535, CCUG 34785, CIP 110471, NIPH 973, NIPH 973 (

straininfo link

  • @ref: 90553
  • straininfo: 51010

literature

  • topic: Phylogeny
  • Pubmed-ID: 25563912
  • title: Acinetobacter seifertii sp. nov., a member of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex isolated from human clinical specimens.
  • authors: Nemec A, Krizova L, Maixnerova M, Sedo O, Brisse S, Higgins PG
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.000043
  • year: 2015
  • mesh: Acinetobacter/*classification/genetics/isolation & purification, Acinetobacter Infections/microbiology, Bacterial Typing Techniques, DNA, Bacterial/genetics, Genes, Bacterial, Genotype, Humans, Molecular Sequence Data, Multilocus Sequence Typing, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • topic2: Genetics

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
23988Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-102854Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 102854)
36465Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/8333
52318Curators of the CCUGhttps://www.ccug.se/strain?id=34785Culture Collection University of Gothenburg (CCUG) (CCUG 34785)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68369Automatically annotated from API 20NE
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
90553Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID51010.1
122551Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20110471Collection of Institut Pasteur (CIP 110471)