Strain identifier

BacDive ID: 132192

Type strain: Yes

Species: Acinetobacter bohemicus

Strain history: CIP <- 2012, A. Nemec, Inst. Pasteur, Paris, France: strain ANC 3994

NCBI tax ID(s): 1217715 (strain), 1435036 (species)

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General

@ref: 23981

BacDive-ID: 132192

DSM-Number: 102855

keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, oval-shaped

description: Acinetobacter bohemicus DSM 102855 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from wetland soil .

NCBI tax id

NCBI tax idMatching level
1217715strain
1435036species

strain history

@refhistory
23981<- CCM <- A. Nemec; ANC 3994
116832CIP <- 2012, A. Nemec, Inst. Pasteur, Paris, France: strain ANC 3994

doi: 10.13145/bacdive132192.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Moraxellaceae
  • genus: Acinetobacter
  • species: Acinetobacter bohemicus
  • full scientific name: Acinetobacter bohemicus Krizova et al. 2015

  • synonyms

    • @ref: 20215
    • synonym: Acinetobacter pakistanensis

@ref: 23981

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Moraxellaceae

genus: Acinetobacter

species: Acinetobacter bohemicus

full scientific name: Acinetobacter bohemicus Krizova et al. 2015

type strain: yes

Morphology

cell morphology

  • @ref: 116832
  • gram stain: negative
  • cell shape: oval-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
23981TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
23981COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base

culture temp

@refgrowthtypetemperaturerange
63039positivegrowth30mesophilic
23981positivegrowth30mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 63039
  • oxygen tolerance: aerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate-assimilation
6836927689decanoate+assimilation
6836924265gluconate-assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose-assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369cytochrome oxidase-1.9.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
23981---------------+-+---

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudesampling dateisolation date
23981wetland soil (deciduous forest)KrivoklátskoCzech RepublicCZEEurope50.105713.9444
63039Mud,deciduos forrestLány ForestlandCzech RepublicCZEEurope2011-05-19
116832Environment, Forest mudLany forestlandCzech RepublicCZEEurope2011

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Forest
#Environmental#Terrestrial#Soil
#Environmental#Terrestrial#Wetland (Swamp)

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
239811Risk group (German classification)
1168321Risk group (French classification)

Sequence information

16S sequences

  • @ref: 23981
  • description: Acinetobacter bohemicus ANC 3994 16S ribosomal RNA gene, partial sequence
  • accession: KF679797
  • length: 1490
  • database: ena
  • NCBI tax ID: 1217715

GC content

  • @ref: 23981
  • GC-content: 39.6
  • method: sequence analysis

External links

@ref: 23981

culture collection no.: DSM 102855, CCM 8462, CCUG 63842, CIP 110496, ANC 3994

straininfo link

  • @ref: 90546
  • straininfo: 396423

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny25074407Acinetobacter bohemicus sp. nov. widespread in natural soil and water ecosystems in the Czech Republic.Krizova L, Maixnerova M, Sedo O, Nemec ASyst Appl Microbiol10.1016/j.syapm.2014.07.0012014Acinetobacter/chemistry/*classification/genetics/*isolation & purification, Bacterial Typing Techniques, Cluster Analysis, Czech Republic, DNA Gyrase/genetics, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, DNA-Directed RNA Polymerases/genetics, Ecosystem, Histidine/metabolism, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Temperature, *Water MicrobiologyGenetics
Phylogeny25225259Acinetobacter gandensis sp. nov. isolated from horse and cattle.Smet A, Cools P, Krizova L, Maixnerova M, Sedo O, Haesebrouck F, Kempf M, Nemec A, Vaneechoutte MInt J Syst Evol Microbiol10.1099/ijs.0.068791-02014Acinetobacter/*classification/genetics/isolation & purification, Animals, Bacterial Typing Techniques, Base Composition, Belgium, Cattle/*microbiology, DNA, Bacterial/genetics, Fatty Acids/chemistry, Feces/microbiology, Genes, Bacterial, Horses/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNAGenetics
Phylogeny27692032Acinetobacter pakistanensis Abbas et al. 2014 is a later heterotypic synonym of Acinetobacter bohemicus Krizova et al. 2014.Nemec A, Radolfova-Krizova LInt J Syst Evol Microbiol10.1099/ijsem.0.0015302016Acinetobacter/*classification, Bacterial Typing Techniques, DNA, Bacterial/genetics, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Spectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationTranscriptome
33917630Laurus nobilis, Salvia sclarea and Salvia officinalis Essential Oils and Hydrolates: Evaluation of Liquid and Vapor Phase Chemical Composition and Biological Activities.Ovidi E, Laghezza Masci V, Zambelli M, Tiezzi A, Vitalini S, Garzoli SPlants (Basel)10.3390/plants100407072021
34441545Headspace/GC-MS Analysis and Investigation of Antibacterial, Antioxidant and Cytotoxic Activity of Essential Oils and Hydrolates from Rosmarinus officinalis L. and Lavandula angustifolia Miller.Garzoli S, Laghezza Masci V, Franceschi S, Tiezzi A, Giacomello P, Ovidi EFoods10.3390/foods100817682021

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
23981Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-102855Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 102855)
63039Curators of the CCUGhttps://www.ccug.se/strain?id=63842Culture Collection University of Gothenburg (CCUG) (CCUG 63842)
68369Automatically annotated from API 20NE
90546Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID396423.1
116832Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20110496Collection of Institut Pasteur (CIP 110496)