Strain identifier

BacDive ID: 132026

Type strain: Yes

Species: Micromonospora terminaliae

Strain history: <- O. Kaewkla, Flinders Univ., Department of Medical Biotechnology, Adelaide, South Australia; TMS7

NCBI tax ID(s): 1914461 (species)

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General

@ref: 23817

BacDive-ID: 132026

DSM-Number: 101760

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, Gram-positive

description: Micromonospora terminaliae DSM 101760 is an aerobe, spore-forming, Gram-positive bacterium that builds a substrate mycelium and was isolated from Surface sterilized stem of Thai medicinal plant Terminalia mucronata.

NCBI tax id

  • NCBI tax id: 1914461
  • Matching level: species

strain history

  • @ref: 23817
  • history: <- O. Kaewkla, Flinders Univ., Department of Medical Biotechnology, Adelaide, South Australia; TMS7

doi: 10.13145/bacdive132026.20250331.9.3

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micromonosporales
  • family: Micromonosporaceae
  • genus: Micromonospora
  • species: Micromonospora terminaliae
  • full scientific name: Micromonospora terminaliae Kaewkla et al. 2017

@ref: 23817

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Micromonosporaceae

genus: Micromonospora

species: Micromonospora terminaliae

full scientific name: Micromonospora terminaliae Kaewkla et al. 2017

type strain: yes

Morphology

cell morphology

@refgram stainconfidence
25196positive
125439positive99.8
125438positive92.269

multicellular morphology

  • @ref: 25196
  • forms multicellular complex: yes
  • complex name: substrate mycelium
  • further description: mycelium is extensively branched and forms single spores

multimedia

  • @ref: 23817
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_101760.jpg
  • caption: Medium 554 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
23817GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium65.pdf
25196nutrient agar (NA)yes
25196half-strength potato dextrose agar (HPDA)yes
25196Bennett’s agaryes
23817N-Z-AMINE-MEDIUM (DSMZ Medium 554)yeshttps://mediadive.dsmz.de/medium/554Name: N-Z-AMINE-MEDIUM (DSMZ Medium 554) Composition: Starch 20.0 g/l Agar 20.0 g/l Glucose 10.0 g/l N-Z amine 5.0 g/l Yeast extract 5.0 g/l CaCO3 1.0 g/l Distilled water

culture temp

@refgrowthtypetemperature
25196positivegrowth15-45
25196positiveoptimum27-37
23817positivegrowth28

culture pH

  • @ref: 25196
  • ability: positive
  • type: growth
  • pH: 6.0-10.0
  • PH range: alkaliphile

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
25196aerobe
125439obligate aerobe99.7

spore formation

@refspore descriptiontype of sporespore formationconfidence
25196single spores with smooth surfacessporeyes
125438yes93.586

halophily

@refsaltgrowthtested relationconcentration
25196NaClpositivegrowth1 %(w/v)
25196NaClnogrowth5 %(w/v)
25196NaClnogrowth10 %(w/v)
25196NaClnogrowth15 %(w/v)
25196NaClnogrowth20 %(w/v)
25196NaClpositivegrowth3 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2519616716benzene-assimilation
2519616150benzoate-assimilation
2519662968cellulose-builds acid from
2519616947citrate-assimilation
2519617113erythritol-builds acid from
2519617268myo-inositol-builds acid from
2519630924L-tartrate-assimilation
2519624996lactate-builds acid from
2519629864mannitol-builds acid from
2519637657methyl D-glucoside-builds acid from
2519615882phenol-carbon source
2519616634raffinose-builds acid from
2519633942ribose-builds acid from
25196skimmed milk-degradation
2519630911sorbitol-builds acid from
2519617578toluene-assimilation
2519627082trehalose-builds acid from
2519616199urea-degradation
2519627226uric acid-degradation
2519630089acetate+assimilation
251964853esculin+hydrolysis
2519628757fructose+builds acid from
2519628260galactose+builds acid from
2519617234glucose+builds acid from
25196606565hippurate+degradation
2519630849L-arabinose+builds acid from
2519617895L-tyrosine+degradation
2519625115malate+assimilation
2519617306maltose+builds acid from
2519637684mannose+builds acid from
2519617272propionate+assimilation
2519616227pyridine+assimilation
2519628017starch+degradation
2519617992sucrose+builds acid from
2519618222xylose+builds acid from

enzymes

  • @ref: 25196
  • value: catalase
  • activity: +
  • ec: 1.11.1.6

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    25196C15:0 anteiso3.9
    25196C17:0 anteiso5.9
    25196C17:1 anteiso1.3
    25196C14:00.1
    25196C15:00.3
    25196C16:00.6
    25196C16:0 9-methyl10.5
    25196C17:01.8
    25196C17:0 10-methyl1.6
    25196C17:1cis92.7
    25196C18:00.4
    25196C18:0 10-methyl0.2
    25196C18:1cis90.6
    25196C13:0 iso0.3
    25196C14:0 iso1.2
    25196C15:0 iso32.5
    25196C16:0 iso24.4
    25196C16:1 iso H1.6
    25196iso-C16:1cis90.2
    25196C17:0 iso9.1
    25196C18:0 iso0.4
  • type of FA analysis: whole cell analysis
  • incubation medium: tryptic soya broth (Oxoid)
  • agar/liquid: liquid
  • incubation temperature: 30
  • incubation time: 7
  • software version: Sherlock 6.2B
  • library/peak naming table: ACTIN 6
  • system: MIS MIDI
  • method/protocol: Sasser 2001

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment cultureenrichment culture compositionenrichment culture durationenrichment culture temperature
23817Surface sterilized stem of Thai medicinal plant Terminalia mucronataTerminalia mucronataPhujongnayoi district, Ubonratchatani ProvinceThailandTHAAsia
25196stem samples of a Thai medicinal plantPhujongnayoi district, Ubonratchatani ProvinceThailandTHAAsia14105VL70 mediumcontaining a defined amino acid mixture, 0.8 % gellan gum5 weeks37

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Tree
#Host Body-Site#Plant#Stem (Branch)
#Host Body-Site#Plant#Sterilized plant part

Safety information

risk assessment

  • @ref: 23817
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 23817
  • description: Micromonospora terminaliae strain TMS7 16S ribosomal RNA gene, partial sequence
  • accession: KX394339
  • length: 1409
  • database: nuccore
  • NCBI tax ID: 1914461

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Micromonospora terminaliae DSM 101760GCA_009671205completencbi1914461
66792Micromonospora terminaliae strain DSM 1017601914461.3completepatric1914461
66792Micromonospora terminaliae DSM 1017602888122254completeimg1914461

GC content

  • @ref: 23817
  • GC-content: 71.1
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

@refmodeltraitdescriptionpredictionconfidencetraining_data
125438gram-positivegram-positivePositive reaction to Gram-stainingyes92.269no
125438anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no96.822yes
125438aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes89.831no
125438spore-formingspore-formingAbility to form endo- or exosporesyes93.586yes
125438thermophilethermophilicAbility to grow at temperatures above or equal to 45°Cno95yes
125438motile2+flagellatedAbility to perform flagellated movementno89no
125439BacteriaNetspore_formationAbility to form endo- or exosporesyes79.9
125439BacteriaNetmotilityAbility to perform movementno85.2
125439BacteriaNetgram_stainReaction to gram-stainingpositive99.8
125439BacteriaNetoxygen_toleranceOxygenic conditions needed for growthobligate aerobe99.7

External links

@ref: 23817

culture collection no.: DSM 101760, NRRL B-65345, TMS 7

straininfo link

  • @ref: 90399
  • straininfo: 397144

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny28230521Micromonospora terminaliae sp. nov., an endophytic actinobacterium isolated from the surface-sterilized stem of the medicinal plant Terminalia mucronata.Kaewkla O, Thamchaipinet A, Franco CMMInt J Syst Evol Microbiol10.1099/ijsem.0.0016002017Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Micromonospora/*classification/genetics/isolation & purification, Nucleic Acid Hybridization, *Phylogeny, Plant Stems/*microbiology, Plants, Medicinal/microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Terminalia/*microbiology, Thailand, Vitamin K 2/chemistryTranscriptome
Phylogeny32003591Genome Sequence of Micromonospora terminaliae TMS7(T), a New Endophytic Actinobacterium Isolated from the Medicinal Plant Terminalia mucronata.Cui Y, Lv Y, Song M, Wang S, Hu H, Jahan N, Zhu B, Guo LMol Plant Microbe Interact10.1094/MPMI-12-19-0336-A2020Bacterial Typing Techniques, Base Composition, DNA, Bacterial, *Genome, Bacterial, Micromonospora/*genetics, Nucleic Acid Hybridization, Phylogeny, Plants, Medicinal/microbiology, Sequence Analysis, DNA, Terminalia/*microbiologyGenetics

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
23817Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-101760Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 101760)
25196O. T. Kaewkla, A.,Franco, C. M.Micromonospora terminaliae sp. nov., an endophytic actinobacterium isolated from the surface-sterilized stem of the medicinal plant Terminalia mucronata10.1099/ijsem.0.001600IJSEM 67: 225-230 201728230521
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
90399Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID397144.1
125438Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg OvermannPredicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets10.1101/2024.08.12.607695
125439Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardydeepG: Deep Learning for Genome Sequence Data. R package version 0.3.1https://github.com/GenomeNet/deepG