Strain identifier

BacDive ID: 131989

Type strain: Yes

Species: Streptomyces kronopolitis

Strain Designation: NEAU-ML8

Strain history: <- Chongxi Liu, Northeast Agricultural Univ., Harbin, China; NEAU-ML8

NCBI tax ID(s): 1612435 (species)

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General

@ref: 23781

BacDive-ID: 131989

DSM-Number: 101986

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, Gram-positive

description: Streptomyces kronopolitis NEAU-ML8 is an aerobe, spore-forming, Gram-positive bacterium that was isolated from Millipede .

NCBI tax id

  • NCBI tax id: 1612435
  • Matching level: species

strain history

  • @ref: 23781
  • history: <- Chongxi Liu, Northeast Agricultural Univ., Harbin, China; NEAU-ML8

doi: 10.13145/bacdive131989.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces kronopolitis
  • full scientific name: Streptomyces kronopolitis Liu et al. 2016

@ref: 23781

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces kronopolitis

full scientific name: Streptomyces kronopolitis Liu et al. 2016

strain designation: NEAU-ML8

type strain: yes

Morphology

cell morphology

@refgram stainmotilityconfidence
25078positive
69480no93.311
69480positive100

multimedia

  • @ref: 23781
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_101986.jpg
  • caption: Medium 65 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
23781ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium84.pdf
23781GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
25078positivegrowth12.0-38.0
25078positiveoptimum28mesophilic
23781positivegrowth28mesophilic

culture pH

@refabilitytypepH
25078positivegrowth5.0-8.0
25078positiveoptimum7.0-8.0

Physiology and metabolism

oxygen tolerance

  • @ref: 25078
  • oxygen tolerance: aerobe

spore formation

@refspore descriptionspore formationconfidence
25078smooth and non-motileyes
69481yes100
69480yes100

compound production

  • @ref: 23781
  • compound: phoslactomycins

halophily

  • @ref: 25078
  • salt: NaCl
  • tested relation: maximum
  • concentration: 10 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2507862968cellulose-degradation
250784853esculin-hydrolysis
2507817632nitrate-reduction
2507853424tween 20-hydrolysis
2507853426tween 80-hydrolysis
2507816919creatine+nitrogen source
2507815824D-fructose+carbon source
2507812936D-galactose+carbon source
2507817634D-glucose+carbon source
2507816899D-mannitol+carbon source
2507816988D-ribose+carbon source
2507817924D-sorbitol+carbon source
2507865327D-xylose+carbon source
2507815428glycine+nitrogen source
2507817268myo-inositol+carbon source
2507816977L-alanine+nitrogen source
2507830849L-arabinose+carbon source
2507816467L-arginine+nitrogen source
2507817196L-asparagine+nitrogen source
2507829991L-aspartate+nitrogen source
2507829985L-glutamate+nitrogen source
2507818050L-glutamine+nitrogen source
2507817203L-proline+nitrogen source
2507862345L-rhamnose+carbon source
2507817115L-serine+nitrogen source
2507816857L-threonine+nitrogen source
2507817895L-tyrosine+nitrogen source
2507817716lactose+carbon source
2507817306maltose+carbon source
2507816634raffinose+carbon source
2507828017starch+hydrolysis
2507817992sucrose+carbon source
2507853423tween 40+hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
2507816136hydrogen sulfideno
25078phoslactomycinyes

enzymes

@refvalueactivityec
25078catalase+1.11.1.6
25078urease-3.5.1.5

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment cultureenrichment culture compositionenrichment culture durationenrichment culture temperature
23781Millipede (Kronopolites svenhedind Verhoeff)Kronopolites svenhedindHeilongjiang province, Fenghuang Mountain in Wuchang, HarbinChinaCHNAsia45.75126.683
25078Gauseâ??s synthetic agar no. 1supplemented with cycloheximide (50 mg l-1) and nalidixic acid (20 mg l-1)21 days28

isolation source categories

  • Cat1: #Host
  • Cat2: #Arthropoda

Safety information

risk assessment

  • @ref: 23781
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 23781
  • description: Streptomyces kronopolitis strain NEAU-ML8 16S ribosomal RNA gene, partial sequence
  • accession: KP050495
  • length: 1523
  • database: ena
  • NCBI tax ID: 1612435

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptomyces kronopolitis CGMCC 4.7323GCA_014646275scaffoldncbi1612435
66792Streptomyces kronopolitis strain CGMCC 4.73231612435.3wgspatric1612435

GC content

  • @ref: 23781
  • GC-content: 74.9
  • method: thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
flagellatedno98.296no
gram-positiveyes89.361no
anaerobicno99.003yes
aerobicyes92.197yes
halophileno90.026no
spore-formingyes95.062yes
thermophileno95.787no
glucose-utilyes89.917yes
motileno92.706no
glucose-fermentno92.457no

External links

@ref: 23781

culture collection no.: DSM 101986, CGMCC 4.7323

straininfo link

  • @ref: 90375
  • straininfo: 397514

literature

  • topic: Phylogeny
  • Pubmed-ID: 27670691
  • title: Streptomyces kronopolitis sp. nov., an actinomycete that produces phoslactomycins isolated from a millipede (Kronopolites svenhedind Verhoeff).
  • authors: Liu C, Ye L, Li Y, Jiang S, Liu H, Yan K, Xiang W, Wang X
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001520
  • year: 2016
  • mesh: Animals, Arthropods/*microbiology, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Streptomyces/*classification/genetics/isolation & purification, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
23781Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-101986Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 101986)
25078C. Y. Liu, L.,Li, Y.,Jiang, S.,Liu, H.,Yan, K.,Xiang, W.,Wang, X.Streptomyces kronopolitis sp. nov., an actinomycete that produces phoslactomycins isolated from a millipede (Kronopolites svenhedind Verhoeff)10.1099/ijsem.0.001520IJSEM 66: 5352-5357 201627670691
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
90375Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID397514.1