Strain identifier

BacDive ID: 131912

Type strain: No

Species: Pseudomonas stutzeri

Strain history: <- B. Philipp, Univ. Münster, Germany; Chol1

NCBI tax ID(s): 203192 (species), 316 (species)

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General

@ref: 23704

BacDive-ID: 131912

DSM-Number: 103613

keywords: 16S sequence, Bacteria, mesophilic, human pathogen

description: Pseudomonas stutzeri DSM 103613 is a mesophilic human pathogen that was isolated from Soil sample from meadow.

NCBI tax id

NCBI tax idMatching level
203192species
316species

strain history

  • @ref: 23704
  • history: <- B. Philipp, Univ. Münster, Germany; Chol1

doi: 10.13145/bacdive131912.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas stutzeri
  • full scientific name: Pseudomonas stutzeri (Lehmann and Neumann 1896) Sijderius 1946 (Approved Lists 1980)

  • synonyms

    @refsynonym
    20215Stutzerimonas chloritidismutans
    20215Pseudomonas perfectomarinus
    20215Bacterium stutzeri
    20215Pseudomonas kunmingensis
    20215Pseudomonas chloritidismutans
    20215Stutzerimonas kunmingensis
    20215Stutzerimonas stutzeri
    20215Stutzerimonas perfectomarina
    20215Pseudomonas perfectomarina

@ref: 23704

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas stutzeri

full scientific name: Pseudomonas stutzeri (Lehmann and Neumann 1896) Sijderius 1946

type strain: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
23704MINERAL MEDIUM (BRUNNER) (DSMZ Medium 457)yeshttps://mediadive.dsmz.de/medium/457Name: MINERAL MEDIUM (BRUNNER) (DSMZ Medium 457; with strain-specific modifications) Composition: Na2HPO4 2.44 g/l KH2PO4 1.52 g/l KNO3 1.0 g/l (NH4)2SO4 0.5 g/l MgSO4 x 7 H2O 0.2 g/l CaCl2 x 2 H2O 0.05 g/l EDTA 0.005 g/l FeSO4 x 7 H2O 0.002 g/l H3BO3 0.0003 g/l CoCl2 x 6 H2O 0.0002 g/l ZnSO4 x 7 H2O 0.0001 g/l Na2MoO4 x 2 H2O 3e-05 g/l MnCl2 x 4 H2O 3e-05 g/l NiCl2 x 6 H2O 2e-05 g/l CuCl2 x 2 H2O 1e-05 g/l Distilled water
23704TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water

culture temp

  • @ref: 23704
  • growth: positive
  • type: growth
  • temperature: 28
  • range: mesophilic

Physiology and metabolism

compound production

@refcompound
23704hydratase
23704aldehyde reductase

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate-assimilation
6836927689decanoate+assimilation
6836924265gluconate-assimilation
6836917306maltose+assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose+assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate+reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369cytochrome oxidase+1.9.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
23704+-------+----+-+-++-+
23704+-------+----+-+-++-+

Isolation, sampling and environmental information

isolation

  • @ref: 23704
  • sample type: Soil sample from meadow
  • geographic location: Konstanz, Hockgraben
  • country: Germany
  • origin.country: DEU
  • continent: Europe

isolation source categories

Cat1Cat2Cat3
#Engineered#Agriculture#Meadow
#Environmental#Terrestrial#Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_5087.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_23;96_61;97_64;98_148;99_5087&stattab=map
  • Last taxonomy: Pseudomonas
  • 16S sequence: AY727863
  • Sequence Identity:
  • Total samples: 1328
  • soil counts: 220
  • aquatic counts: 520
  • animal counts: 534
  • plant counts: 54

Safety information

risk assessment

  • @ref: 23704
  • pathogenicity human: yes, in single cases
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 23704
  • description: Pseudomonas stutzeri strain Chol1a 16S ribosomal RNA gene, partial sequence
  • accession: AY727863
  • length: 1423
  • database: ena
  • NCBI tax ID: 316

External links

@ref: 23704

culture collection no.: DSM 103613, Chol 1

straininfo link

  • @ref: 90299
  • straininfo: 407521

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Metabolism29054875A Novel Steroid-Coenzyme A Ligase from Novosphingobium sp. Strain Chol11 Is Essential for an Alternative Degradation Pathway for Bile Salts.Yucel O, Holert J, Ludwig KC, Thierbach S, Philipp BAppl Environ Microbiol10.1128/AEM.01492-172017Bacterial Proteins/*genetics/metabolism, Cholates/*metabolism, Coenzyme A Ligases/*genetics/metabolism, Metabolic Networks and Pathways, Sphingomonadaceae/*genetics/metabolism, Steroids/chemistryEnzymology
Metabolism30680854The 7alpha-hydroxysteroid dehydratase Hsh2 is essential for anaerobic degradation of the steroid skeleton of 7alpha-hydroxyl bile salts in the novel denitrifying bacterium Azoarcus sp. strain Aa7.Yucel O, Borgert SR, Poehlein A, Niermann K, Philipp BEnviron Microbiol10.1111/1462-2920.145082019Anaerobiosis, Azoarcus/enzymology/genetics/*metabolism, Bacterial Proteins/genetics/*metabolism, Bile Acids and Salts/chemistry/*metabolism, Cholates/metabolism, Denitrification, Hydroxysteroid Dehydrogenases/genetics/*metabolism, Hydroxysteroids/metabolism, Rhodocyclaceae/enzymology/genetics/metabolism, Steroids/chemistry/metabolismEnzymology
Metabolism34469190Comparative Analysis of Bile-Salt Degradation in Sphingobium sp. Strain Chol11 and Pseudomonas stutzeri Strain Chol1 Reveals Functional Diversity of Proteobacterial Steroid Degradation Enzymes and Suggests a Novel Pathway for Side Chain Degradation.Feller FM, Richtsmeier P, Wege M, Philipp BAppl Environ Microbiol10.1128/AEM.01453-212021Acyl-CoA Dehydrogenase/*metabolism, Bacterial Proteins/metabolism, Bile Acids and Salts/*metabolism, Mixed Function Oxygenases/*metabolism, *Pseudomonas stutzeri/enzymology/genetics, *Sphingomonadaceae/enzymology/genetics, Steroids/*metabolismEnzymology
Metabolism34683472Investigations on the Degradation of the Bile Salt Cholate via the 9,10-Seco-Pathway Reveals the Formation of a Novel Recalcitrant Steroid Compound by a Side Reaction in Sphingobium sp. Strain Chol11.Feller FM, Eilebrecht S, Nedielkov R, Yucel O, Alvincz J, Salinas G, Ludwig KC, Moller H, Philipp BMicroorganisms10.3390/microorganisms91021462021

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
23704Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-103613Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 103613)
68369Automatically annotated from API 20NE
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
90299Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID407521.1