Strain identifier

BacDive ID: 13187

Type strain: Yes

Species: Pseudomonas saponiphila

Strain Designation: 102 G, 102G

Strain history: CIP <- 2008, DSMZ <- W.F. Guerin, Michingan State Univ., Michigan, USA: strain 102G

NCBI tax ID(s): 556534 (species)

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General

@ref: 3713

BacDive-ID: 13187

DSM-Number: 9751

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic, motile

description: Pseudomonas saponiphila 102 G is an obligate aerobe, mesophilic, motile bacterium of the family Pseudomonadaceae.

NCBI tax id

  • NCBI tax id: 556534
  • Matching level: species

strain history

@refhistory
3713<- W. F. Guerin; 102 G
122486CIP <- 2008, DSMZ <- W.F. Guerin, Michingan State Univ., Michigan, USA: strain 102G

doi: 10.13145/bacdive13187.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas saponiphila
  • full scientific name: Pseudomonas saponiphila Lang et al. 2012

@ref: 3713

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas saponiphila

full scientific name: Pseudomonas saponiphila Lang et al. 2012

strain designation: 102 G, 102G

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480yes98.333
6948099.998negative
122486nonegativerod-shaped

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
3713REACTIVATION WITH TRYPTONE SOYA BROTH (DSMZ Medium 220a)yeshttps://mediadive.dsmz.de/medium/220aName: REACTIVATION WITH TRYPTONE SOYA BROTH (DSMZ Medium 220a) Composition: Casein peptone 17.0 g/l Agar 15.0 g/l NaCl 5.0 g/l Soy peptone 3.0 g/l D(+)-Glucose 2.5 g/l K2HPO4 2.5 g/l Distilled water
3713MINERAL MEDIUM (BRUNNER) (DSMZ Medium 457)yeshttps://mediadive.dsmz.de/medium/457Name: MINERAL MEDIUM (BRUNNER) (DSMZ Medium 457) Composition: Na2HPO4 2.44 g/l KH2PO4 1.52 g/l (NH4)2SO4 0.5 g/l MgSO4 x 7 H2O 0.2 g/l CaCl2 x 2 H2O 0.05 g/l EDTA 0.005 g/l FeSO4 x 7 H2O 0.002 g/l H3BO3 0.0003 g/l CoCl2 x 6 H2O 0.0002 g/l ZnSO4 x 7 H2O 0.0001 g/l MnCl2 x 4 H2O 3e-05 g/l Na2MoO4 x 2 H2O 3e-05 g/l NiCl2 x 6 H2O 2e-05 g/l CuCl2 x 2 H2O 1e-05 g/l Distilled water
37363MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
122486CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
3713positivegrowth28mesophilic
37363positivegrowth30mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 122486
  • oxygen tolerance: obligate aerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.992

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate-assimilation
6836927689decanoate+assimilation
6836924265gluconate+assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine+assimilation
6836916899D-mannitol+assimilation
6836916024D-mannose+assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose+assimilation
683695291gelatin+hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine+hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction
12248617632nitrate-reduction
12248616301nitrite-reduction

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleno
12248635581indoleno

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369cytochrome oxidase+1.9.3.1
68369gelatinase+
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase+3.5.3.6
122486oxidase+
122486catalase+1.11.1.6
122486urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
122486-+++-++-+-++--------

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
3713---+--+-+-+++-++-++++

Isolation, sampling and environmental information

isolation

@refgeographic locationcountryorigin.countrycontinentisolation date
3713MichiganUSAUSANorth America
122486MichiganUnited States of AmericaUSANorth America1995

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
37131Risk group (German classification)
1224861Risk group (French classification)

Sequence information

16S sequences

  • @ref: 3713
  • description: Pseudomonas saponiphila 16S rRNA gene, type strain DSM 9751T
  • accession: FM208264
  • length: 1530
  • database: ena
  • NCBI tax ID: 556534

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Pseudomonas saponiphila DSM 9751GCA_900105185contigncbi556534
66792Pseudomonas saponiphila strain DSM 9751556534.4wgspatric556534
66792Pseudomonas saponiphila DSM 97512667528183draftimg556534

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
gram-positiveno98.233no
anaerobicno98.824no
halophileno91.828no
spore-formingno94.87no
glucose-utilyes94.597yes
thermophileno99.859yes
aerobicyes92.322no
motileyes88.439no
flagellatedyes77.518no
glucose-fermentno88.922yes

External links

@ref: 3713

culture collection no.: DSM 9751, CIP 109856

straininfo link

  • @ref: 82395
  • straininfo: 48690

literature

  • topic: Phylogeny
  • Pubmed-ID: 19771475
  • title: Pseudomonas benzenivorans sp. nov. and Pseudomonas saponiphila sp. nov., represented by xenobiotics degrading type strains.
  • authors: Lang E, Burghartz M, Spring S, Swiderski J, Sproer C
  • journal: Curr Microbiol
  • DOI: 10.1007/s00284-009-9507-7
  • year: 2009
  • mesh: Anti-Bacterial Agents/*metabolism, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/chemistry/metabolism, Molecular Sequence Data, Phylogeny, Pseudomonas/chemistry/*classification/genetics/*metabolism, RNA, Ribosomal, 16S/genetics
  • topic2: Metabolism

Reference

@idauthorscataloguedoi/urltitle
3713Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 9751)https://www.dsmz.de/collection/catalogue/details/culture/DSM-9751
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
37363Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/7648
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
82395Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID48690.1StrainInfo: A central database for resolving microbial strain identifiers
122486Curators of the CIPCollection of Institut Pasteur (CIP 109856)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109856