Strain identifier

BacDive ID: 131823

Type strain: Yes

Species: Mycobacterium houstonense

Strain Designation: MF-89, W5198

Strain history: CIP <- 2003, G. Goolsby, CDC, Atlanta, USA: strain W5198 <- 1997, R.J. Wallace, Univ. Texas Hlth. Cent., Tyler, Texas, USA: strain MF 89, Mycobacterium fortuitum

NCBI tax ID(s): 146021 (species)

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General

@ref: 11973

BacDive-ID: 131823

DSM-Number: 44676

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive

description: Mycobacterium houstonense MF-89 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from facial abscess.

NCBI tax id

  • NCBI tax id: 146021
  • Matching level: species

strain history

@refhistory
11973<- J. M. Brown, CDC <- R. J. Wallace
67770CIP 107828 <-- G. Goolsby W5198 <-- R. J. Wallace Mf-89.
119977CIP <- 2003, G. Goolsby, CDC, Atlanta, USA: strain W5198 <- 1997, R.J. Wallace, Univ. Texas Hlth. Cent., Tyler, Texas, USA: strain MF 89, Mycobacterium fortuitum

doi: 10.13145/bacdive131823.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Mycobacteriaceae
  • genus: Mycobacterium
  • species: Mycobacterium houstonense
  • full scientific name: Mycobacterium houstonense Schinsky et al. 2004

  • synonyms

    • @ref: 20215
    • synonym: Mycolicibacterium houstonense

@ref: 11973

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Mycobacteriaceae

genus: Mycobacterium

species: Mycobacterium houstonense

full scientific name: Mycobacterium houstonense Schinsky et al. 2004 emend. Nouioui et al. 2018

strain designation: MF-89, W5198

type strain: yes

Morphology

cell morphology

@refgram stainmotilityconfidencecell shape
29958positive
69480no91.529
69480positive100
119977norod-shaped

multimedia

@refmultimedia contentcaptionintellectual property rights
11973https://www.dsmz.de/microorganisms/photos/DSM_44676.jpgMedium 645 37°C© Leibniz-Institut DSMZ
11973https://www.dsmz.de/microorganisms/photos/DSM_44676-1.jpg© Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11973MIDDLEBROOK MEDIUM (DSMZ Medium 645)yeshttps://mediadive.dsmz.de/medium/645Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water
36379MEDIUM 55 - for Mycobacteriumyes
119977CIP Medium 55yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=55
119977CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
11973positivegrowth37mesophilic
29958positivegrowth28-42
29958positiveoptimum35mesophilic
36379positivegrowth37mesophilic
57764positivegrowth37-42
67770positivegrowth37mesophilic
119977positivegrowth22-37
119977nogrowth10psychrophilic
119977nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
57764aerobe
119977obligate aerobe

spore formation

@refspore formationconfidence
29958no
69481no98
69480no99.941

halophily

  • @ref: 29958
  • salt: NaCl
  • growth: positive
  • tested relation: optimum
  • concentration: 5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2995828757fructose+carbon source
2995817234glucose+carbon source
2995829864mannitol+carbon source
2995837684mannose+carbon source
2995817268myo-inositol+carbon source
2995830911sorbitol+carbon source
2995827082trehalose+carbon source
2995817632nitrate+reduction
119977606565hippurate-hydrolysis
11997717632nitrate+reduction
11997716301nitrite-reduction
11997717632nitrate-respiration

antibiotic resistance

  • @ref: 119977
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 119977
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
29958arylsulfatase+3.1.6.1
29958catalase+1.11.1.6
29958urease+3.5.1.5
119977oxidase-
119977beta-galactosidase-3.2.1.23
119977gelatinase-
119977amylase-
119977DNase-
119977caseinase-3.4.21.50
119977catalase+1.11.1.6
119977tween esterase+
119977gamma-glutamyltransferase+2.3.2.2
119977lecithinase-
119977lipase-
119977protease-
119977urease+3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119977--++-+----++----+---

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
119977++-++---------------------+----+++--+------------+----------+-----------++-+-+--+--++---+---+++-+--

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
11973facial abscessTexas, HoustonUSAUSANorth America
57764Human facial abscessHoustonUSAUSANorth America
67770Face woundHouston, TXUSAUSANorth America
119977Face woundHouston, TexasUnited States of AmericaUSANorth America1980

isolation source categories

Cat1Cat2Cat3
#Host Body-Site#Other#Abscess
#Host Body-Site#Organ#Skin, Nail, Hair

taxonmaps

  • @ref: 69479
  • File name: preview.99_515.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_115;97_219;98_419;99_515&stattab=map
  • Last taxonomy: Mycolicibacterium
  • 16S sequence: LT223693
  • Sequence Identity:
  • Total samples: 496
  • soil counts: 45
  • aquatic counts: 64
  • animal counts: 50
  • plant counts: 337

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
119732Risk group (German classification)
1199771Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
11973Mycobacterium houstonense strain Ho1as 16S ribosomal RNA gene, partial sequenceAY0125791440ena146021
67770Mycobacterium houstonense partial 16S rRNA gene, strain ATCC 49403LT2236931506ena146021

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mycobacterium houstonense strain type strain: ATCC 49403146021.3wgspatric146021
66792Mycolicibacterium houstonense ATCC 494032775507220draftimg146021
67770Mycolicibacterium houstonense ATCC 49403TGCA_900078665scaffoldncbi146021
66792Mycolicibacterium houstonense DSM 44676GCA_025823315scaffoldncbi146021

GC content

@refGC-contentmethod
1197364
6777064thermal denaturation, midpoint method (Tm)
6777067genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno98no
motileno93.209no
gram-positiveyes90.836no
anaerobicno99.527no
halophileno92.585no
spore-formingno86.712yes
thermophileno99.551yes
glucose-utilyes88.157yes
aerobicyes89.399yes
flagellatedno97.959no
glucose-fermentno90.337no

External links

@ref: 11973

culture collection no.: DSM 44676, ATCC 49403, CDC 80-719, CCUG 47581, JCM 15656, CIP 107828

straininfo link

  • @ref: 90214
  • straininfo: 42454

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny15388725Taxonomic variation in the Mycobacterium fortuitum third biovariant complex: description of Mycobacterium boenickei sp. nov., Mycobacterium houstonense sp. nov., Mycobacterium neworleansense sp. nov. and Mycobacterium brisbanense sp. nov. and recognition of Mycobacterium porcinum from human clinical isolates.Schinsky MF, Morey RE, Steigerwalt AG, Douglas MP, Wilson RW, Floyd MM, Butler WR, Daneshvar MI, Brown-Elliott BA, Wallace RJ, McNeil MM, Brenner DJ, Brown JMInt J Syst Evol Microbiol10.1099/ijs.0.02743-02004Anti-Bacterial Agents/pharmacology, Bacterial Typing Techniques, Base Composition, Chaperonin 60/genetics, DNA Fingerprinting, DNA, Bacterial/chemistry/isolation & purification, DNA, Ribosomal/chemistry/isolation & purification, Fatty Acids/analysis, Genes, rRNA/genetics, Humans, Microbial Sensitivity Tests, Molecular Sequence Data, Mycobacterium Infections, Nontuberculous/*microbiology, Mycobacterium fortuitum/*classification/genetics/isolation & purification/physiology, Mycolic Acids/analysis, Nontuberculous Mycobacteria/*classification/genetics/isolation & purification/physiology, Nucleic Acid Hybridization, Phylogeny, Polymorphism, Restriction Fragment Length, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Ribotyping, Sequence Analysis, DNAEnzymology
Genetics27231371Draft Genome Sequence of Mycobacterium houstonense Strain ATCC 49403T.Levasseur A, Asmar S, Robert C, Drancourt MGenome Announc10.1128/genomeA.00443-162016

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
11973Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44676)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44676
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
29958Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2632728776041
36379Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5382
57764Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 47581)https://www.ccug.se/strain?id=47581
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
90214Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID42454.1StrainInfo: A central database for resolving microbial strain identifiers
119977Curators of the CIPCollection of Institut Pasteur (CIP 107828)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107828