Strain identifier

BacDive ID: 13176

Type strain: Yes

Species: Halopseudomonas pelagia

Strain Designation: CL-AP6

Strain history: B. C. Cho CL-AP6.

NCBI tax ID(s): 553151 (species)

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General

@ref: 17924

BacDive-ID: 13176

DSM-Number: 25163

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Halopseudomonas pelagia CL-AP6 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from antarctic green alga Pyramimonas gelidicola.

NCBI tax id

  • NCBI tax id: 553151
  • Matching level: species

strain history

@refhistory
17924<- JCM <- B. C. Cho, Research Institute of Oceanography, Seoul National University; CL-AP6
67770B. C. Cho CL-AP6.

doi: 10.13145/bacdive13176.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Halopseudomonas
  • species: Halopseudomonas pelagia
  • full scientific name: Halopseudomonas pelagia (Hwang et al. 2009) Rudra and Gupta 2021
  • synonyms

    @refsynonym
    20215Neopseudomonas pelagia
    20215Pseudomonas pelagia

@ref: 17924

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas pelagia

full scientific name: Pseudomonas pelagia Hwang et al. 2009

strain designation: CL-AP6

type strain: yes

Morphology

cell morphology

  • @ref: 29181
  • gram stain: negative
  • cell length: 1.9 µm
  • cell width: 0.45 µm
  • cell shape: rod-shaped
  • motility: yes

pigmentation

  • @ref: 29181
  • production: no

multimedia

  • @ref: 17924
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_25163.jpg
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

  • @ref: 17924
  • name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/514
  • composition: Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
17924positivegrowth25mesophilic
29181positivegrowth04-33
29181positiveoptimum25mesophilic
67770positivegrowth28mesophilic

culture pH

@refabilitytypepHPH range
29181positivegrowth6.5-9.1alkaliphile
29181positiveoptimum7.5-8.1

Physiology and metabolism

oxygen tolerance

  • @ref: 29181
  • oxygen tolerance: aerobe

spore formation

  • @ref: 29181
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
29181NaClpositivegrowth0.5-8 %
29181NaClpositiveoptimum01-02 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2918117234glucose+carbon source
2918125017leucine+carbon source
2918115361pyruvate+carbon source
2918153424tween 20+carbon source
2918153423tween 40+carbon source
2918153425tween 60+carbon source
2918117632nitrate+reduction

enzymes

@refvalueactivityec
29181acid phosphatase+3.1.3.2
29181alkaline phosphatase+3.1.3.1

Isolation, sampling and environmental information

isolation

@refsample typehost speciescontinent
17924antarctic green alga Pyramimonas gelidicolaPyramimonas gelidicolaAustralia and Oceania
67770Culture of the Antarctic green alga, Pyramimonas gelidicolaPyramimonas gelidicola

isolation source categories

Cat1Cat2Cat3
#Host#Algae#Green algae
#Condition#Psychrophilic (<10°C)
#Climate#Cold#Polar

Safety information

risk assessment

  • @ref: 17924
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
29181Pseudomonas pelagia strain CL-AP6 16S ribosomal RNA gene, partial sequenceEU8889111460nuccore553151
17924EU88891ena

Genome sequences

  • @ref: 67770
  • description: Halopseudomonas pelagia CL-AP6
  • accession: GCA_000410875
  • assembly level: contig
  • database: ncbi
  • NCBI tax ID: 1323663

GC content

@refGC-contentmethod
1792459.1
6777059.1high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes90.691yes
flagellatedyes92.607no
gram-positiveno98.449no
anaerobicno97.385yes
aerobicyes92.99no
halophileyes57.41no
spore-formingno96.217yes
thermophileno99.748yes
glucose-utilyes50.91yes
glucose-fermentno89.83no

External links

@ref: 17924

culture collection no.: DSM 25163, JCM 15562, KCCM 90073

straininfo link

  • @ref: 82384
  • straininfo: 297192

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmedjournal
17924Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 25163)https://www.dsmz.de/collection/catalogue/details/culture/DSM-25163
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
29181Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2560528776041
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updates33211880Nucleic Acids Res. 49: D498-D508 2020
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
82384Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID297192.1StrainInfo: A central database for resolving microbial strain identifiers