General

@ref: 6816

BacDive-ID: 131756

DSM-Number: 17206

keywords: 16S sequence, Archaea, anaerobe, mesophilic, Gram-variable, rod-shaped

description: Methanothrix harundinacea 8Ac is an anaerobe, mesophilic, Gram-variable archaeon that was isolated from UASB reactor treating beer manufacture wastewater.

NCBI tax id

• NCBI tax id: 301375
• Matching level: species

strain history

• @ref: 6816
• history: <- K. Ma and X. Dong, Inst. Microbiol., Chinese Acad. Sci., Beijing, China; 8Ac <- K. Ma and X. Liu {2004}

doi: 10.13145/bacdive131756.20230509.8.1

Name and taxonomic classification

LPSN

• @ref: 20215
• description: domain/archaea
• keyword: phylum/methanobacteriota
• domain: Archaea
• phylum: Methanobacteriota
• class: Methanomicrobia
• order: Methanosarcinales
• family: Methanotrichaceae
• genus: Methanothrix
• species: Methanothrix harundinacea
• full scientific name: Methanothrix harundinacea (Ma et al. 2006) Akinyemi et al. 2021

• synonyms

  • @ref: 20215
  • synonym: Methanosaeta harundinacea

@ref: 6816

domain: Archaea

phylum: Euryarchaeota

class: Methanomicrobia

order: Methanosarcinales

family: Methanosaetaceae

genus: Methanosaeta

species: Methanosaeta harundinacea

full scientific name: Methanosaeta harundinacea Ma et al. 2006

strain designation: 8Ac

type strain: yes

Morphology

cell morphology

• @ref: 31607
• gram stain: variable
• cell length: 03-05 µm
• cell width: 0.8-1 µm
• cell shape: rod-shaped
• motility: no

pigmentation

• @ref: 31607
• production: yes

Culture and growth conditions

culture medium

• @ref: 6816
• name: METHANOTHRIX MEDIUM (DSMZ Medium 334)
• growth: yes
• link: https://mediadive.dsmz.de/medium/334
• composition: Name: METHANOTHRIX MEDIUM (DSMZ Medium 334; with strain-specific modifications) Composition: Na-acetate 6.75273 g/l Na2CO3 1.24131 g/l Na2HPO4 x 2 H2O 0.854022 g/l Na2S x 9 H2O 0.496524 g/l Yeast extract 0.496524 g/l Trypticase peptone 0.496524 g/l NaCl 0.297915 g/l NH4Cl 0.297915 g/l KH2PO4 0.21847 g/l Nitrilotriacetic acid 0.12711 g/l CaCl2 x 2 H2O 0.109235 g/l MgCl2 x 6 H2O 0.0993049 g/l FeCl3 x 6 H2O 0.0134062 g/l NiCl2 x 6 H2O 0.00119166 g/l MnCl2 x 4 H2O 0.000993049 g/l ZnCl2 0.000993049 g/l Na2-EDTA 0.000993044 g/l Sodium resazurin 0.000496524 g/l Na2SeO3 x 5 H2O 0.000297915 g/l CoCl2 x 6 H2O 0.000297915 g/l Na2MoO4 x 2 H2O 0.000297915 g/l CuCl2 0.000297915 g/l H3BO3 9.93049e-05 g/l Pyridoxine hydrochloride 9.93049e-05 g/l Riboflavin 4.96524e-05 g/l Nicotinic acid 4.96524e-05 g/l Calcium D-(+)-pantothenate 4.96524e-05 g/l (DL)-alpha-Lipoic acid 4.96524e-05 g/l p-Aminobenzoic acid 4.96524e-05 g/l Thiamine HCl 4.96524e-05 g/l Biotin 1.9861e-05 g/l Folic acid 1.9861e-05 g/l Vitamin B12 9.93049e-07 g/l Distilled water

culture temp

culture pH

Physiology and metabolism

oxygen tolerance

• @ref: 31607
• oxygen tolerance: anaerobe

observation

• @ref: 31607
• observation: aggregates in chains

metabolite utilization

• @ref: 31607
• Chebi-ID: 30089
• metabolite: acetate
• utilization activity: +
• kind of utilization tested: carbon source

Isolation, sampling and environmental information

isolation

• @ref: 6816
• sample type: UASB reactor treating beer manufacture wastewater
• geographic location: Beijing
• country: China
• origin.country: CHN
• continent: Asia

isolation source categories

Safety information

risk assessment

• @ref: 6816
• biosafety level: 1
• biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

• @ref: 6816
• description: Methanosaeta harundinacea strain 8Ac 16S ribosomal RNA gene, partial sequence
• accession: AY817738
• length: 1350
• database: ena
• NCBI tax ID: 1127228

GC content

External links

@ref: 6816

culture collection no.: DSM 17206, CGMCC 1.5026, NBRC 104789

straininfo link

• @ref: 90148
• straininfo: 289867

Reference