Strain identifier
BacDive ID: 13161
Type strain: 
Species: Pseudomonas guineae
Strain Designation: M8
Strain history: <- M. J. Montes, Facultat de Farmacia, Univ. Barcelona; M8
NCBI tax ID(s): 425504 (species)
version 8.1 (current version)
General
@ref: 15604
BacDive-ID: 13161
DSM-Number: 21282
keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped
description: Pseudomonas guineae M8 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from soil.
NCBI tax id
- NCBI tax id: 425504
- Matching level: species
strain history
- @ref: 15604
- history: <- M. J. Montes, Facultat de Farmacia, Univ. Barcelona; M8
doi: 10.13145/bacdive13161.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Pseudomonadales
- family: Pseudomonadaceae
- genus: Pseudomonas
- species: Pseudomonas guineae
- full scientific name: Pseudomonas guineae Bozal et al. 2007
@ref: 15604
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Pseudomonadales
family: Pseudomonadaceae
genus: Pseudomonas
species: Pseudomonas guineae
full scientific name: Pseudomonas guineae Bozal et al. 2007
strain designation: M8
type strain: yes
Morphology
cell morphology
| @ref | gram stain | cell length | cell width | cell shape | motility | confidence |
|---|---|---|---|---|---|---|
| 32216 | negative | 1.75 µm | 0.45 µm | rod-shaped | yes | |
| 69480 | yes | 98.712 | ||||
| 69480 | negative | 99.999 |
pigmentation
- @ref: 32216
- production: no
Culture and growth conditions
culture medium
- @ref: 15604
- name: REACTIVATION WITH LIQUID MEDIUM (DSMZ Medium 535b)
- growth: yes
- link: https://mediadive.dsmz.de/medium/535b
- composition: Name: REACTIVATION WITH LIQUID MEDIUM (DSMZ Medium 535b) Composition: Trypticase soy broth 30.0 g/l Casein peptone 17.0 g/l Agar 15.0 g/l NaCl 5.0 g/l Soy peptone 3.0 g/l K2HPO4 2.5 g/l D(+)-Glucose 2.5 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 15604 | positive | growth | 25 | mesophilic |
| 32216 | positive | optimum | 13 | psychrophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 32216
- oxygen tolerance: aerobe
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 32216 | no | |
| 69481 | no | 100 |
| 69480 | no | 99.997 |
halophily
| @ref | salt | growth | tested relation | concentration |
|---|---|---|---|---|
| 32216 | NaCl | positive | growth | 0-4 % |
| 32216 | NaCl | positive | optimum | 2 % |
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 32216 | 27689 | decanoate | + | carbon source |
| 32216 | 16947 | citrate | + | carbon source |
| 32216 | 25115 | malate | + | carbon source |
| 68369 | 25115 | malate | + | assimilation |
| 68369 | 17128 | adipate | - | assimilation |
| 68369 | 24265 | gluconate | - | assimilation |
| 68369 | 17306 | maltose | - | assimilation |
| 68369 | 59640 | N-acetylglucosamine | - | assimilation |
| 68369 | 16899 | D-mannitol | - | assimilation |
| 68369 | 16024 | D-mannose | - | assimilation |
| 68369 | 30849 | L-arabinose | - | assimilation |
| 68369 | 17634 | D-glucose | - | assimilation |
| 68369 | 5291 | gelatin | - | hydrolysis |
| 68369 | 4853 | esculin | - | hydrolysis |
| 68369 | 16199 | urea | - | hydrolysis |
| 68369 | 29016 | arginine | - | hydrolysis |
| 68369 | 17634 | D-glucose | - | fermentation |
| 68369 | 27897 | tryptophan | - | energy source |
| 68369 | 17632 | nitrate | - | reduction |
metabolite production
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 32216 | acid phosphatase | + | 3.1.3.2 |
| 32216 | alkaline phosphatase | + | 3.1.3.1 |
| 32216 | catalase | + | 1.11.1.6 |
| 32216 | cytochrome oxidase | + | 1.9.3.1 |
| 68369 | cytochrome oxidase | + | 1.9.3.1 |
| 68369 | gelatinase | - | |
| 68369 | beta-glucosidase | - | 3.2.1.21 |
| 68369 | urease | - | 3.5.1.5 |
| 68369 | arginine dihydrolase | - | 3.5.3.6 |
API 20NE
| @ref | NO3 | TRP | GLU_ Ferm | ADH Arg | URE | ESC | GEL | PNPG | GLU_ Assim | ARA | MNE | MAN | NAG | MAL | GNT | CAP | ADI | MLT | CIT | PAC | OX |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 15604 | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | + | + | - | + |
| 15604 | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | + | - | + | + | - | + |
Isolation, sampling and environmental information
isolation
- @ref: 15604
- sample type: soil
- geographic location: Deception Island (South Shetland Islands)
- continent: Australia and Oceania
isolation source categories
- Cat1: #Environmental
- Cat2: #Terrestrial
- Cat3: #Soil
taxonmaps
- @ref: 69479
- File name: preview.99_8559.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_23;96_61;97_1186;98_6253;99_8559&stattab=map
- Last taxonomy: Pseudomonas
- 16S sequence: AM491810
- Sequence Identity:
- Total samples: 6438
- soil counts: 958
- aquatic counts: 4373
- animal counts: 948
- plant counts: 159
Safety information
risk assessment
- @ref: 15604
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 15604
- description: Pseudomonas guineae partial 16S rRNA gene, type strain LMG 24016T
- accession: AM491810
- length: 1489
- database: ena
- NCBI tax ID: 425504
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Pseudomonas guineae LMG 24016 | GCA_900113745 | scaffold | ncbi | 425504 |
| 66792 | Pseudomonas guineae strain LMG 24016 | 425504.4 | wgs | patric | 425504 |
| 66792 | Pseudomonas guineae LMG 24016 | 2667527413 | draft | img | 425504 |
GC content
- @ref: 32216
- GC-content: 58.5
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | no | 100 | no |
| motile | yes | 90.234 | yes |
| flagellated | yes | 91.266 | no |
| gram-positive | no | 98.849 | no |
| anaerobic | no | 97.592 | yes |
| aerobic | yes | 93.369 | yes |
| halophile | no | 59.083 | no |
| spore-forming | no | 96.485 | yes |
| glucose-ferment | no | 89.789 | yes |
| thermophile | no | 99.387 | no |
| glucose-util | yes | 63.975 | yes |
External links
@ref: 15604
culture collection no.: DSM 21282, CECT 7231, LMG 24016
straininfo link
- @ref: 82369
- straininfo: 301339
literature
- topic: Phylogeny
- Pubmed-ID: 17978227
- title: Pseudomonas guineae sp. nov., a novel psychrotolerant bacterium from an Antarctic environment.
- authors: Bozal N, Montes MJ, Mercade E
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.65141-0
- year: 2007
- mesh: Antarctic Regions, Bacterial Typing Techniques, *Cold Temperature, DNA, Ribosomal/analysis, Fatty Acids/analysis, Genes, rRNA, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, Pseudomonas/chemistry/*classification/genetics/isolation & purification, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Species Specificity
- topic2: Genetics
Reference
| @id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed | journal |
|---|---|---|---|---|---|---|---|
| 15604 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21282) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-21282 | ||||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | ||||
| 32216 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 28458 | 28776041 | ||
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | ||||
| 66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | 33211880 | Nucleic Acids Res. 49: D498-D508 2020 | ||
| 68369 | Automatically annotated from API 20NE | ||||||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | ||||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | ||||
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | ||||
| 82369 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID301339.1 | StrainInfo: A central database for resolving microbial strain identifiers |