Strain identifier

BacDive ID: 131529

Type strain: Yes

Species: Muricomes intestini

Strain Designation: 2-PG-424-CC-1

Strain history: <- Barbel Fosel, DSM

NCBI tax ID(s): 1796634 (species)

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General

@ref: 22756

BacDive-ID: 131529

DSM-Number: 29489

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, mesophilic, rod-shaped

description: Muricomes intestini 2-PG-424-CC-1 is an anaerobe, spore-forming, mesophilic bacterium that was isolated from caecal content; TNFdeltaARE/+ C57BL/6 mouse.

NCBI tax id

  • NCBI tax id: 1796634
  • Matching level: species

strain history

@refhistory
22756<- T. Clavel, TU Munich, Freising-Weihenstephan, Germany; 2-PG-424-CC-1
67771<- Barbel Fosel, DSM

doi: 10.13145/bacdive131529.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Lachnospiraceae
  • genus: Muricomes
  • species: Muricomes intestini
  • full scientific name: Muricomes intestini Lagkouvardos et al. 2016

@ref: 22756

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Eubacteriales

family: Lachnospiraceae

genus: Muricomes

species: Muricomes intestini

full scientific name: Muricomes intestini Lagkouvardos et al. 2016

strain designation: 2-PG-424-CC-1

type strain: yes

Morphology

cell morphology

@refcell shapegram stainconfidence
67771rod-shaped
67771positive
69480positive100

colony morphology

  • @ref: 22756
  • incubation period: 2-3 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
22756WILKINS-CHALGREN ANAEROBE BROTH (DSMZ Medium 339)yeshttps://mediadive.dsmz.de/medium/339Name: WILKINS-CHALGREN ANAEROBE BROTH (DSMZ Medium 339) Composition: dehydrated Wilkins-Chalgren medium 33.0 g/l L-Cysteine HCl 0.3 g/l Sodium resazurin 0.0005 g/l Distilled water
22756COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base

culture temp

@refgrowthtypetemperaturerange
22756positivegrowth37mesophilic
67771positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
22756anaerobe
67771anaerobe
69480anaerobe100

spore formation

@refspore formationconfidence
69481yes96
69480no98.807

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838016199urea-hydrolysis
6838029016arginine-hydrolysis
6838016634raffinose-fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan-energy source

metabolite production

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68380serine arylamidase-
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase-
68380glycin arylamidase-
68380alanine arylamidase-3.4.11.2
68380tyrosine arylamidase-
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase-3.4.11.1
68380phenylalanine arylamidase-
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase+3.4.11.5
68380L-arginine arylamidase-
68380alkaline phosphatase-3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase-3.2.1.21
68380alpha-glucosidase+3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase-3.2.1.23
68380alpha-galactosidase-3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
22756-----+----+--+----+----------
22756-----+----+/---+/----+----------
22756-----+-------+/----+----------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
22756caecal content; TNFdeltaARE/+ C57BL/6 mouseFreisingGermanyDEUEurope
67771From mouse instine, caecal content; TNFdeltaARE/+ C57BL/6 mouseFreisingGermanyDEUEurope

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Muridae (Mouse/Rat)
#Host Body-Site#Gastrointestinal tract#Large intestine
#Host Body Product#Gastrointestinal tract#Caecal content

taxonmaps

  • @ref: 69479
  • File name: preview.99_57162.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_16;96_7481;97_8986;98_11017;99_57162&stattab=map
  • Last taxonomy: Muricomes intestini subclade
  • 16S sequence: KR364770
  • Sequence Identity:
  • Total samples: 1077
  • soil counts: 187
  • aquatic counts: 24
  • animal counts: 852
  • plant counts: 14

Safety information

risk assessment

  • @ref: 22756
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 22756
  • description: Muricomes intestini strain 2PG-424-CC-1 16S ribosomal RNA gene, partial sequence
  • accession: KR364770
  • length: 1431
  • database: ena
  • NCBI tax ID: 1796634

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Muricomes intestini DSM 29489GCA_004346165contigncbi1796634
66792Muricomes intestini strain DSM 294891796634.3wgspatric1796634
66792Muricomes intestini DSM 294892784746785draftimg1796634

GC content

@refGC-contentmethod
2275643.1sequence analysis
6777143.1genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes96no
motileyes77.837no
gram-positiveyes85.56yes
anaerobicyes99.31yes
aerobicno98.884yes
halophileno91.825no
spore-formingyes68.306no
glucose-utilyes91.587no
flagellatedno68.858no
thermophileno93.426yes
glucose-fermentyes73.227no

External links

@ref: 22756

culture collection no.: DSM 29489, KCTC 15545

straininfo link

  • @ref: 89943
  • straininfo: 403591

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
22756Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-29489Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 29489)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
68380Automatically annotated from API rID32A
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
89943Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID403591.1