Strain identifier

BacDive ID: 131527

Type strain: Yes

Species: Cuneatibacter caecimuris

Strain Designation: BARN-424-CC-10

Strain history: <- Barbel Fosel, DSM, Germany

NCBI tax ID(s): 1796618 (species)

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General

@ref: 22754

BacDive-ID: 131527

DSM-Number: 29486

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, rod-shaped

description: Cuneatibacter caecimuris BARN-424-CC-10 is a microaerophile, mesophilic, rod-shaped bacterium that was isolated from caecal content; TNFdeltaARE/+ C57BL/6 mouse.

NCBI tax id

  • NCBI tax id: 1796618
  • Matching level: species

strain history

@refhistory
22754<- T. Clavel, TU Munich, Freising-Weihenstephan, Germany; BARN-424-CC-10
67771<- Barbel Fosel, DSM, Germany

doi: 10.13145/bacdive131527.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Lachnospiraceae
  • genus: Cuneatibacter
  • species: Cuneatibacter caecimuris
  • full scientific name: Cuneatibacter caecimuris Lagkouvardos et al. 2016

@ref: 22754

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Eubacteriales

family: Lachnospiraceae

genus: Cuneatibacter

species: Cuneatibacter caecimuris

full scientific name: Cuneatibacter caecimuris Lagkouvardos et al. 2016

strain designation: BARN-424-CC-10

type strain: yes

Morphology

cell morphology

@refcell shapegram stainconfidence
67771rod-shaped
67771positive
69480positive100

colony morphology

  • @ref: 22754
  • incubation period: 2-3 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
22754WILKINS-CHALGREN ANAEROBE BROTH (DSMZ Medium 339)yeshttps://mediadive.dsmz.de/medium/339Name: WILKINS-CHALGREN ANAEROBE BROTH (DSMZ Medium 339) Composition: dehydrated Wilkins-Chalgren medium 33.0 g/l L-Cysteine HCl 0.3 g/l Sodium resazurin 0.0005 g/l Distilled water
22754COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base

culture temp

@refgrowthtypetemperaturerange
22754positivegrowth37mesophilic
67771positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
22754microaerophile
67771anaerobe
69480anaerobe99.344

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 93.237

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838016199urea-hydrolysis
6838029016arginine-hydrolysis
6838016024D-mannose-fermentation
6838016634raffinose-fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan-energy source

metabolite production

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68380serine arylamidase-
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase-
68380glycin arylamidase-
68380alanine arylamidase-3.4.11.2
68380leucine arylamidase-3.4.11.1
68380phenylalanine arylamidase-
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase-3.4.11.5
68380L-arginine arylamidase-
68380alkaline phosphatase-3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase+3.2.1.31
68380alpha-arabinosidase+3.2.1.55
68380beta-glucosidase+3.2.1.21
68380alpha-glucosidase+3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase+3.2.1.23
68380alpha-galactosidase+3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
22754--++-++++--------------------
22754--++-++++--------------+/------
22754--++-++++----+/---------+/-+/------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
22754caecal content; TNFdeltaARE/+ C57BL/6 mouseFreisingGermanyDEUEurope
67771From mouse instine, caecal content; TNFdeltaARE/+ C57BL/6 mouseFreisingGermanyDEUEurope

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Muridae (Mouse/Rat)
#Host Body-Site#Gastrointestinal tract#Large intestine
#Host Body Product#Gastrointestinal tract#Caecal content

taxonmaps

  • @ref: 69479
  • File name: preview.99_118739.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_16;96_46904;97_60246;98_80071;99_118739&stattab=map
  • Last taxonomy: Cuneatibacter caecimuris subclade
  • 16S sequence: KR364760
  • Sequence Identity:
  • Total samples: 22836
  • soil counts: 36
  • aquatic counts: 214
  • animal counts: 22542
  • plant counts: 44

Safety information

risk assessment

  • @ref: 22754
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 22754
  • description: Cuneatibacter caecimuris strain BARN-424-CC-10 16S ribosomal RNA gene, partial sequence
  • accession: KR364760
  • length: 1485
  • database: ena
  • NCBI tax ID: 1796618

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Cuneatibacter caecimuris DSM 29486GCA_004216775scaffoldncbi1796618
66792Cuneatibacter caecimuris strain DSM 294861796618.3wgspatric1796618
66792Cuneatibacter caecimuris DSM 294862802428826draftimg1796618

GC content

@refGC-contentmethod
2275449.1sequence analysis
6777149.1

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes77no
flagellatedno93.339no
gram-positiveyes93.521yes
anaerobicyes98.995no
aerobicno98.409yes
halophileno90.856no
spore-formingyes55.809no
glucose-utilyes91.608no
thermophileno98.446yes
motileno86.126no
glucose-fermentyes76.147no

External links

@ref: 22754

culture collection no.: DSM 29486, KCTC 15539

straininfo link

  • @ref: 89941
  • straininfo: 397974

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
22754Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-29486Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 29486)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar SchomburgBRENDA, the ELIXIR core data resource in 2021: new developments and updates10.1093/nar/gkaa1025Nucleic Acids Res. 49: D498-D508 202033211880
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
68380Automatically annotated from API rID32A
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
89941Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID397974.1