Strain identifier

BacDive ID: 131515

Type strain: Yes

Species: Acutalibacter muris

Strain Designation: KB18

Strain history: <- Barbel Fosel, DSM <- B Stecher, Max von Pettenkofer-Inst., Munich, KB18

NCBI tax ID(s): 1796620 (species)

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General

@ref: 22742

BacDive-ID: 131515

DSM-Number: 26090

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, rod-shaped

description: Acutalibacter muris KB18 is an anaerobe, mesophilic, rod-shaped bacterium that was isolated from feces, SPF TCRMOG92-106/I-As transgenic SJL/J mouse.

NCBI tax id

  • NCBI tax id: 1796620
  • Matching level: species

strain history

@refhistory
22742<- B. Stecher, Max von Pettenkofer-Inst., Munich; KB18
67771<- Barbel Fosel, DSM <- B Stecher, Max von Pettenkofer-Inst., Munich, KB18

doi: 10.13145/bacdive131515.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Oscillospiraceae
  • genus: Acutalibacter
  • species: Acutalibacter muris
  • full scientific name: Acutalibacter muris Lagkouvardos et al. 2016

@ref: 22742

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Oscillospiraceae

genus: Acutalibacter

species: Acutalibacter muris

full scientific name: Acutalibacter muris Lagkouvardos et al. 2016

strain designation: KB18

type strain: yes

Morphology

cell morphology

@refcell shapegram stain
68367rod-shaped
67771positive

colony morphology

  • @ref: 22742
  • incubation period: 3-7 days

Culture and growth conditions

culture medium

  • @ref: 22742
  • name: BIFIDOBACTERIUM MEDIUM (DSMZ Medium 58)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/58
  • composition: Name: BIFIDOBACTERIUM MEDIUM (DSMZ Medium 58) Composition: Glucose 10.0 g/l Casein peptone 10.0 g/l Bacto Soytone 5.0 g/l Meat extract 5.0 g/l Yeast extract 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l MnSO4 x H2O 0.05 g/l KH2PO4 0.04 g/l K2HPO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Tween 80 Resazurin Distilled water

culture temp

@refgrowthtypetemperaturerange
22742positivegrowth37mesophilic
67771positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
22742anaerobe
67771anaerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836727082trehalose-builds acid from
6836762345L-rhamnose-builds acid from
6836730911sorbitol-builds acid from
6836716634raffinose-builds acid from
683676731melezitose-builds acid from
6836716024D-mannose-builds acid from
6836717057cellobiose-builds acid from
6836717754glycerol-builds acid from
683674853esculin+hydrolysis
6836730849L-arabinose-builds acid from
6836765327D-xylose-builds acid from
6836717814salicin-builds acid from
6836717306maltose-builds acid from
6836717992sucrose-builds acid from
6836717716lactose-builds acid from
6836716899D-mannitol-builds acid from
6836717634D-glucose-builds acid from
6836716199urea-hydrolysis
6836727897tryptophan-energy source
6838016199urea-hydrolysis
6838029016arginine-hydrolysis
6838016024D-mannose-fermentation
6838016634raffinose-fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan-energy source

metabolite production

@refChebi-IDmetaboliteproduction
6836735581indoleno
6838035581indoleno

metabolite tests

@refChebi-IDmetaboliteindole test
6838035581indole-
6836735581indole-

enzymes

@refvalueactivityec
68380serine arylamidase-
68380glutamyl-glutamate arylamidase-
68380alanine arylamidase-3.4.11.2
68380phenylalanine arylamidase-
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase-3.4.11.5
68380L-arginine arylamidase-
68380alkaline phosphatase-3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase+3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase+3.2.1.31
68380alpha-arabinosidase+3.2.1.55
68380beta-glucosidase+3.2.1.21
68380beta-galactosidase+3.2.1.23
68380alpha-galactosidase+3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5
68367catalase-1.11.1.6
68367beta-glucosidase+3.2.1.21
68367urease-3.5.1.5

API 20A

@refINDUREGLUMANLACSACMALSALXYLARAGELESCGLYCELMNEMLZRAFSORRHATRECATCOCC
22742----------+/-+----------
22742----------+/-+----------

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
22742--+++/--+++----+-------+-------
22742--++++/-+++----+-------+/--+/--+/-+/---
22742--+++-+++----+-------+/-+------
22742--++++/-+++----+-------+/--+/--+/-+/---

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
22742feces, SPF TCRMOG92-106/I-As transgenic (RR) SJL/J mouseMunichGermanyDEUEurope
67771From feces, SPF TCRMOG92-106/I-As transgenic (RR) SJL/J mouseMunichGermanyDEUEurope

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Muridae (Mouse/Rat)
#Host Body Product#Gastrointestinal tract#Feces (Stool)

taxonmaps

  • @ref: 69479
  • File name: preview.99_1894.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_229;96_1066;97_1244;98_1493;99_1894&stattab=map
  • Last taxonomy: Acutalibacter muris subclade
  • 16S sequence: KR364749
  • Sequence Identity:
  • Total samples: 13668
  • soil counts: 66
  • aquatic counts: 48
  • animal counts: 13538
  • plant counts: 16

Safety information

risk assessment

  • @ref: 22742
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
22742Acutalibacter muris strain KB18 16S ribosomal RNA gene, partial sequenceKR3647491510ena1796620
67771Hungateiclostridiaceae bacterium KB18 chromosome, complete genomeCP0154003802844ena1834198

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Acutalibacter muris KB18GCA_002201475chromosomencbi1796620
66792Acutalibacter muris KB18GCA_016697365chromosomencbi1796620
66792Acutalibacter muris strain KB181796620.3wgspatric1796620
66792Acutalibacter muris KB182757320831completeimg1796620

GC content

@refGC-contentmethod
2274254.6sequence analysis
6777154.6

External links

@ref: 22742

culture collection no.: DSM 26090, KCTC 15540

straininfo link

  • @ref: 89929
  • straininfo: 400889

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
22742Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-26090Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 26090)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
68367Automatically annotated from API 20A
68380Automatically annotated from API rID32A
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
89929Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID400889.1