Strain identifier

BacDive ID: 13145

Type strain: Yes

Species: Pseudomonas mucidolens

Strain Designation: 37

Strain history: CIP <- 1989, NCIB <- ATCC <- W.E. Burns: strain 37 <- M. Levine and D. Anderson

NCBI tax ID(s): 1215111 (strain), 46679 (species)

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General

@ref: 7958

BacDive-ID: 13145

DSM-Number: 19186

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, motile

description: Pseudomonas mucidolens 37 is an aerobe, mesophilic, motile bacterium that was isolated from musty egg.

NCBI tax id

NCBI tax idMatching level
46679species
1215111strain

strain history

@refhistory
7958<- CIP <- NCIB <- ATCC <- W. E. Burns; 37 <- M. Levine and D. Anderson
67770K. Komagata KS 0038 <-- AJ 2213.
121152CIP <- 1989, NCIB <- ATCC <- W.E. Burns: strain 37 <- M. Levine and D. Anderson

doi: 10.13145/bacdive13145.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas mucidolens
  • full scientific name: Pseudomonas mucidolens Levine and Anderson 1932 (Approved Lists 1980)

@ref: 7958

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas mucidolens

full scientific name: Pseudomonas mucidolens Levine and Anderson 1932

strain designation: 37

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480yes97.448
6948099.996negative
121152yesnegativerod-shaped

pigmentation

  • @ref: 121152
  • production: no
  • name: Pyocyanin

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
7958MEDIUM R2A MODIFIED (DSMZ Medium 830a)yeshttps://mediadive.dsmz.de/medium/830aName: MEDIUM R2A MODIFIED (DSMZ Medium 830a) Composition: Agar 15.0 g/l Yeast extract 0.5 g/l Proteose peptone 0.5 g/l Casamino acids 0.5 g/l Glucose 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Tween 80 Distilled water
34241MEDIUM 18 - for Hydrogenophaga, Vogesella and Pseudomonas generayesDistilled water make up to (1000.000 ml);Sodium chloride (3.000 g);Agar(12.000 g);Yeast extract (3.000 g);Peptone (0.600 g)
121152CIP Medium 18yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=18

culture temp

@refgrowthtypetemperaturerange
7958positivegrowth25mesophilic
34241positivegrowth25
44371positivegrowth30
67770positivegrowth30mesophilic
121152positivegrowth10-30
121152nogrowth5psychrophilic
121152nogrowth37mesophilic
121152nogrowth41thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
44371aerobe
121152obligate aerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.921

halophily

@refsaltgrowthtested relationconcentration
121152NaClpositivegrowth0-2 %
121152NaClnogrowth4 %
121152NaClnogrowth6 %
121152NaClnogrowth8 %
121152NaClnogrowth10 %

observation

  • @ref: 67770
  • observation: quinones: Q-9

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose-builds acid from15824
68371D-glucose-builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
68369malate+assimilation25115
68369adipate-assimilation17128
68369decanoate+assimilation27689
68369gluconate+assimilation24265
68369maltose-assimilation17306
68369N-acetylglucosamine+assimilation59640
68369D-mannitol-assimilation16899
68369L-arabinose+assimilation30849
68369D-glucose+assimilation17634
68369gelatin-hydrolysis5291
68369esculin-hydrolysis4853
68369urea-hydrolysis16199
68369arginine-hydrolysis29016
68369D-glucose-fermentation17634
68369tryptophan-energy source27897
68369nitrate+reduction17632
121152citrate+carbon source16947
121152esculin-hydrolysis4853
121152nitrate+reduction17632
121152nitrite-reduction16301
121152phenol-degradation15882
121152nitrate-respiration17632

antibiotic resistance

  • @ref: 121152
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleno
12115235581indoleno

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369cytochrome oxidase+1.9.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6
121152oxidase+
121152beta-galactosidase-3.2.1.23
121152alcohol dehydrogenase-1.1.1.1
121152gelatinase+/-
121152amylase-
121152DNase-
121152caseinase+3.4.21.50
121152catalase+1.11.1.6
121152tween esterase+
121152lecithinase+
121152lipase+
121152lysine decarboxylase-4.1.1.18
121152ornithine decarboxylase-4.1.1.17
121152protease+
121152tryptophan deaminase-
121152urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    44371C12:03.712
    44371C16:023.516
    44371C18:0218
    44371C10:0 3OH5.111.423
    44371C12:0 2OH5.213.178
    44371C12:0 3OH4.513.455
    44371C16:1 ω7c36.815.819
    44371C18:1 ω7c /12t/9t19.217.824
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121152-+++-+----++--------

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
7958+-------+++/--+-++-++-+

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
121152-------------------------------------------------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
121152+-+++-------------++++----+++--+++--++--+--++----++--++++-+++---------++++++++++++-+--+-+++++++++++

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
7958musty eggDes Moines, IowaUSAUSANorth America
121152Food, Musty egg

isolation source categories

Cat1Cat2Cat3
#Environmental#Microbial community
#Host Body Product#Urogenital tract#Egg

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
79581Risk group (German classification)
1211521Risk group (French classification)

Sequence information

16S sequences

  • @ref: 7958
  • description: Pseudomonas mucidolens 16S rRNA gene, complete sequence
  • accession: D84017
  • length: 1526
  • database: ena
  • NCBI tax ID: 46679

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Pseudomonas mucidolens LMG 2223GCA_900106045chromosomencbi46679
66792Pseudomonas mucidolens NCTC8068GCA_900475945completencbi46679
66792Pseudomonas mucidolens NBRC 103159 strain NBRC 1031591215111.3wgspatric1215111
66792Pseudomonas mucidolens strain LMG 222346679.3completepatric46679
66792Pseudomonas mucidolens strain NCTC806846679.5completepatric46679
66792Pseudomonas mucidolens LMG 22232667527233draftimg46679
66792Pseudomonas mucidolens NCTC 80682808606649completeimg46679
67770Pseudomonas mucidolens NBRC 103159GCA_002091735contigncbi1215111
67770Pseudomonas mucidolens NCTC11032GCA_900625055contigncbi46679

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
flagellatedyes77.398no
gram-positiveno98.421no
anaerobicno98.298no
aerobicyes92.033no
halophileno90.99no
spore-formingno94.559no
thermophileno99.844no
glucose-utilyes94.735yes
motileyes88.527no
glucose-fermentno89.47yes

External links

@ref: 7958

culture collection no.: DSM 19186, CIP 103298, CCUG 1424, ATCC 4685, NCTC 8068, JCM 2781, CGMCC 1.1795, IAM 12406, IAM 15111, LMG 2223, NBRC 103159, NCIMB 9394, NRRL B-16

straininfo link

  • @ref: 82352
  • straininfo: 14308

literature

  • topic: Metabolism
  • Pubmed-ID: 16096695
  • title: Regulation of the pyrimidine biosynthetic pathway in Pseudomonas mucidolens.
  • authors: West TP
  • journal: Antonie Van Leeuwenhoek
  • DOI: 10.1007/s10482-005-3871-5
  • year: 2005
  • mesh: Aspartate Carbamoyltransferase/genetics/metabolism, Bacterial Proteins/genetics/metabolism, *Gene Expression Regulation, Bacterial, Pseudomonas/*enzymology/genetics/growth & development, Pyrimidines/*biosynthesis/metabolism
  • topic2: Enzymology

Reference

@idauthorscataloguedoi/urltitle
7958Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 19186)https://www.dsmz.de/collection/catalogue/details/culture/DSM-19186
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
34241Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15080
44371Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 1424)https://www.ccug.se/strain?id=1424
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68369Automatically annotated from API 20NE
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
82352Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID14308.1StrainInfo: A central database for resolving microbial strain identifiers
121152Curators of the CIPCollection of Institut Pasteur (CIP 103298)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103298