Strain identifier
BacDive ID: 131435
Type strain:
Species: Streptomyces lushanensis
Strain history: W.-J. Li JXJ 0135.
NCBI tax ID(s): 1434255 (species)
General
@ref: 22662
BacDive-ID: 131435
DSM-Number: 42121
keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic
description: Streptomyces lushanensis DSM 42121 is a spore-forming, mesophilic bacterium that builds an aerial mycelium and was isolated from soil.
NCBI tax id
- NCBI tax id: 1434255
- Matching level: species
strain history
@ref | history |
---|---|
22662 | <- W.-J. Li, YIM; JXJ 0135 <- B.-H. Zhang |
67770 | W.-J. Li JXJ 0135. |
doi: 10.13145/bacdive131435.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Kitasatosporales
- family: Streptomycetaceae
- genus: Streptomyces
- species: Streptomyces lushanensis
- full scientific name: Streptomyces lushanensis Zhang et al. 2015
@ref: 22662
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Streptomycetaceae
genus: Streptomyces
species: Streptomyces lushanensis
full scientific name: Streptomyces lushanensis Zhang et al. 2015
type strain: yes
Morphology
cell morphology
@ref | motility | confidence | gram stain |
---|---|---|---|
69480 | no | 93.206 | |
69480 | 100 | positive |
colony morphology
@ref | colony color | medium used |
---|---|---|
69347 | Beige (1001), Olive brown (8008) | ISP 5 |
69347 | Beige (1001), Grey beige (1019) | ISP 2 |
69347 | Beige (1001), Grey beige (1019) | suter without tyrosine |
69347 | Beige (1001), Fawn brown (8007) | ISP 7 |
69347 | Beige (1001) | ISP 6 |
69347 | Sand yellow (1002), Fawn brown (8007) | ISP 3 |
69347 | Sand yellow (1002), Light ivory (1015) | suter with tyrosine |
multicellular morphology
@ref | forms multicellular complex | complex name | complex color | medium name |
---|---|---|---|---|
69347 | yes | Aerial mycelium | Signal white (9003) | ISP 2 |
69347 | yes | Aerial mycelium | Traffic white (9016), yellow grey (7034), pebble grey (7032) | ISP 3 |
69347 | yes | Aerial mycelium | Beige (1001), fawn brown (8007) | ISP 4 |
69347 | yes | Aerial mycelium | Traffic white (9016), yellow grey (7034) | ISP 5 |
69347 | no | Aerial mycelium | ISP 6 | |
69347 | yes | Aerial mycelium | Grey white (9002), yellow grey (7034) | ISP 7 |
69347 | yes | Aerial mycelium | Cream (9001) | suter with tyrosine |
69347 | yes | Aerial mycelium | Cream (9001) | suter without tyrosine |
pigmentation
@ref | production | name | color |
---|---|---|---|
69347 | no | Melanin | |
69347 | yes | soluble pigment | Daffodil yellow (1004), brown beige (1011), pebble grey (7032), yellow grey (7034), traffic white (9016), ivory (1014), sand yellow (1002) |
multimedia
@ref | multimedia content | caption | intellectual property rights |
---|---|---|---|
22662 | https://www.dsmz.de/microorganisms/photos/DSM_42121.jpg | Medium 84 28°C | © Leibniz-Institut DSMZ |
69347 | DSM_42121_image3.jpeg | Plates (65, ISP2, ISP3, ISP4, ISP5, ISP7) | Helmholtz-Zentrum für Infektionsforschung GmbH |
69347 | DSM_42121_image4.jpeg | Plates (65, ISP2, ISP3, ISP4, ISP5, ISP7) | Helmholtz-Zentrum für Infektionsforschung GmbH |
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
22662 | GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) | yes | https://mediadive.dsmz.de/medium/65 | Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water |
22662 | ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) | yes | https://mediadive.dsmz.de/medium/84 | Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water |
22662 | STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) | yes | https://mediadive.dsmz.de/medium/252 | Name: STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) Composition: Agar 14.985 g/l Starch 9.99001 g/l (NH4)2SO4 1.998 g/l CaCO3 1.998 g/l K2HPO4 0.999001 g/l MgSO4 x 7 H2O 0.999001 g/l NaCl 0.999001 g/l FeSO4 x 7 H2O 0.000999001 g/l MnCl2 x 4 H2O 0.000999001 g/l ZnSO4 x 7 H2O 0.000999001 g/l Distilled water |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
22662 | positive | growth | 28 | mesophilic |
67770 | positive | growth | 28 | mesophilic |
Physiology and metabolism
spore formation
@ref | spore formation | confidence |
---|---|---|
69481 | yes | 100 |
69480 | yes | 100 |
halophily
- @ref: 69347
- salt: NaCl
- growth: positive
- tested relation: growth
- concentration: 0-10 %
observation
- @ref: 67770
- observation: quinones: MK-9(H6), MK-9(H8)
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
69347 | 22599 | arabinose | - | growth |
69347 | 62968 | cellulose | - | growth |
69347 | 28757 | fructose | + | growth |
69347 | 17234 | glucose | + | growth |
69347 | 17268 | inositol | - | growth |
69347 | 37684 | mannose | + | growth |
69347 | 16634 | raffinose | - | growth |
69347 | 26546 | rhamnose | - | growth |
69347 | 17992 | sucrose | - | growth |
69347 | 18222 | xylose | + | growth |
68379 | 17632 | nitrate | - | reduction |
68379 | 4853 | esculin | + | hydrolysis |
68379 | 16199 | urea | - | hydrolysis |
68379 | 5291 | gelatin | + | hydrolysis |
68379 | 17634 | D-glucose | - | fermentation |
68379 | 16988 | D-ribose | - | fermentation |
68379 | 65327 | D-xylose | - | fermentation |
68379 | 16899 | D-mannitol | - | fermentation |
68379 | 17306 | maltose | - | fermentation |
68379 | 17716 | lactose | - | fermentation |
68379 | 17992 | sucrose | - | fermentation |
68379 | 28087 | glycogen | - | fermentation |
enzymes
@ref | value | activity | ec |
---|---|---|---|
68379 | gelatinase | + | |
68379 | urease | - | 3.5.1.5 |
68379 | beta-glucosidase | + | 3.2.1.21 |
68379 | N-acetyl-beta-glucosaminidase | + | 3.2.1.52 |
68379 | alpha-glucosidase | + | 3.2.1.20 |
68379 | beta-galactosidase | - | 3.2.1.23 |
68379 | beta-glucuronidase | - | 3.2.1.31 |
68379 | alkaline phosphatase | + | 3.1.3.1 |
68379 | pyrrolidonyl arylamidase | + | 3.4.19.3 |
68379 | pyrazinamidase | - | 3.5.1.B15 |
68382 | alkaline phosphatase | + | 3.1.3.1 |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | trypsin | + | 3.4.21.4 |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | alpha-glucosidase | + | 3.2.1.20 |
68382 | beta-glucosidase | + | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | + | 3.2.1.52 |
68382 | alpha-mannosidase | + | 3.2.1.24 |
API coryne
@ref | NIT | PYZ | PYRA | PAL | beta GUR | beta GAL | alpha GLU | beta NAG | ESC | URE | GEL | GLU | RIB | XYL | MAN | MAL | LAC | SAC | GLYG |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
69347 | - | - | + | + | - | - | + | + | + | - | + | - | - | - | - | - | - | - | - |
API zym
@ref | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
69347 | + | +/- | +/- | +/- | + | +/- | +/- | + | +/- | + | +/- | - | - | - | + | + | + | + | +/- |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | latitude | longitude |
---|---|---|---|---|---|---|---|
22662 | soil | Lushan Mountain, Jiangxi Province (29°42' N, 116°26' E) | China | CHN | Asia | 29.7 | 116.433 |
67770 | Soil in Lushan Mountain | China | CHN | Asia |
isolation source categories
- Cat1: #Environmental
- Cat2: #Terrestrial
- Cat3: #Soil
Safety information
risk assessment
- @ref: 22662
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 22662
- description: Streptomyces lushanensis strain JXJ 0135 16S ribosomal RNA gene, partial sequence
- accession: KF938656
- length: 1516
- database: ena
- NCBI tax ID: 1434255
Genome sequences
- @ref: 67770
- description: Streptomyces lushanensis NRRL B-24994
- accession: GCA_001700515
- assembly level: scaffold
- database: ncbi
- NCBI tax ID: 1434255
GC content
- @ref: 22662
- GC-content: 69.3
- method: high performance liquid chromatography (HPLC)
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | yes | 100 | no |
flagellated | no | 97.924 | no |
gram-positive | yes | 89.224 | no |
anaerobic | no | 99.032 | no |
halophile | no | 93.984 | no |
spore-forming | yes | 95.78 | no |
glucose-util | yes | 89.894 | no |
aerobic | yes | 93.314 | no |
thermophile | no | 97.274 | yes |
motile | no | 94.212 | no |
glucose-ferment | no | 91.478 | yes |
External links
@ref: 22662
culture collection no.: DSM 42121, JCM 19682, KACC 17834, KCTC 29261, JXJ 0135, JCM 19628, NRRL B-24994
straininfo link
- @ref: 89861
- straininfo: 396666
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 24984801 | Streptomyces lushanensis sp. nov., a novel actinomycete with anti-cyanobacterial activity. | Zhang BH, Cheng J, Chen W, Li HQ, Yang JY, Park DJ, Kim CJ, Shen R, Duan YQ, Li WJ | J Antibiot (Tokyo) | 10.1038/ja.2014.85 | 2014 | Anti-Bacterial Agents/isolation & purification/*pharmacology, Base Composition, China, Cyanobacteria/*drug effects, DNA, Bacterial/*genetics, Fatty Acids/chemistry/isolation & purification, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/chemistry/isolation & purification, Phylogeny, RNA, Ribosomal, 16S/genetics, *Soil Microbiology, Spores, Bacterial/chemistry, Streptomyces/*classification/genetics/isolation & purification | Enzymology |
Phylogeny | 30993571 | Streptomyces corynorhini sp. nov., isolated from Townsend's big-eared bats (Corynorhinus townsendii). | Hamm PS, Caimi NA, Northup DE, Valdez EW, Buecher DC, Dunlap CA, Labeda DP, Porras-Alfaro A | Antonie Van Leeuwenhoek | 10.1007/s10482-019-01261-z | 2019 | Animals, Bacterial Typing Techniques, Base Composition, Chiroptera/*microbiology, Metabolic Networks and Pathways/genetics, Microscopy, Electron, Scanning, Multilocus Sequence Typing, New Mexico, Nucleic Acid Hybridization, Phylogeny, Spores, Bacterial/ultrastructure, Streptomyces/*classification/genetics/*isolation & purification/physiology, Whole Genome Sequencing | Enzymology |
Phylogeny | 31674894 | Streptomyces paludis sp. nov., isolated from an alpine wetland soil. | Zhao J, Tang X, Li K, Guo Y, Feng M, Gao J | Int J Syst Evol Microbiol | 10.1099/ijsem.0.003821 | 2020 | Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Multilocus Sequence Typing, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Streptomyces/*classification/isolation & purification, Vitamin K 2/analogs & derivatives/chemistry, *Wetlands | Transcriptome |
Reference
@id | authors | title | doi/url | catalogue |
---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
22662 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-42121 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 42121) | |
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||
68379 | Automatically annotated from API Coryne | |||
68382 | Automatically annotated from API zym | |||
69347 | Wink, J. | Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig | https://cdn.dsmz.de/wink/DSM%2042121.pdf | |
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | Predictions based on the model GenomeNet Sporulation v. 1 | 10.21203/rs.3.rs-2527258/v1 | |
89861 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID396666.1 |