General

@ref: 22637

BacDive-ID: 131410

DSM-Number: 23598

keywords: 16S sequence, Bacteria, anaerobe, mesophilic

description: Clostridium amazonense NE08V is an anaerobe, mesophilic bacterium that was isolated from human fecal sample of an individual residing in a remote Amazonian community.

NCBI tax id

• NCBI tax id: 1648214
• Matching level: species

strain history

• @ref: 22637
• history: <- P. Lawson, Dept. Botany and Microbiol., Univ. Oklahoma, USA; NE08V <- L. O'Neal {2010}

doi: 10.13145/bacdive131410.20230509.8.1

Name and taxonomic classification

LPSN

• @ref: 20215
• description: domain/bacteria
• keyword: phylum/bacillota
• domain: Bacteria
• phylum: Bacillota
• class: Clostridia
• order: Eubacteriales
• family: Clostridiaceae
• genus: Clostridium
• species: Clostridium amazonense
• full scientific name: Clostridium amazonense O'Neal et al. 2015

@ref: 22637

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Clostridiaceae

genus: Clostridium

species: Clostridium amazonense

full scientific name: Clostridium amazonense O'Neal et al. 2015

strain designation: NE08V

type strain: yes

Culture and growth conditions

culture medium

• @ref: 22637
• name: PY + X MEDIUM (DSMZ Medium 104b)
• growth: yes
• link: https://mediadive.dsmz.de/medium/104b
• composition: Name: PY + X MEDIUM (DSMZ Medium 104b) Composition: Yeast extract 10.0 g/l D-Glucose 5.0 g/l Trypticase peptone 5.0 g/l Meat peptone 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l KH2PO4 0.04 g/l K2HPO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Sodium resazurin 0.0005 g/l Distilled water

culture temp

Physiology and metabolism

oxygen tolerance

Isolation, sampling and environmental information

isolation

isolation source categories

taxonmaps

• @ref: 69479
• File name: preview.99_1205.png
• url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_34;96_705;97_823;98_954;99_1205&stattab=map
• Last taxonomy: Clostridium
• 16S sequence: KP281434
• Sequence Identity:
• Total samples: 8762
• soil counts: 795
• aquatic counts: 3408
• animal counts: 4411
• plant counts: 148

Safety information

risk assessment

• @ref: 22637
• biosafety level: 1
• biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

• @ref: 22637
• description: Clostridium amazonense strain NE08V 16S ribosomal RNA gene, partial sequence
• accession: KP281434
• length: 1430
• database: ena
• NCBI tax ID: 1648214

GC content

• @ref: 22637
• GC-content: 31.6
• method: high performance liquid chromatography (HPLC)

External links

@ref: 22637

culture collection no.: DSM 23598, CCUG 59712

straininfo link

• @ref: 89840
• straininfo: 404182

literature

• topic: Phylogeny
• Pubmed-ID: 26123611
• title: Clostridium amazonense sp. nov. an obliqately anaerobic bacterium isolated from a remote Amazonian community in Peru.
• authors: O'Neal L, Obregon-Tito AJ, Tito RY, Ozga AT, Polo SI, Lewis CM Jr, Lawson PA
• journal: Anaerobe
• DOI: 10.1016/j.anaerobe.2015.06.005
• year: 2015
• mesh: Bacterial Typing Techniques, Base Composition, Clostridium/*classification/*isolation & purification, Cluster Analysis, Cytosol/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Feces/*microbiology, Humans, Molecular Sequence Data, Peru, Phylogeny, Population Groups, RNA, Ribosomal, 16S/genetics, Rural Population, Sequence Analysis, DNA
• topic2: Pathogenicity

Reference