Strain identifier
BacDive ID: 131362
Type strain: 
Species: Pullulanibacillus pueri
Strain Designation: YN3
Strain history: L. Niu YN3.
NCBI tax ID(s): 1437324 (species)
version 8.1 (current version)
General
@ref: 22589
BacDive-ID: 131362
DSM-Number: 100927
keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic, motile
description: Pullulanibacillus pueri YN3 is a spore-forming, mesophilic, motile bacterium that was isolated from ripened Pu'er tea.
NCBI tax id
- NCBI tax id: 1437324
- Matching level: species
strain history
| @ref | history |
|---|---|
| 22589 | <- JCM <- L. Niu, Shanghai Key Laboratory of Bio-Energy Crops, Shanghai University; YN3 |
| 67770 | L. Niu YN3. |
doi: 10.13145/bacdive131362.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Caryophanales
- family: Sporolactobacillaceae
- genus: Pullulanibacillus
- species: Pullulanibacillus pueri
- full scientific name: Pullulanibacillus pueri Niu et al. 2015
@ref: 22589
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Bacillales
family: Sporolactobacillaceae
genus: Pullulanibacillus
species: Pullulanibacillus pueri
full scientific name: Pullulanibacillus pueri Niu et al. 2015
strain designation: YN3
type strain: yes
Morphology
cell morphology
| @ref | motility | confidence | gram stain |
|---|---|---|---|
| 69480 | yes | 94.705 | |
| 69480 | 100 | positive |
Culture and growth conditions
culture medium
- @ref: 22589
- name: ALICYCLOBACILLUS MEDIUM (DSMZ Medium 402)
- growth: yes
- link: https://mediadive.dsmz.de/medium/402
- composition: Name: ALICYCLOBACILLUS MEDIUM (DSMZ Medium 402) Composition: Agar 15.0 g/l Glucose 5.0 g/l KH2PO4 3.0 g/l Yeast extract 2.0 g/l MgSO4 x 7 H2O 0.5 g/l CaCl2 x 2 H2O 0.25 g/l (NH4)2SO4 0.2 g/l H3BO3 0.0003 g/l CoCl2 x 6 H2O 0.0002 g/l ZnSO4 x 7 H2O 0.0001 g/l MnCl2 x 4 H2O 3e-05 g/l Na2MoO4 x 2 H2O 3e-05 g/l NiCl2 x 6 H2O 2e-05 g/l CuCl2 x 2 H2O 1e-05 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 22589 | positive | growth | 37 | mesophilic |
| 67770 | positive | growth | 28 | mesophilic |
Physiology and metabolism
spore formation
- @ref: 69480
- spore formation: yes
- confidence: 100
observation
- @ref: 67770
- observation: quinones: MK-7
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent |
|---|---|---|---|---|---|
| 22589 | ripened Pu'er tea | Puer City, Yunnan Province | China | CHN | Asia |
| 67770 | Ripened Pu'er tea | China | CHN | Asia |
isolation source categories
- Cat1: #Engineered
- Cat2: #Food production
- Cat3: #Beverage
taxonmaps
- @ref: 69479
- File name: preview.99_45385.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_414;96_8908;97_25908;98_32902;99_45385&stattab=map
- Last taxonomy: Pullulanibacillus pueri
- 16S sequence: KF733796
- Sequence Identity:
- Total samples: 59
- soil counts: 43
- aquatic counts: 5
- animal counts: 4
- plant counts: 7
Safety information
risk assessment
- @ref: 22589
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 22589
- description: Pullulanibacillus pueri strain YN3 16S ribosomal RNA gene, partial sequence
- accession: KF733796
- length: 1553
- database: ena
- NCBI tax ID: 1437324
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Pullulanibacillus pueri CGMCC 1.12777 | GCA_014639255 | contig | ncbi | 1437324 |
| 66792 | Pullulanibacillus pueri DSM 100927 | GCA_016908895 | contig | ncbi | 1437324 |
| 66792 | Pullulanibacillus pueri strain CGMCC 1.12777 | 1437324.3 | wgs | patric | 1437324 |
| 66792 | Pullulanibacillus pueri strain DSM 100927 | 1437324.4 | wgs | patric | 1437324 |
| 66792 | Pullulanibacillus pueri DSM 100927 | 2901228672 | draft | img | 1437324 |
GC content
| @ref | GC-content | method |
|---|---|---|
| 22589 | 38.7 | |
| 67770 | 38.7 | thermal denaturation, midpoint method (Tm) |
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | yes | 87 | no |
| motile | yes | 86.492 | no |
| gram-positive | yes | 78.892 | no |
| anaerobic | no | 98.649 | no |
| halophile | yes | 50.864 | no |
| spore-forming | yes | 96.226 | no |
| thermophile | no | 94.053 | no |
| glucose-util | yes | 89.198 | no |
| aerobic | yes | 87.547 | no |
| flagellated | yes | 70.579 | no |
| glucose-ferment | no | 83.616 | no |
External links
@ref: 22589
culture collection no.: DSM 100927, CGMCC 1.12777, JCM 30075
straininfo link
- @ref: 89801
- straininfo: 399667
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 25858244 | Pullulanibacillus pueri sp. nov., isolated from Pu'er tea. | Niu L, Tang T, Song L, Xiong M, Tian J, Zhang K, Hu X, Zhu D | Int J Syst Evol Microbiol | 10.1099/ijs.0.000239 | 2015 | Bacillales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Tea/*microbiology, Vitamin K 2/analogs & derivatives/chemistry | Genetics |
| Phylogeny | 29789976 | Scopulibacillus cellulosilyticus sp. nov., a cellulose-degrading bacterium isolated from tea. | Yan ZF, Lin P, Li CT, Kook M, Yi TH | Antonie Van Leeuwenhoek | 10.1007/s10482-018-1101-1 | 2018 | Bacteria/classification/genetics/*metabolism, Cellulose/*metabolism, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Tea/*microbiology | Metabolism |
Reference
| @id | authors | title | doi/url | catalogue |
|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
| 22589 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-100927 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 100927) | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | |
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
| 89801 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID399667.1 |