Strain identifier

BacDive ID: 131360

Type strain: Yes

Species: Lactococcus laudensis

Strain Designation: 4195, CRA-FLC-4195

Strain history: CIP <- 2015, M. Zago, CRA-FLC, Lodi, Italy: strain 4195

NCBI tax ID(s): 1494461 (species)

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General

@ref: 22587

BacDive-ID: 131360

DSM-Number: 28961

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic

description: Lactococcus laudensis 4195 is a microaerophile, mesophilic bacterium that was isolated from cow raw milk.

NCBI tax id

  • NCBI tax id: 1494461
  • Matching level: species

strain history

@refhistory
22587<- M. Zago, CRA-FLC, Lodi, Italy; CRA-FLC-4195
67770LMG 28353 <-- M. Zago; CRA-FLC, Italy; 4195.
120571CIP <- 2015, M. Zago, CRA-FLC, Lodi, Italy: strain 4195

doi: 10.13145/bacdive131360.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Streptococcaceae
  • genus: Lactococcus
  • species: Lactococcus laudensis
  • full scientific name: Lactococcus laudensis Meucci et al. 2015

@ref: 22587

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Streptococcaceae

genus: Lactococcus

species: Lactococcus laudensis

full scientific name: Lactococcus laudensis Meucci et al. 2015

strain designation: 4195, CRA-FLC-4195

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no92.789
69480100positive
120571nopositivecoccus-shaped

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
22587MRS MEDIUM (DSMZ Medium 11)yeshttps://mediadive.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
120571CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40
120571CIP Medium 29yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=29

culture temp

@refgrowthtypetemperaturerange
22587positivegrowth30mesophilic
67770positivegrowth30mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 22587
  • oxygen tolerance: microaerophile

spore formation

@refspore formationconfidence
69481no100
69480no99.998

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen+builds acid from28087
68371starch+builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose+builds acid from17992
68371melibiose-builds acid from28053
68371lactose+builds acid from17716
68371maltose+builds acid from17306
68371cellobiose+builds acid from17057
68371salicin+builds acid from17814
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose+builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
22587-----++----+++--------+--+/-++++-+----++------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
22587cow raw milkLombardy, Valle TrompiaItalyITAEurope
67770Cow milk collected from a bovine herd in Valle TrompiaItalyITAEurope
120571Food, Cow, raw milkLombardy, ItalyItalyITAEurope1998-03-02

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Bovinae (Cow, Cattle)
#Host Body Product#Fluids#Milk

taxonmaps

  • @ref: 69479
  • File name: preview.99_6527.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_58;96_1605;97_3793;98_4816;99_6527&stattab=map
  • Last taxonomy: Lactococcus
  • 16S sequence: KJ394457
  • Sequence Identity:
  • Total samples: 6191
  • soil counts: 815
  • aquatic counts: 1196
  • animal counts: 4033
  • plant counts: 147

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
225871Risk group (German classification)
1205711Risk group (French classification)

Sequence information

16S sequences

  • @ref: 22587
  • description: Lactococcus laudensis strain DSM 28961 16S ribosomal RNA gene, partial sequence
  • accession: KJ394457
  • length: 1478
  • database: ena
  • NCBI tax ID: 1494461

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Lactococcus laudensis LMG 28353GCA_019586025contigncbi1494461
66792Lactococcus laudensis strain DSM 289611494461.5wgspatric1494461
66792Lactococcus laudensis strain LMG 283531494461.9wgspatric1494461
67770Lactococcus laudensis DSM 28961GCA_013449735contigncbi1494461

GC content

@refGC-contentmethod
2258741.1fluorimetric
6777041.1thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno96.25no
gram-positiveyes96.889no
anaerobicno91.1yes
aerobicno96.985yes
halophileyes76.411no
spore-formingno94.909no
thermophileno99.768no
glucose-utilyes86.373no
flagellatedno97.964no
glucose-fermentyes90.773no

External links

@ref: 22587

culture collection no.: DSM 28961, LMG 28353, JCM 31965, CIP 110947

straininfo link

  • @ref: 89799
  • straininfo: 394243

literature

  • topic: Phylogeny
  • Pubmed-ID: 25833154
  • title: Lactococcus hircilactis sp. nov. and Lactococcus laudensis sp. nov., isolated from milk.
  • authors: Meucci A, Zago M, Rossetti L, Fornasari ME, Bonvini B, Tidona F, Povolo M, Contarini G, Carminati D, Giraffa G
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.000225
  • year: 2015
  • mesh: Animals, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Genes, Bacterial, *Goats, Italy, Lactococcus/*classification/genetics/isolation & purification, Milk/*microbiology, Molecular Sequence Data, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
  • topic2: Genetics

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
22587Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-28961Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 28961)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
89799Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID394243.1
120571Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20110947Collection of Institut Pasteur (CIP 110947)