Strain identifier
BacDive ID: 131252
Type strain:
Species: Cupriavidus alkaliphilus
Strain Designation: ASC-732
Strain history: CIP <- 2011, P. Estrada de los Santos, UNAM, Cuernavaca, Morelos, Mexico: strain ASC-732
NCBI tax ID(s): 942866 (species)
General
@ref: 22479
BacDive-ID: 131252
DSM-Number: 28880
keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-negative, oval-shaped
description: Cupriavidus alkaliphilus ASC-732 is an obligate aerobe, mesophilic, Gram-negative bacterium that was isolated from agave rhizosphere.
NCBI tax id
- NCBI tax id: 942866
- Matching level: species
strain history
@ref | history |
---|---|
22479 | <- CIP <- P. Estrada-de los Santos, Centro de Ciencias Genómicas Universidad Nacional Autonoma de Mexico; ASC-732 |
116245 | CIP <- 2011, P. Estrada de los Santos, UNAM, Cuernavaca, Morelos, Mexico: strain ASC-732 |
doi: 10.13145/bacdive131252.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Betaproteobacteria
- order: Burkholderiales
- family: Burkholderiaceae
- genus: Cupriavidus
- species: Cupriavidus alkaliphilus
- full scientific name: Cupriavidus alkaliphilus Estrada-de Los Santos et al. 2012
@ref: 22479
domain: Bacteria
phylum: Proteobacteria
class: Betaproteobacteria
order: Burkholderiales
family: Burkholderiaceae
genus: Cupriavidus
species: Cupriavidus alkaliphilus
full scientific name: Cupriavidus alkaliphilus Estrada-de Los Santos et al. 2012
strain designation: ASC-732
type strain: yes
Morphology
cell morphology
- @ref: 116245
- gram stain: negative
- cell shape: oval-shaped
- motility: no
multimedia
- @ref: 22479
- multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_28880.jpg
- intellectual property rights: © Leibniz-Institut DSMZ
Culture and growth conditions
culture medium
@ref | name | growth | composition | link |
---|---|---|---|---|
39790 | MEDIUM 72- for trypto casein soja agar | yes | Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g) | |
22479 | TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) | yes | Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water | https://mediadive.dsmz.de/medium/535 |
116245 | CIP Medium 72 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72 | |
116245 | CIP Medium 368 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=368 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
22479 | positive | growth | 28 | mesophilic |
39790 | positive | growth | 30 | mesophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 116245
- oxygen tolerance: obligate aerobe
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
68369 | 25115 | malate | + | assimilation |
68369 | 17128 | adipate | - | assimilation |
68369 | 27689 | decanoate | + | assimilation |
68369 | 24265 | gluconate | + | assimilation |
68369 | 17306 | maltose | - | assimilation |
68369 | 59640 | N-acetylglucosamine | - | assimilation |
68369 | 16899 | D-mannitol | - | assimilation |
68369 | 16024 | D-mannose | - | assimilation |
68369 | 30849 | L-arabinose | - | assimilation |
68369 | 17634 | D-glucose | - | assimilation |
68369 | 5291 | gelatin | - | hydrolysis |
68369 | 4853 | esculin | - | hydrolysis |
68369 | 16199 | urea | - | hydrolysis |
68369 | 29016 | arginine | - | hydrolysis |
68369 | 17634 | D-glucose | - | fermentation |
68369 | 27897 | tryptophan | - | energy source |
68369 | 17632 | nitrate | + | reduction |
68368 | 30849 | L-arabinose | - | fermentation |
68368 | 27613 | amygdalin | - | fermentation |
68368 | 28053 | melibiose | - | fermentation |
68368 | 17992 | sucrose | - | fermentation |
68368 | 62345 | L-rhamnose | - | fermentation |
68368 | 30911 | sorbitol | - | fermentation |
68368 | 17268 | myo-inositol | - | fermentation |
68368 | 16899 | D-mannitol | - | fermentation |
68368 | 17634 | D-glucose | - | fermentation |
68368 | 5291 | gelatin | - | hydrolysis |
68368 | 27897 | tryptophan | - | energy source |
68368 | 16199 | urea | - | hydrolysis |
68368 | 16947 | citrate | - | assimilation |
68368 | 18257 | ornithine | - | degradation |
68368 | 25094 | lysine | - | degradation |
68368 | 29016 | arginine | - | hydrolysis |
116245 | 17632 | nitrate | + | reduction |
116245 | 16301 | nitrite | + | reduction |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
68369 | 35581 | indole | no |
68368 | 15688 | acetoin | no |
68368 | 35581 | indole | no |
68368 | 16136 | hydrogen sulfide | no |
116245 | 35581 | indole | no |
metabolite tests
@ref | Chebi-ID | metabolite | indole test | voges-proskauer-test |
---|---|---|---|---|
68369 | 35581 | indole | - | |
68368 | 15688 | acetoin | - | |
68368 | 35581 | indole | - |
enzymes
@ref | value | activity | ec |
---|---|---|---|
68369 | cytochrome oxidase | + | 1.9.3.1 |
68369 | gelatinase | - | |
68369 | beta-glucosidase | - | 3.2.1.21 |
68369 | urease | - | 3.5.1.5 |
68369 | arginine dihydrolase | - | 3.5.3.6 |
68368 | cytochrome oxidase | + | 1.9.3.1 |
68368 | gelatinase | - | |
68368 | tryptophan deaminase | - | 4.1.99.1 |
68368 | urease | - | 3.5.1.5 |
68368 | ornithine decarboxylase | - | 4.1.1.17 |
68368 | lysine decarboxylase | - | 4.1.1.18 |
68368 | arginine dihydrolase | - | 3.5.3.6 |
68368 | beta-galactosidase | - | 3.2.1.23 |
116245 | oxidase | + | |
116245 | catalase | + | 1.11.1.6 |
116245 | urease | - | 3.5.1.5 |
68382 | alkaline phosphatase | + | 3.1.3.1 |
68382 | esterase (C 4) | + | |
68382 | esterase lipase (C 8) | - | |
68382 | lipase (C 14) | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | valine arylamidase | - | |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | trypsin | - | 3.4.21.4 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | naphthol-AS-BI-phosphohydrolase | - | |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
116245 | - | + | + | - | - | + | - | - | - | - | + | - | - | - | - | - | - | - | - | - |
API 20E
@ref | ONPG | ADH Arg | LDC Lys | ODC | CIT | H2S | URE | TDA Trp | IND | VP | GEL | GLU | MAN | INO | Sor | RHA | SAC | MEL | AMY | ARA | OX |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
22479 | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | + |
API 20NE
@ref | NO3 | TRP | GLU_ Ferm | ADH Arg | URE | ESC | GEL | PNPG | GLU_ Assim | ARA | MNE | MAN | NAG | MAL | GNT | CAP | ADI | MLT | CIT | PAC | OX |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
22479 | + | - | - | - | - | - | - | - | - | - | - | - | - | - | + | + | - | + | - | + | + |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | isolation date |
---|---|---|---|---|---|---|
22479 | agave rhizosphere | Tamaulipas, San Carlos | Mexico | MEX | North America | |
116245 | Environment, Agave rhizosphere | San Carlos, Tamaulipas | Mexico | MEX | North America | 2008 |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Host | #Plants | #Herbaceous plants (Grass,Crops) |
#Host Body-Site | #Plant | #Rhizosphere |
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
22479 | 1 | Risk group (German classification) |
116245 | 1 | Risk group (French classification) |
Sequence information
16S sequences
- @ref: 22479
- description: Cupriavidus alkaliphilus strain ASC-732 16S ribosomal RNA gene, partial sequence
- accession: HQ438078
- length: 1520
- database: ena
- NCBI tax ID: 942866
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Cupriavidus alkaliphilus ASC-732 | GCA_900094595 | scaffold | ncbi | 942866 |
66792 | Cupriavidus alkaliphilus ASC-732 | 2671180232 | draft | img | 942866 |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
motile | yes | 87.54 | no |
flagellated | no | 62.259 | no |
gram-positive | no | 98.139 | no |
anaerobic | no | 99.046 | no |
aerobic | yes | 94.537 | no |
halophile | no | 94.011 | no |
spore-forming | no | 93.853 | no |
glucose-ferment | no | 90.644 | yes |
thermophile | no | 98.255 | yes |
glucose-util | no | 69.17 | yes |
External links
@ref: 22479
culture collection no.: DSM 28880, CIP 110330, LMG 26294
straininfo link
- @ref: 89696
- straininfo: 375958
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 22738764 | Cupriavidus alkaliphilus sp. nov., a new species associated with agricultural plants that grow in alkaline soils. | Estrada-de los Santos P, Martinez-Aguilar L, Lopez-Lara IM, Caballero-Mellado J | Syst Appl Microbiol | 10.1016/j.syapm.2012.05.005 | 2012 | Agriculture, Base Composition, Cupriavidus/*classification/genetics/*isolation & purification, Hydrogen-Ion Concentration, Mexico, Phylogeny, *Rhizosphere, Soil/chemistry, Soil Microbiology | Enzymology |
Phylogeny | 27001671 | Cupriavidus nantongensis sp. nov., a novel chlorpyrifos-degrading bacterium isolated from sludge. | Sun LN, Wang DS, Yang ED, Fang LC, Chen YF, Tang XY, Hua RM | Int J Syst Evol Microbiol | 10.1099/ijsem.0.001034 | 2016 | Bacterial Typing Techniques, Base Composition, China, Chlorpyrifos/*metabolism, Cupriavidus/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sewage/*microbiology, Ubiquinone/chemistry | Transcriptome |
Genetics | 27660789 | Draft Genome Sequence of Heavy Metal-Resistant Cupriavidus alkaliphilus ASC-732T, Isolated from Agave Rhizosphere in the Northeast of Mexico. | Rojas-Rojas FU, Huntemann M, Clum A, Pillay M, Palaniappan K, Varghese N, Mikhailova N, Stamatis D, Reddy TB, Markowitz V, Ivanova N, Kyrpides N, Woyke T, Shapiro N, Ibarra JA, Estrada-de Los Santos P | Genome Announc | 10.1128/genomeA.01013-16 | 2016 | Phylogeny | |
Phylogeny | 30317454 | Cupriavidus lacunae sp. nov., isolated from pond-side soil. | Feng T, Kim KH, Oh J, Jeon CO | Antonie Van Leeuwenhoek | 10.1007/s10482-018-1187-5 | 2018 | Bacterial Typing Techniques, Base Composition, Cupriavidus/classification/genetics/*isolation & purification/metabolism, DNA, Bacterial/genetics, Fatty Acids/chemistry/metabolism, Ponds/*microbiology, RNA, Ribosomal, 16S/genetics, Republic of Korea, Soil/chemistry, *Soil Microbiology | Metabolism |
Metabolism | 31022352 | Comparative genome analysis of completely sequenced Cupriavidus genomes provides insights into the biosynthetic potential and versatile applications of Cupriavidus alkaliphilus ASC-732. | Kutralam-Muniasamy G, Perez-Guevara F | Can J Microbiol | 10.1139/cjm-2019-0027 | 2019 | Bacterial Proteins/genetics/metabolism, Cupriavidus/enzymology/*genetics/metabolism, *Genome, Bacterial, Mexico, Pentose Phosphate Pathway, Photosynthesis, Polyhydroxyalkanoates/biosynthesis, Polysaccharides, Bacterial/biosynthesis, Rhizosphere | Genetics |
Reference
@id | authors | title | doi/url | catalogue |
---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
22479 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-28880 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 28880) | |
39790 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/8177 | ||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
68368 | Automatically annotated from API 20E | |||
68369 | Automatically annotated from API 20NE | |||
68382 | Automatically annotated from API zym | |||
89696 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID375958.1 | |
116245 | Curators of the CIP | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20110330 | Collection of Institut Pasteur (CIP 110330) |