Strain identifier

BacDive ID: 131178

Type strain: Yes

Species: Anaerobacillus alkalilacustris

Strain Designation: Z-0521

Strain history: <- D. G. Zavarzina, Winogradsky Inst. Microbiol., RAS, Moscow, Russia; Z-0521 <- D. G. Zavarzina {2005}

NCBI tax ID(s): 393763 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 22470

BacDive-ID: 131178

DSM-Number: 18345

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, mesophilic, motile

description: Anaerobacillus alkalilacustris Z-0521 is an anaerobe, spore-forming, mesophilic bacterium that was isolated from deposits of a low-salt soda lake.

NCBI tax id

  • NCBI tax id: 393763
  • Matching level: species

strain history

  • @ref: 22470
  • history: <- D. G. Zavarzina, Winogradsky Inst. Microbiol., RAS, Moscow, Russia; Z-0521 <- D. G. Zavarzina {2005}

doi: 10.13145/bacdive131178.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Anaerobacillus
  • species: Anaerobacillus alkalilacustris
  • full scientific name: Anaerobacillus alkalilacustris corrig. Zavarzina et al. 2010
  • synonyms

    • @ref: 20215
    • synonym: Anaerobacillus alkalilacustre

@ref: 22470

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Anaerobacillus

species: Anaerobacillus alkalilacustris

full scientific name: Anaerobacillus alkalilacustris Zavarzina et al. 2010

strain designation: Z-0521

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram stain
69480yes98.036
69480100positive

Culture and growth conditions

culture medium

  • @ref: 22470
  • name: ANAEROBACILLUS MEDIUM (DSMZ Medium 898a)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/898a
  • composition: Name: ANAEROBACILLUS MEDIUM (DSMZ Medium 898a; with strain-specific modifications) Composition: NaCl 14.9701 g/l NaHCO3 9.98004 g/l Na2CO3 2.75449 g/l D-Mannitol 2.49501 g/l NH4Cl 0.998004 g/l Na2S x 9 H2O 0.499002 g/l Yeast extract 0.499002 g/l KH2PO4 0.199601 g/l KCl 0.199601 g/l MgCl2 x 6 H2O 0.0998004 g/l HCl 0.00249501 g/l FeCl2 x 4 H2O 0.00149701 g/l Sodium resazurin 0.000499002 g/l CoCl2 x 6 H2O 0.000189621 g/l MnCl2 x 4 H2O 9.98004e-05 g/l Pyridoxine hydrochloride 9.98004e-05 g/l ZnCl2 6.98603e-05 g/l (DL)-alpha-Lipoic acid 4.99002e-05 g/l p-Aminobenzoic acid 4.99002e-05 g/l Thiamine HCl 4.99002e-05 g/l Calcium D-(+)-pantothenate 4.99002e-05 g/l Riboflavin 4.99002e-05 g/l Nicotinic acid 4.99002e-05 g/l Na2MoO4 x 2 H2O 3.59281e-05 g/l NiCl2 x 6 H2O 2.39521e-05 g/l Folic acid 1.99601e-05 g/l Biotin 1.99601e-05 g/l H3BO3 5.98802e-06 g/l CuCl2 x 2 H2O 1.99601e-06 g/l Vitamin B12 9.98004e-07 g/l Distilled water

culture temp

  • @ref: 22470
  • growth: positive
  • type: growth
  • temperature: 35
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 22470
  • oxygen tolerance: anaerobe

spore formation

@refspore formationconfidence
69481yes100
69480yes100

Isolation, sampling and environmental information

isolation

  • @ref: 22470
  • sample type: deposits of a low-salt soda lake
  • geographic location: Siberia, Tuva Republic, lake Khadyn
  • country: Russia
  • origin.country: RUS
  • continent: Asia

isolation source categories

Cat1Cat2Cat3
#Engineered#Waste#Solid waste
#Environmental#Aquatic#Lake (large)
#Condition#Alkaline

taxonmaps

  • @ref: 69479
  • File name: preview.99_7342.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_1885;97_2264;98_5385;99_7342&stattab=map
  • Last taxonomy: Anaerobacillus
  • 16S sequence: DQ675454
  • Sequence Identity:
  • Total samples: 1562
  • soil counts: 346
  • aquatic counts: 237
  • animal counts: 952
  • plant counts: 27

Safety information

risk assessment

  • @ref: 22470
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 22470
  • description: Anaerobacillus alkalilacustris strain Z-0521 16S ribosomal RNA gene, partial sequence
  • accession: DQ675454
  • length: 1516
  • database: ena
  • NCBI tax ID: 393763

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Anaerobacillus alkalilacustris DSM 18345GCA_001866005contigncbi393763
66792Anaerobacillus alkalilacustris strain DSM 18345393763.5wgspatric393763
66792Anaerobacillus alkalilacustris DSM 183452698536992draftimg393763

GC content

  • @ref: 22470
  • GC-content: 36.2
  • method: thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
gram-positiveyes86.046no
anaerobicno74.76no
halophileyes55.499no
spore-formingyes92.629no
glucose-utilyes85.84no
aerobicno87.722yes
motileyes83.761no
flagellatedyes69.471no
thermophileno91.341yes
glucose-fermentno79.815no

External links

@ref: 22470

culture collection no.: DSM 18345, DSMZ 18345, VKM B-2403

straininfo link

  • @ref: 89623
  • straininfo: 397382

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
22470Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-18345Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 18345)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar SchomburgBRENDA, the ELIXIR core data resource in 2021: new developments and updates10.1093/nar/gkaa1025Nucleic Acids Res. 49: D498-D508 202033211880
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
89623Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID397382.1