Strain identifier

BacDive ID: 131104

Type strain: Yes

Species: Enterobacter hormaechei subsp. steigerwaltii

Strain Designation: EN-562

Strain history: CIP <- 2004, J. Heesemann, LM Munich Univ., Gauting, Germany: strain EN-562 <- D. Pierard

NCBI tax ID(s): 299766 (subspecies)

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General

@ref: 22396

BacDive-ID: 131104

DSM-Number: 16691

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Enterobacter hormaechei subsp. steigerwaltii EN-562 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from infected surgical skin wound of a 49-year-old patient with tonsillar carcinoma.

NCBI tax id

  • NCBI tax id: 299766
  • Matching level: subspecies

strain history

@refhistory
22396<- H. Hoffmann, Institute of Microbiology, Pneumological Teaching Hospital of the University of Munich; EN-562 <- D. Pierard, Academisch Ziekenhuis Vrije Universiteit Brussels
119968CIP <- 2004, J. Heesemann, LM Munich Univ., Gauting, Germany: strain EN-562 <- D. Pierard

doi: 10.13145/bacdive131104.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Enterobacteriaceae
  • genus: Enterobacter
  • species: Enterobacter hormaechei subsp. steigerwaltii
  • full scientific name: Enterobacter hormaechei subsp. steigerwaltii Hoffmann et al. 2016

@ref: 22396

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacteriales, not validated

family: Enterobacteriaceae

genus: Enterobacter

species: Enterobacter hormaechei subsp. steigerwaltii

full scientific name: Enterobacter hormaechei subsp. steigerwaltii Hoffmann et al. 2016

strain designation: EN-562

type strain: yes

Morphology

cell morphology

  • @ref: 119968
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
22396TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
22396CASO AGAR (MERCK 105458) (DSMZ Medium 220)yeshttps://mediadive.dsmz.de/medium/220Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
41957MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
22396COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
119968CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
22396positivegrowth37mesophilic
41957positivegrowth37mesophilic
60072positivegrowth30-37mesophilic
119968positivegrowth10-41
119968nogrowth5psychrophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
60072aerobe
119968facultative anaerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836830849L-arabinose+fermentation
6836827613amygdalin+fermentation
6836828053melibiose+fermentation
6836817992sucrose+fermentation
6836862345L-rhamnose+fermentation
6836830911sorbitol+fermentation
6836817268myo-inositol+fermentation
6836816899D-mannitol+fermentation
6836817634D-glucose+fermentation
683685291gelatin-hydrolysis
6836827897tryptophan-energy source
6836816199urea-hydrolysis
6836816947citrate+assimilation
6836818257ornithine+degradation
6836825094lysine-degradation
6836829016arginine+hydrolysis
11996829864mannitol+fermentation
11996816947citrate+carbon source
11996817234glucose+fermentation
11996817716lactose-fermentation
11996817632nitrate+reduction
11996816301nitrite-reduction
11996815792malonate+assimilation
119968132112sodium thiosulfate-builds gas from

antibiotic resistance

  • @ref: 119968
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinno
6836835581indoleno
6836816136hydrogen sulfideno
11996835581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole testmethylred-test
6836815688acetoin-
6836835581indole-
11996815688acetoin+
11996817234glucose-

enzymes

@refvalueactivityec
68368cytochrome oxidase-1.9.3.1
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase+4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase+3.2.1.23
119968oxidase-
119968beta-galactosidase+3.2.1.23
119968alcohol dehydrogenase+1.1.1.1
119968gelatinase-
119968catalase+1.11.1.6
119968lysine decarboxylase-4.1.1.18
119968ornithine decarboxylase+4.1.1.17
119968phenylalanine ammonia-lyase-4.3.1.24
119968tryptophan deaminase-
119968urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119968-+++-++-+-++++-+-+--

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOX
22396++-++------+++++++++-

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
119968+++++-++++++-++++++++++++-+-+--++++-++-+-++++---++++-++++--+++-------------+---++-+---+--++++++--+-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudesampling dateisolation date
22396infected surgical skin wound of a 49-year-old patient with tonsillar carcinomaBrusselsBelgiumBELEurope50.85064.3489
60072Human surgical woundBrusselsBelgiumBELEurope2002
119968Surgical wound infectionBrusselsBelgiumBELEurope2002

isolation source categories

Cat1Cat2Cat3
#Infection#Disease
#Infection#Patient
#Host Body-Site#Other#Wound
#Host Body-Site#Organ#Skin, Nail, Hair

taxonmaps

  • @ref: 69479
  • File name: preview.99_120.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_6;96_7;97_7;98_17;99_120&stattab=map
  • Last taxonomy: Enterobacteriaceae
  • 16S sequence: AJ853890
  • Sequence Identity:
  • Total samples: 13334
  • soil counts: 615
  • aquatic counts: 1353
  • animal counts: 10132
  • plant counts: 1234

Safety information

risk assessment

  • @ref: 119968
  • biosafety level: 2
  • biosafety level comment: Risk group (French classification)

Sequence information

16S sequences

  • @ref: 22396
  • description: Enterobacter hormaechei subsp. steigerwaltii 16S rRNA gene, type strain EN-562T
  • accession: AJ853890
  • length: 1514
  • database: ena
  • NCBI tax ID: 299766

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Enterobacter hormaechei subsp. steigerwaltii DSM 16691GCA_001729725completencbi299766
66792Enterobacter hormaechei strain FDAARGOS 1434158836.1172completepatric158836
66792Enterobacter hormaechei subsp. steigerwaltii strain DSM 16691299766.39completepatric299766
66792Enterobacter hormaechei steigerwaltii DSM 166912721755484completeimg299766

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno65.185no
gram-positiveno98.059no
anaerobicno96.113yes
aerobicyes83.596no
halophileno86.416no
spore-formingno93.462no
thermophileno99.337yes
glucose-utilyes95.334no
motileyes88.473no
glucose-fermentyes93.644yes

External links

@ref: 22396

culture collection no.: DSM 16691, CCM 8661, CCUG 53904, CIP 108489

straininfo link

  • @ref: 89563
  • straininfo: 138307

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
22396Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-16691Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 16691)
41957Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/6116
60072Curators of the CCUGhttps://www.ccug.se/strain?id=53904Culture Collection University of Gothenburg (CCUG) (CCUG 53904)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68368Automatically annotated from API 20E
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
89563Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID138307.1
119968Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108489Collection of Institut Pasteur (CIP 108489)