Strain identifier

BacDive ID: 13110

Type strain: Yes

Species: Pseudomonas gessardii

Strain history: CIP <- 1998, D. Izard, Fac. Med. Henri Warembourg, Lille, France: strain CFML 95-251

NCBI tax ID(s): 78544 (species)

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General

@ref: 6793

BacDive-ID: 13110

DSM-Number: 17152

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, rod-shaped

description: Pseudomonas gessardii CCUG 43164 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from mineral water.

NCBI tax id

  • NCBI tax id: 78544
  • Matching level: species

strain history

@refhistory
6793<- CCM <- CCUG <- CIP <- D. Izard
119610CIP <- 1998, D. Izard, Fac. Med. Henri Warembourg, Lille, France: strain CFML 95-251

doi: 10.13145/bacdive13110.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas gessardii
  • full scientific name: Pseudomonas gessardii Verhille et al. 1999

@ref: 6793

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas gessardii

full scientific name: Pseudomonas gessardii Verhille et al. 1999 emend. van den Beld et al. 2016

type strain: yes

Morphology

cell morphology

  • @ref: 119610
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: no

pigmentation

  • @ref: 119610
  • production: no
  • name: Pyocyanin

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6793CASO AGAR (MERCK 105458) (DSMZ Medium 220)yeshttps://mediadive.dsmz.de/medium/220Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
42005MEDIUM 29- Brain heart agaryesDistilled water make up to (1000.000 ml);Brain heart infusion agar (52.000 g)
119610CIP Medium 29yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=29
119610CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
6793positivegrowth30mesophilic
42005positivegrowth30mesophilic
55703positivegrowth30mesophilic
119610positivegrowth5-30
119610nogrowth37mesophilic
119610nogrowth41thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
55703aerobe
119610obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
119610NaClpositivegrowth0-6 %
119610NaClnogrowth8 %
119610NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate+assimilation
6836927689decanoate+assimilation
6836924265gluconate+assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine+assimilation
6836916899D-mannitol+assimilation
6836916024D-mannose+assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose+assimilation
683695291gelatin+hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine+hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate+reduction
11961016947citrate+carbon source
1196104853esculin-hydrolysis
11961017632nitrate+reduction
11961016301nitrite+reduction
11961017632nitrate-respiration

antibiotic resistance

  • @ref: 119610
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleno
11961035581indoleno

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369cytochrome oxidase+1.9.3.1
68369gelatinase+
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase+3.5.3.6
119610oxidase+
119610beta-galactosidase-3.2.1.23
119610alcohol dehydrogenase+1.1.1.1
119610gelatinase+/-
119610amylase-
119610DNase-
119610caseinase+3.4.21.50
119610catalase+1.11.1.6
119610tween esterase-
119610lecithinase+
119610lipase-
119610lysine decarboxylase-4.1.1.18
119610ornithine decarboxylase-4.1.1.17
119610tryptophan deaminase-
119610urease-3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    55703C12:02.812
    55703C14:0114
    55703C15:01.615
    55703C16:025.516
    55703C17:00.717
    55703C18:00.318
    55703C10:0 3OH2.811.423
    55703C12:0 2OH6.513.178
    55703C12:0 3OH4.413.455
    55703C15:0 ISO 3OH2.216.135
    55703C16:1 ω7c26.515.819
    55703C17:0 CYCLO1316.888
    55703C18:1 ω7c /12t/9t5.917.824
    55703C18:2 ω6,9c/C18:0 ANTE0.417.724
    55703Unidentified0.310.469
    55703Unidentified1.410.662
    55703Unidentified0.712.268
    55703Unidentified316.297
    55703Unidentified0.817.2
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119610--++-++---++----+---

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
6793+--+--+-+-+++-+++++-+
6793+--+--+-+-+++-+++++-+

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
119610+++++--------------+------+++--+++---+--+--------++--+--+-+++---++++---+++-+++++++-----+++++++++++-

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentsampling dateisolation date
6793mineral waterFranceFRAEurope
55703Mineral watersFranceFRAEurope1995
119610Environment, Mineral waters1995

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic

taxonmaps

  • @ref: 69479
  • File name: preview.99_199.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_23;96_61;97_89;98_98;99_199&stattab=map
  • Last taxonomy: Pseudomonas
  • 16S sequence: AF074384
  • Sequence Identity:
  • Total samples: 8172
  • soil counts: 874
  • aquatic counts: 1631
  • animal counts: 3216
  • plant counts: 2451

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
67931Risk group (German classification)
1196101Risk group (French classification)

Sequence information

16S sequences

  • @ref: 6793
  • description: Pseudomonas gessardii 16S ribosomal RNA gene, complete sequence
  • accession: AF074384
  • length: 1516
  • database: ena
  • NCBI tax ID: 78544

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Pseudomonas gessardii DSM 17152GCA_001983165contigncbi78544
66792Pseudomonas gessardii DSM 17152GCA_009671285contigncbi78544
66792Pseudomonas gessardii strain DSM 1715278544.5wgspatric78544
66792Pseudomonas gessardii strain DSM 1715278544.11wgspatric78544
66792Pseudomonas gessardii DSM 171522917203564draftimg78544

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes89.451no
flagellatedyes83.823no
gram-positiveno98.2no
anaerobicno98.372yes
halophileno91.493no
spore-formingno95.286no
glucose-utilyes94.242yes
aerobicyes93.298yes
thermophileno99.872no
glucose-fermentno89.338yes

External links

@ref: 6793

culture collection no.: CCUG 43164, DSM 17152, CCM 4986, CFML 95-251, CIP 105469

straininfo link

  • @ref: 82318
  • straininfo: 69801

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny10555337Pseudomonas gessardii sp. nov. and Pseudomonas migulae sp. nov., two new species isolated from natural mineral waters.Verhille S, Baida N, Dabboussi F, Hamze M, Izard D, Leclerc HInt J Syst Bacteriol10.1099/00207713-49-4-15591999Bacterial Typing Techniques, Base Composition, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Mineral Waters/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, Polymerase Chain Reaction, Pseudomonas/*classification/genetics/isolation & purification, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Water MicrobiologyGenetics
Phylogeny12361251Pseudomonas grimontii sp. nov.Baida N, Yazourh A, Singer E, Izard DInt J Syst Evol Microbiol10.1099/00207713-52-5-14972002DNA, Bacterial/genetics, Fresh Water/microbiology, Microscopy, Electron, Molecular Sequence Data, Phylogeny, Pseudomonas/*classification/genetics/isolation & purification/metabolism, Terminology as TopicGenetics
Phylogeny20232682Isolation, identification and characterization of a new lipolytic pseudomonas sp., strain AHD-1, from Tunisian soil.Fendri I, Chaari A, Dhouib A, Jlassi B, Abousalham A, Carriere F, Sayadi S, Abdelkafi SEnviron Technol10.1080/095933309033699942010Lipid Metabolism/*physiology, Lipolysis/*physiology, Pseudomonas/*classification/isolation & purification/*physiology, *Soil Microbiology, Species Specificity, TunisiaMetabolism
Phylogeny27259517Possible misidentification of species in the Pseudomonas fluorescens lineage as Burkholderia pseudomallei and Francisella tularensis, and emended descriptions of Pseudomonas brenneri,Pseudomonas gessardii and Pseudomonas proteolytica.van den Beld MJC, Reinders E, Notermans DW, Reubsaet FAGInt J Syst Evol Microbiol10.1099/ijsem.0.0012062016Bacterial Typing Techniques, Burkholderia pseudomallei/*classification/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Francisella tularensis/*classification/isolation & purification, Netherlands, *Phylogeny, Pseudomonas/*classification/isolation & purification, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Species Specificity, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, *Water Microbiology, Water SupplyEnzymology
Phylogeny28141500Pseudomonas lactis sp. nov. and Pseudomonas paralactis sp. nov., isolated from bovine raw milk.von Neubeck M, Huptas C, Gluck C, Krewinkel M, Stoeckel M, Stressler T, Fischer L, Hinrichs J, Scherer S, Wenning MInt J Syst Evol Microbiol10.1099/ijsem.0.0018362017Animals, Bacterial Typing Techniques, Base Composition, Cattle, DNA, Bacterial/genetics, Fatty Acids/chemistry, Milk/*microbiology, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Pseudomonas/*classification/genetics/isolation & purification, Quinones/chemistry, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNATranscriptome
Phylogeny34309505Pseudomonas lactucae sp. nov., a pathogen causing bacterial rot of lettuce in Japan.Sawada H, Fujikawa T, Satou MInt J Syst Evol Microbiol10.1099/ijsem.0.0049172021Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Genes, Bacterial, Japan, Lettuce/*microbiology, Nucleic Acid Hybridization, *Phylogeny, Plant Diseases/*microbiology, Pseudomonas/*classification/isolation & purification, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNATranscriptome

Reference

@idauthorscataloguedoi/urltitle
6793Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17152)https://www.dsmz.de/collection/catalogue/details/culture/DSM-17152
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
42005Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17492
55703Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 43164)https://www.ccug.se/strain?id=43164
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
82318Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID69801.1StrainInfo: A central database for resolving microbial strain identifiers
119610Curators of the CIPCollection of Institut Pasteur (CIP 105469)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105469