Strain identifier
BacDive ID: 13103
Type strain:
Species: Pseudomonas costantinii
Strain Designation: PS3a, PS 3a
Strain history: CIP <- 2003 CFBP <- P. Münsch: strain PS 3a
NCBI tax ID(s): 168469 (species)
General
@ref: 6487
BacDive-ID: 13103
DSM-Number: 16734
keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped, plant pathogen
description: Pseudomonas costantinii PS3a is an aerobe, mesophilic, Gram-negative plant pathogen that was isolated from Agaricus bisporus.
NCBI tax id
- NCBI tax id: 168469
- Matching level: species
strain history
@ref | history |
---|---|
6487 | <- CFBP <- P. Munsch; PS3a |
118764 | CIP <- 2003 CFBP <- P. Münsch: strain PS 3a |
doi: 10.13145/bacdive13103.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Pseudomonadales
- family: Pseudomonadaceae
- genus: Pseudomonas
- species: Pseudomonas costantinii
- full scientific name: Pseudomonas costantinii Munsch et al. 2002
@ref: 6487
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Pseudomonadales
family: Pseudomonadaceae
genus: Pseudomonas
species: Pseudomonas costantinii
full scientific name: Pseudomonas costantinii Munsch et al. 2002
strain designation: PS3a, PS 3a
type strain: yes
Morphology
cell morphology
- @ref: 118764
- gram stain: negative
- cell shape: rod-shaped
- motility: yes
pigmentation
- @ref: 118764
- production: no
- name: Pyocyanin
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
6487 | YPGA (DSMZ Medium 1015) | yes | https://mediadive.dsmz.de/medium/1015 | Name: YPGA (DSMZ Medium 1015) Composition: Agar 15.0 g/l Glucose 7.0 g/l Bacto peptone 7.0 g/l Yeast extract 7.0 g/l Distilled water |
39948 | MEDIUM 72- for trypto casein soja agar | yes | Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g) | |
118764 | CIP Medium 72 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
6487 | positive | growth | 28 | mesophilic |
39948 | positive | growth | 30 | mesophilic |
59230 | positive | growth | 30 | mesophilic |
118764 | positive | growth | 5-30 | |
118764 | no | growth | 37 | mesophilic |
118764 | no | growth | 41 | thermophilic |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
59230 | aerobe |
118764 | obligate aerobe |
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
118764 | NaCl | positive | growth | 0-2 % |
118764 | NaCl | no | growth | 4 % |
118764 | NaCl | no | growth | 6 % |
118764 | NaCl | no | growth | 8 % |
118764 | NaCl | no | growth | 10 % |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
68369 | 25115 | malate | + | assimilation |
68369 | 17128 | adipate | - | assimilation |
68369 | 27689 | decanoate | + | assimilation |
68369 | 24265 | gluconate | + | assimilation |
68369 | 17306 | maltose | - | assimilation |
68369 | 59640 | N-acetylglucosamine | + | assimilation |
68369 | 16899 | D-mannitol | + | assimilation |
68369 | 16024 | D-mannose | + | assimilation |
68369 | 30849 | L-arabinose | + | assimilation |
68369 | 17634 | D-glucose | + | assimilation |
68369 | 5291 | gelatin | + | hydrolysis |
68369 | 4853 | esculin | - | hydrolysis |
68369 | 16199 | urea | - | hydrolysis |
68369 | 29016 | arginine | + | hydrolysis |
68369 | 17634 | D-glucose | - | fermentation |
68369 | 27897 | tryptophan | - | energy source |
68369 | 17632 | nitrate | - | reduction |
118764 | 16947 | citrate | + | carbon source |
118764 | 4853 | esculin | - | hydrolysis |
118764 | 17632 | nitrate | - | reduction |
118764 | 16301 | nitrite | - | reduction |
118764 | 17632 | nitrate | - | respiration |
antibiotic resistance
- @ref: 118764
- metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
- is antibiotic: yes
- is sensitive: no
- is resistant: yes
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
68369 | 35581 | indole | no |
118764 | 35581 | indole | no |
metabolite tests
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
@ref | value | activity | ec |
---|---|---|---|
68369 | cytochrome oxidase | + | 1.9.3.1 |
68369 | gelatinase | + | |
68369 | beta-glucosidase | - | 3.2.1.21 |
68369 | urease | - | 3.5.1.5 |
68369 | arginine dihydrolase | + | 3.5.3.6 |
118764 | oxidase | + | |
118764 | beta-galactosidase | - | 3.2.1.23 |
118764 | alcohol dehydrogenase | - | 1.1.1.1 |
118764 | gelatinase | - | |
118764 | amylase | - | |
118764 | DNase | - | |
118764 | caseinase | - | 3.4.21.50 |
118764 | catalase | + | 1.11.1.6 |
118764 | tween esterase | + | |
118764 | lecithinase | - | |
118764 | lipase | - | |
118764 | lysine decarboxylase | - | 4.1.1.18 |
118764 | ornithine decarboxylase | - | 4.1.1.17 |
118764 | protease | - | |
118764 | tryptophan deaminase | - | |
118764 | urease | - | 3.5.1.5 |
68382 | alkaline phosphatase | - | 3.1.3.1 |
68382 | esterase (C 4) | + | |
68382 | esterase lipase (C 8) | + | |
68382 | lipase (C 14) | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | valine arylamidase | - | |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | trypsin | + | 3.4.21.4 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
118764 | - | - | + | + | - | + | - | - | + | - | + | + | - | - | - | - | - | - | - | - |
API 20NE
@ref | NO3 | TRP | GLU_ Ferm | ADH Arg | URE | ESC | GEL | PNPG | GLU_ Assim | ARA | MNE | MAN | NAG | MAL | GNT | CAP | ADI | MLT | CIT | PAC | OX |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
6487 | - | - | - | + | - | - | + | - | + | + | + | + | + | - | + | + | - | + | + | - | + |
API biotype100
@ref | GLU | FRU | GAL | TRE | MNE | SBE | MEL | SAC | RAF | MTE | MAL | LAC | LTE | MbGa | MaGa | CEL | GEN | MbGu | ESC | RIB | ARA | XYL | PLE | RHA | FUC | MLZ | DARL | LARL | XLT | DUL | TAG | GLY | INO | MAN | MTL | TUR | SOR | ADO | HBG | LYX | ERY | MDG | 3MDG | SAT | MUC | LTAT | DTAT | MTAT | DMLT | LMLT | CATE | TATE | TTE | CIT | GRT | GAT | 2KG | 5KG | TRY | NAG | GNT | PAC | PAT | pOBE | QAT | GTE | mOBE | BAT | PPAT | CMT | TGE | BET | PCE | ABT | HIN | LAT | CAP | CYT | HIS | SUC | FUM | GRE | GYT | AVT | ETN | TTN | GLN | ITA | 3OBU | APT | GTT | PRO | DALA | LALA | SER | MNT | PROP | TYR | 2KT |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
118764 | + | + | + | + | + | - | - | + | - | - | - | - | - | - | - | - | - | - | - | + | + | + | - | - | - | - | + | + | + | - | - | + | + | + | - | - | + | + | - | - | + | - | - | + | + | - | + | + | - | + | + | + | - | + | + | + | + | + | - | + | + | - | + | + | + | - | - | + | - | - | - | + | + | + | - | + | + | + | - | + | + | + | + | - | - | - | + | + | + | + | + | + | + | + | + | + | + | + | + |
Isolation, sampling and environmental information
isolation
@ref | sample type | host species | country | origin.country | continent | isolation date |
---|---|---|---|---|---|---|
6487 | Agaricus bisporus | Agaricus bisporus | Finland | FIN | Europe | |
59230 | Agaricus bisporus | Finland | FIN | Europe | ||
118764 | Agaricus bisporus | Finland | FIN | Europe | 1997 |
isolation source categories
- Cat1: #Host
- Cat2: #Fungi
- Cat3: #Mushroom
taxonmaps
- @ref: 69479
- File name: preview.99_168.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_23;96_61;97_89;98_98;99_168&stattab=map
- Last taxonomy: Pseudomonas
- 16S sequence: AF374472
- Sequence Identity:
- Total samples: 5463
- soil counts: 555
- aquatic counts: 976
- animal counts: 2173
- plant counts: 1759
Safety information
risk assessment
@ref | pathogenicity plant | biosafety level | biosafety level comment |
---|---|---|---|
6487 | yes | 1 | Risk group (German classification) |
118764 | 1 | Risk group (French classification) |
Sequence information
16S sequences
- @ref: 6487
- description: Pseudomonas costantinii 16S ribosomal RNA gene, partial sequence
- accession: AF374472
- length: 1492
- database: ena
- NCBI tax ID: 168469
GC content
- @ref: 6487
- GC-content: 57.5
External links
@ref: 6487
culture collection no.: DSM 16734, CFBP 5705, HAMBI 2444, CCUG 51520, CIP 107803
straininfo link
- @ref: 82312
- straininfo: 87879
literature
- topic: Phylogeny
- Pubmed-ID: 12508856
- title: Pseudomonas costantinii sp. nov., another causal agent of brown blotch disease, isolated from cultivated mushroom sporophores in Finland.
- authors: Munsch P, Alatossava T, Marttinen N, Meyer JM, Christen R, Gardan L
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/00207713-52-6-1973
- year: 2002
- mesh: Agaricus, Base Composition, Base Sequence, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Fatty Acids/analysis, Finland, Genes, Bacterial, Microscopy, Electron, Molecular Sequence Data, Phenotype, Phylogeny, Pseudomonas/classification/genetics/*isolation & purification/*pathogenicity, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Species Specificity, Spores, Fungal, Virulence
- topic2: Genetics
Reference
@id | authors | catalogue | doi/url | title |
---|---|---|---|---|
6487 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 16734) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-16734 | |
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
39948 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/5278 | ||
59230 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 51520) | https://www.ccug.se/strain?id=51520 | |
68369 | Automatically annotated from API 20NE | |||
68382 | Automatically annotated from API zym | |||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |
82312 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID87879.1 | StrainInfo: A central database for resolving microbial strain identifiers | |
118764 | Curators of the CIP | Collection of Institut Pasteur (CIP 107803) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107803 |