Strain identifier

BacDive ID: 13103

Type strain: Yes

Species: Pseudomonas costantinii

Strain Designation: PS3a, PS 3a

Strain history: CIP <- 2003 CFBP <- P. Münsch: strain PS 3a

NCBI tax ID(s): 168469 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 6487

BacDive-ID: 13103

DSM-Number: 16734

keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped, plant pathogen

description: Pseudomonas costantinii PS3a is an aerobe, mesophilic, Gram-negative plant pathogen that was isolated from Agaricus bisporus.

NCBI tax id

  • NCBI tax id: 168469
  • Matching level: species

strain history

@refhistory
6487<- CFBP <- P. Munsch; PS3a
118764CIP <- 2003 CFBP <- P. Münsch: strain PS 3a

doi: 10.13145/bacdive13103.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas costantinii
  • full scientific name: Pseudomonas costantinii Munsch et al. 2002

@ref: 6487

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas costantinii

full scientific name: Pseudomonas costantinii Munsch et al. 2002

strain designation: PS3a, PS 3a

type strain: yes

Morphology

cell morphology

  • @ref: 118764
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

pigmentation

  • @ref: 118764
  • production: no
  • name: Pyocyanin

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6487YPGA (DSMZ Medium 1015)yeshttps://mediadive.dsmz.de/medium/1015Name: YPGA (DSMZ Medium 1015) Composition: Agar 15.0 g/l Glucose 7.0 g/l Bacto peptone 7.0 g/l Yeast extract 7.0 g/l Distilled water
39948MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
118764CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
6487positivegrowth28mesophilic
39948positivegrowth30mesophilic
59230positivegrowth30mesophilic
118764positivegrowth5-30
118764nogrowth37mesophilic
118764nogrowth41thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
59230aerobe
118764obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
118764NaClpositivegrowth0-2 %
118764NaClnogrowth4 %
118764NaClnogrowth6 %
118764NaClnogrowth8 %
118764NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate-assimilation
6836927689decanoate+assimilation
6836924265gluconate+assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine+assimilation
6836916899D-mannitol+assimilation
6836916024D-mannose+assimilation
6836930849L-arabinose+assimilation
6836917634D-glucose+assimilation
683695291gelatin+hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine+hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction
11876416947citrate+carbon source
1187644853esculin-hydrolysis
11876417632nitrate-reduction
11876416301nitrite-reduction
11876417632nitrate-respiration

antibiotic resistance

  • @ref: 118764
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleno
11876435581indoleno

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369cytochrome oxidase+1.9.3.1
68369gelatinase+
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase+3.5.3.6
118764oxidase+
118764beta-galactosidase-3.2.1.23
118764alcohol dehydrogenase-1.1.1.1
118764gelatinase-
118764amylase-
118764DNase-
118764caseinase-3.4.21.50
118764catalase+1.11.1.6
118764tween esterase+
118764lecithinase-
118764lipase-
118764lysine decarboxylase-4.1.1.18
118764ornithine decarboxylase-4.1.1.17
118764protease-
118764tryptophan deaminase-
118764urease-3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
118764--++-+--+-++--------

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
6487---+--+-+++++-++-++-+

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
118764+++++--+-----------+++----+++--+++--++--+--++-++-+++-+++++-++-+++--+---+++-+++-++++---+++++++++++++

Isolation, sampling and environmental information

isolation

@refsample typehost speciescountryorigin.countrycontinentisolation date
6487Agaricus bisporusAgaricus bisporusFinlandFINEurope
59230Agaricus bisporusFinlandFINEurope
118764Agaricus bisporusFinlandFINEurope1997

isolation source categories

  • Cat1: #Host
  • Cat2: #Fungi
  • Cat3: #Mushroom

taxonmaps

  • @ref: 69479
  • File name: preview.99_168.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_23;96_61;97_89;98_98;99_168&stattab=map
  • Last taxonomy: Pseudomonas
  • 16S sequence: AF374472
  • Sequence Identity:
  • Total samples: 5463
  • soil counts: 555
  • aquatic counts: 976
  • animal counts: 2173
  • plant counts: 1759

Safety information

risk assessment

@refpathogenicity plantbiosafety levelbiosafety level comment
6487yes1Risk group (German classification)
1187641Risk group (French classification)

Sequence information

16S sequences

  • @ref: 6487
  • description: Pseudomonas costantinii 16S ribosomal RNA gene, partial sequence
  • accession: AF374472
  • length: 1492
  • database: ena
  • NCBI tax ID: 168469

GC content

  • @ref: 6487
  • GC-content: 57.5

External links

@ref: 6487

culture collection no.: DSM 16734, CFBP 5705, HAMBI 2444, CCUG 51520, CIP 107803

straininfo link

  • @ref: 82312
  • straininfo: 87879

literature

  • topic: Phylogeny
  • Pubmed-ID: 12508856
  • title: Pseudomonas costantinii sp. nov., another causal agent of brown blotch disease, isolated from cultivated mushroom sporophores in Finland.
  • authors: Munsch P, Alatossava T, Marttinen N, Meyer JM, Christen R, Gardan L
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/00207713-52-6-1973
  • year: 2002
  • mesh: Agaricus, Base Composition, Base Sequence, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Fatty Acids/analysis, Finland, Genes, Bacterial, Microscopy, Electron, Molecular Sequence Data, Phenotype, Phylogeny, Pseudomonas/classification/genetics/*isolation & purification/*pathogenicity, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Species Specificity, Spores, Fungal, Virulence
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitle
6487Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 16734)https://www.dsmz.de/collection/catalogue/details/culture/DSM-16734
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
39948Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5278
59230Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 51520)https://www.ccug.se/strain?id=51520
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
82312Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID87879.1StrainInfo: A central database for resolving microbial strain identifiers
118764Curators of the CIPCollection of Institut Pasteur (CIP 107803)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107803