Strain identifier

BacDive ID: 130998

Type strain: No

Species: Pseudomonas aeruginosa

Strain Designation: W

Strain history: CIP <- 2016, CCUG

NCBI tax ID(s): 287 (species)

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General

@ref: 22290

BacDive-ID: 130998

DSM-Number: 101013

keywords: genome sequence, Bacteria, aerobe, mesophilic, Gram-negative, human pathogen

description: Pseudomonas aeruginosa W is an aerobe, mesophilic, Gram-negative human pathogen that was isolated from urine.

NCBI tax id

  • NCBI tax id: 287
  • Matching level: species

strain history

@refhistory
22290<- NCTC; <- PHLS; <- G. A. Codd, Newcastle General Hospital, England; W
116093CIP <- 2016, CCUG

doi: 10.13145/bacdive130998.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas aeruginosa
  • full scientific name: Pseudomonas aeruginosa (Schroeter 1872) Migula 1900 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Bacterium aeruginosum

@ref: 22290

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas aeruginosa

full scientific name: Pseudomonas aeruginosa (Schroeter 1872) Migula 1900

strain designation: W

type strain: no

Morphology

cell morphology

@refgram stainconfidencemotilitycell shape
69480negative99.994
6948098.898yes
116093negativeyesrod-shaped

Culture and growth conditions

culture medium

@refnamegrowthlink
22290TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium92.pdf
22290COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium693.pdf
116093CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
22290positivegrowth30mesophilic
63429positivegrowth30mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 63429
  • oxygen tolerance: aerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.967

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate+assimilation
6836927689decanoate+assimilation
6836924265gluconate+assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine+assimilation
6836916899D-mannitol+assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose+assimilation
683695291gelatin+hydrolysis
683694853esculin-hydrolysis
6836929016arginine+hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate+reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369cytochrome oxidase+1.9.3.1
68369gelatinase+
68369beta-glucosidase-3.2.1.21
68369arginine dihydrolase+3.5.3.6

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    63429C12:04.712
    63429C14:00.714
    63429C16:022.216
    63429C10:0 3OH611.423
    63429C12:0 2OH9.213.178
    63429C12:0 3OH7.813.455
    63429C16:1 ω7c19.915.819
    63429C18:1 ω7c /12t/9t28.817.824
    63429C19:0 CYCLO ω8c0.718.9
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
22290+--++/--+-+--++-+++++-+
63429+--+--+-+--++-+++++-+
22290+--+--+-+--++-+++++-+

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitude
22290urineNewcastleUnited KingdomGBREurope54.9783-1.6178
116093Human, UrineNewcastleUnited KingdomGBREurope

isolation source categories

  • Cat1: #Host Body Product
  • Cat2: #Fluids
  • Cat3: #Urine

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
22290yesyes2Risk group (German classification)
1160932Risk group (French classification)

Sequence information

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Pseudomonas aeruginosa NCTC12951GCA_900706975contigncbi287
66792Pseudomonas aeruginosa strain NCTC12951287.9429wgspatric287

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
gram-positiveno98.094no
anaerobicno98.682yes
halophileno93.983no
spore-formingno92.556no
glucose-utilyes88.792yes
motileyes87.587no
flagellatedyes78.195no
aerobicyes93.201no
thermophileno99.837yes
glucose-fermentno89.357yes

External links

@ref: 22290

culture collection no.: DSM 101013, NCTC 12951, WDCM 00207, CCUG 67716, CECT 8735, CIP 111242

straininfo link

@refstraininfo
89469395120
89470399369

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
22290Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-101013Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 101013)
63429Curators of the CCUGhttps://www.ccug.se/strain?id=67716Culture Collection University of Gothenburg (CCUG) (CCUG 67716)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68369Automatically annotated from API 20NE
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
89469Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID395120.1
89470Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID399369.1
116093Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20111242Collection of Institut Pasteur (CIP 111242)