Strain identifier

BacDive ID: 130972

Type strain: Yes

Species: Anseongella ginsenosidimutans

Strain history: <- ST Lee, KAIST

NCBI tax ID(s): 496056 (species)

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General

@ref: 22264

BacDive-ID: 130972

DSM-Number: 21100

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, Gram-negative, rod-shaped, colony-forming

description: Anseongella ginsenosidimutans DSM 21100 is an obligate aerobe, Gram-negative, rod-shaped bacterium that forms circular colonies and was isolated from soil of a ginseng field.

NCBI tax id

  • NCBI tax id: 496056
  • Matching level: species

strain history

@refhistory
22264<- S.-T. Lee, KAIST <- W.-T. Im; Gsoil524
67771<- ST Lee, KAIST

doi: 10.13145/bacdive130972.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Sphingobacteriia
  • order: Sphingobacteriales
  • family: Sphingobacteriaceae
  • genus: Anseongella
  • species: Anseongella ginsenosidimutans
  • full scientific name: Anseongella ginsenosidimutans Siddiqi et al. 2016

@ref: 22264

domain: Bacteria

phylum: Bacteroidetes

class: Sphingobacteriia

order: Sphingobacteriales

family: Sphingobacteriaceae

genus: Anseongella

species: Anseongella ginsenosidimutans

full scientific name: Anseongella ginsenosidimutans Siddiqi et al. 2016

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
43700negative1.5-2.5 µm0.4-0.6 µmrod-shaped
67771rod-shaped
67771negative
69480no90.529
69480negative99.99

colony morphology

  • @ref: 43700
  • colony color: Milky-white
  • colony shape: circular
  • incubation period: 2 days
  • medium used: R2A agar

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
43700Reasoner's 2A agar (R2A)yes
22264R2A MEDIUM (DSMZ Medium 830)yeshttps://mediadive.dsmz.de/medium/830Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
43700positivegrowth15-37
43700positiveoptimum30mesophilic
22264positivegrowth28mesophilic
67771positivegrowth30mesophilic

culture pH

@refabilitytypepHPH range
43700positivegrowth5-10alkaliphile
43700positiveoptimum6-7

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
43700obligate aerobe
67771aerobe

spore formation

@refspore formationconfidence
43700no
69481no100
69480no99.993

halophily

  • @ref: 43700
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-7 %(w/v)

observation

  • @ref: 67771
  • observation: quinones: MK-7

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
43700167244-hydroxybutyrate-assimilation
4370017128adipate-assimilation
4370027689decanoate-assimilation
4370016988D-ribose-assimilation
4370017924D-sorbitol-assimilation
4370028087glycogen-assimilation
4370016977L-alanine-assimilation
4370015971L-histidine-assimilation
4370017203L-proline-assimilation
4370017115L-serine-assimilation
4370025115malate-assimilation
4370017306maltose-assimilation
4370018401phenylacetate-assimilation
43700potassium 2-dehydro-D-gluconate-assimilation
43700potassium 5-dehydro-D-gluconate-assimilation
4370032032potassium gluconate-assimilation
4370017272propionate-assimilation
4370017814salicin-assimilation
4370053258sodium citrate-assimilation
4370017992sucrose-assimilation
4370031011valerate-assimilation
4370085146carboxymethylcellulose-hydrolysis
43700casein-hydrolysis
4370016991dna-hydrolysis
437005291gelatin-hydrolysis
4370028017starch-hydrolysis
4370053426tween 80-hydrolysis
4370016199urea-hydrolysis
4370037166xylan-hydrolysis
4370017632nitrate-reduction
43700370543-hydroxybutyrate+assimilation
4370022599arabinose+assimilation
4370017634D-glucose+assimilation
4370016024D-mannose+assimilation
4370017268myo-inositol+assimilation
4370017240itaconate+assimilation
4370018287L-fucose+assimilation
4370062345L-rhamnose+assimilation
4370029864mannitol+assimilation
4370028053melibiose+assimilation
43700506227N-acetylglucosamine+assimilation
4370017234glucose+fermentation
437004853esculin+hydrolysis

metabolite production

  • @ref: 43700
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
43700beta-glucosidase+3.2.1.21
43700beta-galactosidase+3.2.1.23
43700alkaline phosphatase+3.1.3.1
43700leucine arylamidase+3.4.11.1
43700valine arylamidase+
43700alpha-glucosidase+3.2.1.20
43700acid phosphatase+3.1.3.2
43700naphthol-AS-BI-phosphohydrolase+
43700arginine dihydrolase-3.5.3.6
43700lipase (C 14)-
43700urease-3.5.1.5
43700esterase (C 4)-
43700esterase Lipase (C 8)-
43700alpha-fucosidase-3.2.1.51
43700beta-glucuronidase-3.2.1.31
43700trypsin-3.4.21.4

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    43700C17:0 anteiso8.9
    43700C11:0 3OH14
    43700C12:0 aldehyde / Unknown1.3
    43700C14:02.1
    43700C16:03.8
    43700C16:1ω7c / C16:1ω6c0.6
    43700C11:0 iso6.9
    43700C13:0 iso0.6
    43700C14:0 iso4.5
    43700C15:0 iso29.7
    43700iso-C15:1F3
    43700C16:0 iso10.7
    43700C17:0 iso1.4
    43700C17:1 iso ω9c / C16:0 10-methyl10.2
  • type of FA analysis: whole cell analysis
  • incubation medium: R2A
  • agar/liquid: agar
  • incubation temperature: 30
  • incubation time: 2
  • incubation_oxygen: aerobic
  • software version: Sherlock 6.0
  • library/peak naming table: TSBA60
  • system: MIS MIDI
  • instrument: Hewlett Packard 6890

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
22264soil of a ginseng fieldPocheon provinceRepublic of KoreaKORAsia
43700The soil of a ginseng filedPocheon cityRepublic of KoreaKORAsia
67771From ginseng soil collected from Pocheon provinceRepublic of KoreaKORAsia

isolation source categories

Cat1Cat2Cat3
#Engineered#Agriculture#Field
#Environmental#Terrestrial#Soil
#Host#Plants#Herbaceous plants (Grass,Crops)

Safety information

risk assessment

  • @ref: 22264
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 22264
  • description: Anseongella ginsenosidimutans strain Gsoil 524 16S ribosomal RNA gene, partial sequence
  • accession: EU370954
  • length: 1457
  • database: ena
  • NCBI tax ID: 496056

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Anseongella ginsenosidimutans DSM 21100GCA_004346135contigncbi496056
66792Anseongella ginsenosidimutans strain DSM 21100496056.3wgspatric496056
66792Anseongella ginsenosidimutans DSM 211002784746790draftimg496056

GC content

  • @ref: 22264
  • GC-content: 51.9
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
gram-positiveno98.05yes
anaerobicno99.038yes
halophileno93.507no
spore-formingno93.815yes
glucose-utilyes87.222no
aerobicyes85.946no
thermophileno91.866no
motileno92.385no
flagellatedno98.278no
glucose-fermentno80.302yes

External links

@ref: 22264

culture collection no.: DSM 21100, KACC 14636, KCTC 22261, LMG 24494, Gsoil 524

straininfo link

  • @ref: 89447
  • straininfo: 400451

literature

  • topic: Phylogeny
  • Pubmed-ID: 26669622
  • title: Anseongella ginsenosidimutans gen. nov., sp. nov., isolated from soil cultivating ginseng.
  • authors: Siddiqi MZ, Liu Q, Kang MS, Kim MS, Im WT
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.000844
  • year: 2015

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
22264Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-21100Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21100)
43700Muhammad Zubair Siddiqi, Qingmei Liu, Myung-Suk Kang, Minseok S. Kim, Wan-Taek ImAnseongella ginsenosidimutans gen. nov., sp. nov., isolated from soil cultivating ginseng10.1099/ijsem.0.000844IJSEM 66: 1125-1130 201626669622
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar SchomburgBRENDA, the ELIXIR core data resource in 2021: new developments and updates10.1093/nar/gkaa1025Nucleic Acids Res. 49: D498-D508 202033211880
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
89447Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID400451.1