Strain identifier

BacDive ID: 13097

Type strain: Yes

Species: Pseudomonas koreensis

Strain Designation: Ps 9-14

Strain history: CIP <- 2005, KACC <- Nat. Inst. Agricult. Biotechnol., Korea: strain Ps 9-14

NCBI tax ID(s): 198620 (species)

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General

@ref: 6520

BacDive-ID: 13097

DSM-Number: 16610

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Pseudomonas koreensis Ps 9-14 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from agricultural soil.

NCBI tax id

  • NCBI tax id: 198620
  • Matching level: species

strain history

@refhistory
6520<- S. W. Kwon; Ps 9-14
67770LMG 21318 <-- S.-W. Kwon Ps 9-14.
120483CIP <- 2005, KACC <- Nat. Inst. Agricult. Biotechnol., Korea: strain Ps 9-14

doi: 10.13145/bacdive13097.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas koreensis
  • full scientific name: Pseudomonas koreensis Kwon et al. 2003

@ref: 6520

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas koreensis

full scientific name: Pseudomonas koreensis Kwon et al. 2003

strain designation: Ps 9-14

type strain: yes

Morphology

cell morphology

  • @ref: 120483
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

pigmentation

  • @ref: 120483
  • production: no
  • name: Pyocyanin

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6520CASO AGAR (MERCK 105458) (DSMZ Medium 220)yeshttps://mediadive.dsmz.de/medium/220Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
34001MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
120483CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
6520positivegrowth30mesophilic
34001positivegrowth30mesophilic
59229positivegrowth30mesophilic
67770positivegrowth30mesophilic
120483positivegrowth5-37
120483nogrowth41thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
59229aerobe
120483obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
120483NaClpositivegrowth0-6 %
120483NaClnogrowth8 %
120483NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate-assimilation
6836927689decanoate+assimilation
6836924265gluconate+assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine+assimilation
6836916899D-mannitol+assimilation
6836916024D-mannose+assimilation
6836930849L-arabinose+assimilation
6836917634D-glucose+assimilation
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine+hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction
12048316947citrate+carbon source
1204834853esculin-hydrolysis
12048317632nitrate-reduction
12048316301nitrite-reduction
12048317632nitrate-respiration

antibiotic resistance

  • @ref: 120483
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleno
12048335581indoleno

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369cytochrome oxidase+1.9.3.1
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase+3.5.3.6
120483oxidase+
120483beta-galactosidase-3.2.1.23
120483alcohol dehydrogenase+1.1.1.1
120483gelatinase-
120483amylase+
120483DNase-
120483caseinase-3.4.21.50
120483catalase+1.11.1.6
120483tween esterase-
120483lecithinase-
120483lipase-
120483lysine decarboxylase-4.1.1.18
120483ornithine decarboxylase-4.1.1.17
120483protease-
120483tryptophan deaminase-
120483urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120483-+++-+--+-++--------

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
6520---+--+++++++-++-++-+
6520---+----+++++-++-++-+

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
120483+++-+--------------++-----+----+-+---------++---++++-+--+--++-+++-----++++-++++++++++-+++++++++++++

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
6520agricultural soilregions Goesan, Samchok and UmsongRepublic of KoreaKORAsia
59229Soil,agriculturalRepublic of KoreaKORAsia
67770Soil
120483Environment, SoilUmsong regionRepublic of KoreaKORAsia2000

isolation source categories

Cat1Cat2Cat3
#Engineered#Agriculture
#Environmental#Terrestrial#Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_185.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_23;96_61;97_89;98_98;99_185&stattab=map
  • Last taxonomy: Pseudomonas
  • 16S sequence: AF468452
  • Sequence Identity:
  • Total samples: 2464
  • soil counts: 833
  • aquatic counts: 448
  • animal counts: 521
  • plant counts: 662

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
65201Risk group (German classification)
1204831Risk group (French classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: Pseudomonas sp. Ps 9-14 16S ribosomal RNA gene, partial sequence
  • accession: AF468452
  • length: 1455
  • database: ena
  • NCBI tax ID: 198620

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Pseudomonas koreensis JCM 14769GCA_014646955scaffoldncbi198620
66792Pseudomonas koreensis CCUG 51519GCA_008801535contigncbi198620
66792Pseudomonas koreensis strain CCUG 51519198620.78wgspatric198620
66792Pseudomonas koreensis strain DSM 16610198620.87wgspatric198620
66792Pseudomonas koreensis strain JCM 14769198620.93wgspatric198620
66792Pseudomonas koreensis LMG 213182636416187draftimg198620
67770Pseudomonas koreensis DSM 16610GCA_012986595contigncbi198620
66792Pseudomonas koreensis LMG 21318GCA_900101415chromosomencbi198620

GC content

  • @ref: 67770
  • GC-content: 60.7
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes92.733no
flagellatedyes82.412no
gram-positiveno98.209no
anaerobicno97.784yes
aerobicyes94.306yes
halophileno91.461no
spore-formingno94.579no
glucose-fermentno91.318yes
thermophileno99.884yes
glucose-utilyes94.646yes

External links

@ref: 6520

culture collection no.: DSM 16610, KACC 10848, LMG 21318, CCUG 51519, JCM 14769, CIP 108616

straininfo link

  • @ref: 82307
  • straininfo: 86038

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny12656147Pseudomonas koreensis sp. nov., Pseudomonas umsongensis sp. nov. and Pseudomonas jinjuensis sp. nov., novel species from farm soils in Korea.Kwon SW, Kim JS, Park IC, Yoon SH, Park DH, Lim CK, Go SJInt J Syst Evol Microbiol10.1099/ijs.0.02326-02003Agriculture, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Korea, Microscopy, Electron, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, Pseudomonas/*classification/genetics/*isolation & purification/metabolism, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, *Soil Microbiology, Species SpecificityGenetics
Phylogeny17082407Pseudomonas moraviensis sp. nov. and Pseudomonas vranovensis sp. nov., soil bacteria isolated on nitroaromatic compounds, and emended description of Pseudomonas asplenii.Tvrzova L, Schumann P, Sproer C, Sedlacek I, Pacova Z, Sedo O, Zdrahal Z, Steffen M, Lang EInt J Syst Evol Microbiol10.1099/ijs.0.63988-02006Amino Acids/metabolism, Bacterial Typing Techniques, Carbohydrate Metabolism, Czech Republic, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Genes, rRNA, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, Pseudomonas/*classification/cytology/*isolation & purification/physiology, RNA, Ribosomal, 16S/genetics, Ribotyping, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, *Soil MicrobiologyMetabolism
Phylogeny21642488Pseudomonas baetica sp. nov., a fish pathogen isolated from wedge sole, Dicologlossa cuneata (Moreau).Lopez JR, Dieguez AL, Doce A, De la Roca E, De la Herran R, Navas JI, Toranzo AE, Romalde JLInt J Syst Evol Microbiol10.1099/ijs.0.030601-02011Animals, Bacterial Typing Techniques, DNA, Bacterial/genetics, Fatty Acids/chemistry, Fish Diseases/microbiology, Flatfishes/*microbiology, Genes, Bacterial, Liver/microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, Pseudomonas/classification/genetics/*isolation & purification, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, SpainGenetics
Phylogeny27460204Pseudomonas kribbensis sp. nov., isolated from garden soils in Daejeon, Korea.Chang DH, Rhee MS, Kim JS, Lee Y, Park MY, Kim H, Lee SG, Kim BCAntonie Van Leeuwenhoek10.1007/s10482-016-0743-02016Base Composition, DNA, Bacterial, Gardens, Molecular Typing, Phylogeny, Pseudomonas/classification/genetics/*isolation & purification/ultrastructure, RNA, Bacterial, RNA, Ribosomal, 16S/genetics, Republic of Korea, *Soil MicrobiologyEnzymology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
6520Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 16610)https://www.dsmz.de/collection/catalogue/details/culture/DSM-16610
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
34001Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/6265
59229Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 51519)https://www.ccug.se/strain?id=51519
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
82307Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID86038.1StrainInfo: A central database for resolving microbial strain identifiers
120483Curators of the CIPCollection of Institut Pasteur (CIP 108616)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108616