Strain identifier

BacDive ID: 130954

Type strain: No

Species: Escherichia coli

Strain Designation: Mt1B1

Strain history: <- T. Clavel, TU Munich, Freising-Weihenstephan, Germany; Mt1B1

NCBI tax ID(s): 562 (species)

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General

@ref: 22246

BacDive-ID: 130954

DSM-Number: 28618

keywords: genome sequence, 16S sequence, Bacteria, mesophilic, human pathogen

description: Escherichia coli Mt1B1 is a mesophilic human pathogen that was isolated from ileal mucosa; TNFdeltaARE/+ C57BL/6 mouse.

NCBI tax id

  • NCBI tax id: 562
  • Matching level: species

strain history

  • @ref: 22246
  • history: <- T. Clavel, TU Munich, Freising-Weihenstephan, Germany; Mt1B1

doi: 10.13145/bacdive130954.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Enterobacteriaceae
  • genus: Escherichia
  • species: Escherichia coli
  • full scientific name: Escherichia coli (Migula 1895) Castellani and Chalmers 1919 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Bacillus coli

@ref: 22246

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacteriales, not validated

family: Enterobacteriaceae

genus: Escherichia

species: Escherichia coli

full scientific name: Escherichia coli (Migula 1895) Castellani and Chalmers 1919

strain designation: Mt1B1

type strain: no

Morphology

colony morphology

  • @ref: 22246
  • incubation period: 2-3 days

Culture and growth conditions

culture medium

  • @ref: 22246
  • name: LB (Luria-Bertani) MEDIUM (DSMZ Medium 381)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/381
  • composition: Name: LB (Luria-Bertani) MEDIUM (DSMZ Medium 381) Composition: Agar 20.0 g/l NaCl 10.0 g/l Tryptone 10.0 g/l Yeast extract 5.0 g/l Distilled water

culture temp

  • @ref: 22246
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836817632nitrate+reduction
6836830849L-arabinose+fermentation
6836827613amygdalin-fermentation
6836828053melibiose+fermentation
6836817992sucrose+fermentation
6836862345L-rhamnose+fermentation
6836830911sorbitol+fermentation
6836817268myo-inositol-fermentation
6836816899D-mannitol+fermentation
6836817634D-glucose+fermentation
683685291gelatin-hydrolysis
6836827897tryptophan+energy source
6836816199urea-hydrolysis
6836816947citrate-assimilation
6836818257ornithine+degradation
6836825094lysine+degradation
6836829016arginine-hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6836816301nitriteyes
6836835581indoleyes
6836816136hydrogen sulfideno

metabolite tests

  • @ref: 68368
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: +

enzymes

@refvalueactivityec
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase+4.1.1.17
68368lysine decarboxylase+4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase+3.2.1.23

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARANO2
22246+-++----+--++-++++-++
22246+-++----++/--++-++++-++
22246+-++----++-++-++++-++
22246+-++----++/--++-++++-++

Isolation, sampling and environmental information

isolation

  • @ref: 22246
  • sample type: ileal mucosa; TNFdeltaARE/+ C57BL/6 mouse
  • geographic location: Freising
  • country: Germany
  • origin.country: DEU
  • continent: Europe

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Muridae (Mouse/Rat)
#Host Body-Site#Gastrointestinal tract#Small intestine

taxonmaps

  • @ref: 69479
  • File name: preview.99_8.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_6;96_7;97_7;98_7;99_8&stattab=map
  • Last taxonomy: Enterobacteriaceae
  • 16S sequence: AM944637
  • Sequence Identity:
  • Total samples: 97630
  • soil counts: 2111
  • aquatic counts: 5613
  • animal counts: 88071
  • plant counts: 1835

Safety information

risk assessment

  • @ref: 22246
  • pathogenicity human: yes
  • pathogenicity animal: yes
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 22246
  • description: Escherichia coli partial 16S rRNA gene, strain Mt1B1
  • accession: AM944637
  • length: 1422
  • database: ena
  • NCBI tax ID: 562

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Escherichia coli Mt1B1GCA_003052625chromosomencbi562
66792Escherichia coli Mt1B1GCA_010206135contigncbi562
66792Escherichia coli strain Mt1B1562.29017completepatric562

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
gram-positiveno98.225no
anaerobicno94.983no
halophileno92.125no
spore-formingno94.087no
glucose-utilyes93.499no
aerobicyes83.199no
thermophileno99.31yes
motileyes90.1no
flagellatedno55.071no
glucose-fermentyes93.338yes

External links

@ref: 22246

culture collection no.: DSM 28618

straininfo link

  • @ref: 89431
  • straininfo: 397908

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
22246Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-28618Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 28618)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68368Automatically annotated from API 20E
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
89431Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID397908.1