Strain identifier
BacDive ID: 13095
Type strain:
Species: Pseudomonas proteolytica
Strain history: CIP <- 2004, S. Shivaji, CCMB, Hyderabad, India: strain CMS64
NCBI tax ID(s): 219574 (species)
General
@ref: 5843
BacDive-ID: 13095
DSM-Number: 15321
keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped
description: Pseudomonas proteolytica DSM 15321 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from cyanobacterial mat samples.
NCBI tax id
- NCBI tax id: 219574
- Matching level: species
strain history
@ref | history |
---|---|
5843 | <- S. Shivaji; CMS 64 |
42006 | 2004, S. Shivaji, CCMB, Hyderabad, India: strain CMS64 |
121968 | CIP <- 2004, S. Shivaji, CCMB, Hyderabad, India: strain CMS64 |
doi: 10.13145/bacdive13095.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Pseudomonadales
- family: Pseudomonadaceae
- genus: Pseudomonas
- species: Pseudomonas proteolytica
- full scientific name: Pseudomonas proteolytica Reddy et al. 2004
@ref: 5843
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Pseudomonadales
family: Pseudomonadaceae
genus: Pseudomonas
species: Pseudomonas proteolytica
full scientific name: Pseudomonas proteolytica Reddy et al. 2004 emend. van den Beld et al. 2016
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell shape | motility |
---|---|---|---|
30011 | negative | rod-shaped | yes |
121968 | negative | rod-shaped | yes |
pigmentation
@ref | production | name |
---|---|---|
30011 | no | |
121968 | no | Pyocyanin |
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
5843 | CASO AGAR (MERCK 105458) (DSMZ Medium 220) | yes | https://mediadive.dsmz.de/medium/220 | Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water |
42006 | MEDIUM 72- for trypto casein soja agar | yes | Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g) | |
121968 | CIP Medium 72 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
5843 | positive | growth | 28 | mesophilic |
30011 | positive | growth | 04-30 | |
30011 | positive | optimum | 33 | mesophilic |
42006 | positive | growth | 25 | mesophilic |
59225 | positive | growth | 30 | mesophilic |
121968 | positive | growth | 5-30 | |
121968 | no | growth | 37 | mesophilic |
121968 | no | growth | 41 | thermophilic |
culture pH
- @ref: 30011
- ability: positive
- type: optimum
- pH: 7
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
30011 | aerobe |
59225 | aerobe |
121968 | obligate aerobe |
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
30011 | NaCl | positive | growth | 0-3 % |
121968 | NaCl | positive | growth | 0-10 % |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
30011 | 30089 | acetate | + | carbon source |
30011 | 15963 | ribitol | + | carbon source |
30011 | 16449 | alanine | + | carbon source |
30011 | 29016 | arginine | + | carbon source |
30011 | 16947 | citrate | + | carbon source |
30011 | 28757 | fructose | + | carbon source |
30011 | 28260 | galactose | + | carbon source |
30011 | 5291 | gelatin | + | carbon source |
30011 | 17234 | glucose | + | carbon source |
30011 | 29987 | glutamate | + | carbon source |
30011 | 17754 | glycerol | + | carbon source |
30011 | 24996 | lactate | + | carbon source |
30011 | 29864 | mannitol | + | carbon source |
30011 | 37684 | mannose | + | carbon source |
30011 | 17268 | myo-inositol | + | carbon source |
30011 | 28044 | phenylalanine | + | carbon source |
30011 | 15361 | pyruvate | + | carbon source |
30011 | 33942 | ribose | + | carbon source |
30011 | 17822 | serine | + | carbon source |
30011 | 30911 | sorbitol | + | carbon source |
30011 | 27082 | trehalose | + | carbon source |
30011 | 16296 | D-tryptophan | + | carbon source |
30011 | 17632 | nitrate | + | reduction |
68369 | 25115 | malate | + | assimilation |
68369 | 17128 | adipate | + | assimilation |
68369 | 27689 | decanoate | + | assimilation |
68369 | 24265 | gluconate | + | assimilation |
68369 | 17306 | maltose | - | assimilation |
68369 | 59640 | N-acetylglucosamine | + | assimilation |
68369 | 16899 | D-mannitol | + | assimilation |
68369 | 16024 | D-mannose | + | assimilation |
68369 | 30849 | L-arabinose | - | assimilation |
68369 | 17634 | D-glucose | + | assimilation |
68369 | 5291 | gelatin | + | hydrolysis |
68369 | 4853 | esculin | - | hydrolysis |
68369 | 16199 | urea | - | hydrolysis |
68369 | 29016 | arginine | + | hydrolysis |
68369 | 17634 | D-glucose | - | fermentation |
68369 | 27897 | tryptophan | - | energy source |
68369 | 17632 | nitrate | + | reduction |
121968 | 16947 | citrate | + | carbon source |
121968 | 4853 | esculin | - | hydrolysis |
121968 | 17632 | nitrate | + | reduction |
121968 | 16301 | nitrite | + | reduction |
121968 | 17632 | nitrate | + | respiration |
antibiotic resistance
- @ref: 121968
- metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
- is antibiotic: yes
- is sensitive: no
- is resistant: yes
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
68369 | 35581 | indole | no |
121968 | 35581 | indole | no |
metabolite tests
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
@ref | value | activity | ec |
---|---|---|---|
30011 | catalase | + | 1.11.1.6 |
30011 | gelatinase | + | |
30011 | cytochrome oxidase | + | 1.9.3.1 |
68369 | cytochrome oxidase | + | 1.9.3.1 |
68369 | gelatinase | + | |
68369 | beta-glucosidase | - | 3.2.1.21 |
68369 | urease | - | 3.5.1.5 |
68369 | arginine dihydrolase | + | 3.5.3.6 |
121968 | oxidase | - | |
121968 | beta-galactosidase | - | 3.2.1.23 |
121968 | alcohol dehydrogenase | + | 1.1.1.1 |
121968 | gelatinase | +/- | |
121968 | amylase | - | |
121968 | DNase | + | |
121968 | caseinase | + | 3.4.21.50 |
121968 | catalase | + | 1.11.1.6 |
121968 | tween esterase | + | |
121968 | lecithinase | + | |
121968 | lipase | + | |
121968 | lysine decarboxylase | - | 4.1.1.18 |
121968 | ornithine decarboxylase | - | 4.1.1.17 |
121968 | protease | + | |
121968 | tryptophan deaminase | - | |
121968 | urease | - | 3.5.1.5 |
68382 | alkaline phosphatase | - | 3.1.3.1 |
68382 | esterase (C 4) | + | |
68382 | esterase lipase (C 8) | + | |
68382 | lipase (C 14) | + | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | valine arylamidase | + | |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | trypsin | + | 3.4.21.4 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
121968 | - | - | + | + | + | + | + | - | + | - | + | + | - | - | - | - | - | - | - | - |
API 20NE
@ref | NO3 | TRP | GLU_ Ferm | ADH Arg | URE | ESC | GEL | PNPG | GLU_ Assim | ARA | MNE | MAN | NAG | MAL | GNT | CAP | ADI | MLT | CIT | PAC | OX |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
5843 | + | - | - | + | - | - | + | - | + | - | + | + | + | - | + | + | + | + | + | - | + |
API biotype100
@ref | GLU | FRU | GAL | TRE | MNE | SBE | MEL | SAC | RAF | MTE | MAL | LAC | LTE | MbGa | MaGa | CEL | GEN | MbGu | ESC | RIB | ARA | XYL | PLE | RHA | FUC | MLZ | DARL | LARL | XLT | DUL | TAG | GLY | INO | MAN | MTL | TUR | SOR | ADO | HBG | LYX | ERY | MDG | 3MDG | SAT | MUC | LTAT | DTAT | MTAT | DMLT | LMLT | CATE | TATE | TTE | CIT | GRT | GAT | 2KG | 5KG | TRY | NAG | GNT | PAC | PAT | pOBE | QAT | GTE | mOBE | BAT | PPAT | CMT | TGE | BET | PCE | ABT | HIN | LAT | CAP | CYT | HIS | SUC | FUM | GRE | GYT | AVT | ETN | TTN | GLN | ITA | 3OBU | APT | GTT | PRO | DALA | LALA | SER | MNT | PROP | TYR | 2KT |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
121968 | + | + | + | + | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - | + | - | - | - | + | - | - | + | + | - | - | - | + | + | + | - | - | - | + | - | - | + | - | - | - | + | + | - | - | + | + | + | - | - | + | - | - | + | - | + | + | + | - | + | + | + | - | - | + | - | - | - | + | + | + | - | + | + | + | + | + | + | + | + | + | + | - | + | - | + | + | + | + | + | + | + | + | + | + | + |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | continent | country | origin.country |
---|---|---|---|---|---|
5843 | cyanobacterial mat samples | McMurdo | Australia and Oceania | ||
59225 | Cyanobacterial mat sample | Australia and Oceania | Antarctica | ATA | |
121968 | Cyanobacterial materials | Antarctica | Antarctica | ATA |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Microbial community | #Microbial mat |
#Host | #Microbial | #Bacteria |
taxonmaps
- @ref: 69479
- File name: preview.99_199.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_23;96_61;97_89;98_98;99_199&stattab=map
- Last taxonomy: Pseudomonas
- 16S sequence: AJ537603
- Sequence Identity:
- Total samples: 8172
- soil counts: 874
- aquatic counts: 1631
- animal counts: 3216
- plant counts: 2451
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
5843 | 1 | Risk group (German classification) |
121968 | 1 | Risk group (French classification) |
Sequence information
16S sequences
- @ref: 5843
- description: Pseudomonas proteolytica partial 16S rRNA gene, strain CMS 64T
- accession: AJ537603
- length: 1512
- database: ena
- NCBI tax ID: 219574
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Pseudomonas proteolytica DSM 15321 | GCA_007858275 | contig | ncbi | 219574 |
66792 | Pseudomonas proteolytica strain DSM 15321 | 219574.6 | wgs | patric | 219574 |
GC content
@ref | GC-content |
---|---|
5843 | 57 |
30011 | 58.3 |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
motile | yes | 89.422 | yes |
gram-positive | no | 98.182 | yes |
anaerobic | no | 98.306 | yes |
aerobic | yes | 90.239 | yes |
halophile | no | 92.399 | no |
spore-forming | no | 95.095 | no |
glucose-util | yes | 93.979 | yes |
flagellated | yes | 85.736 | no |
thermophile | no | 99.924 | yes |
glucose-ferment | no | 89.073 | no |
External links
@ref: 5843
culture collection no.: DSM 15321, CCM 7690, CCUG 51515, CMS 64, CIP 108464, MTCC 4994
straininfo link
- @ref: 82305
- straininfo: 132026
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 15143013 | Psychrophilic pseudomonads from Antarctica: Pseudomonas antarctica sp. nov., Pseudomonas meridiana sp. nov. and Pseudomonas proteolytica sp. nov. | Reddy GSN, Matsumoto GI, Schumann P, Stackebrandt E, Shivaji S | Int J Syst Evol Microbiol | 10.1099/ijs.0.02827-0 | 2004 | Antarctic Regions, Carbon/metabolism, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fresh Water/microbiology, Molecular Sequence Data, Phenotype, Phylogeny, Pseudomonas/*classification/genetics/*isolation & purification/metabolism, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Species Specificity | Metabolism |
Phylogeny | 27259517 | Possible misidentification of species in the Pseudomonas fluorescens lineage as Burkholderia pseudomallei and Francisella tularensis, and emended descriptions of Pseudomonas brenneri,Pseudomonas gessardii and Pseudomonas proteolytica. | van den Beld MJC, Reinders E, Notermans DW, Reubsaet FAG | Int J Syst Evol Microbiol | 10.1099/ijsem.0.001206 | 2016 | Bacterial Typing Techniques, Burkholderia pseudomallei/*classification/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Francisella tularensis/*classification/isolation & purification, Netherlands, *Phylogeny, Pseudomonas/*classification/isolation & purification, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Species Specificity, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, *Water Microbiology, Water Supply | Enzymology |
Reference
@id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
---|---|---|---|---|---|---|
5843 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15321) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-15321 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
30011 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 26375 | 28776041 | |
42006 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/6089 | ||||
59225 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 51515) | https://www.ccug.se/strain?id=51515 | |||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
68369 | Automatically annotated from API 20NE | |||||
68382 | Automatically annotated from API zym | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
82305 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID132026.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
121968 | Curators of the CIP | Collection of Institut Pasteur (CIP 108464) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108464 |