Strain identifier
BacDive ID: 130876
Type strain: 
Species: Bacillus subtilis
Strain Designation: Amp-T18
Strain history: <- T. Clavel, TU Munich, Freising-Weihenstephan, Germany; Amp-T18
NCBI tax ID(s): 1423 (species)
version 8.1 (current version)
General
@ref: 22185
BacDive-ID: 130876
DSM-Number: 28592
keywords: genome sequence, 16S sequence, Bacteria, mesophilic, human pathogen
description: Bacillus subtilis Amp-T18 is a mesophilic human pathogen that was isolated from caecal content; wildtype C57BL/6 mouse treated with ampicillin.
NCBI tax id
- NCBI tax id: 1423
- Matching level: species
strain history
- @ref: 22185
- history: <- T. Clavel, TU Munich, Freising-Weihenstephan, Germany; Amp-T18
doi: 10.13145/bacdive130876.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Caryophanales
- family: Bacillaceae
- genus: Bacillus
- species: Bacillus subtilis
- full scientific name: Bacillus subtilis (Ehrenberg 1835) Cohn 1872 (Approved Lists 1980)
synonyms
- @ref: 20215
- synonym: Vibrio subtilis
@ref: 22185
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Bacillales
family: Bacillaceae
genus: Bacillus
species: Bacillus subtilis
full scientific name: Bacillus subtilis (Ehrenberg 1835) Cohn 1872
strain designation: Amp-T18
type strain: no
Morphology
colony morphology
- @ref: 22185
- incubation period: 2-3 days
Culture and growth conditions
culture medium
- @ref: 22185
- name: LB (Luria-Bertani) MEDIUM (DSMZ Medium 381)
- growth: yes
- link: https://mediadive.dsmz.de/medium/381
- composition: Name: LB (Luria-Bertani) MEDIUM (DSMZ Medium 381) Composition: Agar 20.0 g/l NaCl 10.0 g/l Tryptone 10.0 g/l Yeast extract 5.0 g/l Distilled water
culture temp
- @ref: 22185
- growth: positive
- type: growth
- temperature: 37
- range: mesophilic
Isolation, sampling and environmental information
isolation
- @ref: 22185
- sample type: caecal content; wildtype C57BL/6 mouse treated with ampicillin
- geographic location: Freising
- country: Germany
- origin.country: DEU
- continent: Europe
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Infection | #Patient | #Antibiotic treatment |
| #Host | #Mammals | #Muridae (Mouse/Rat) |
| #Host Body-Site | #Gastrointestinal tract | #Large intestine |
| #Host Body Product | #Gastrointestinal tract | #Caecal content |
taxonmaps
- @ref: 69479
- File name: preview.99_21.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_17;97_17;98_20;99_21&stattab=map
- Last taxonomy: Bacillus
- 16S sequence: KR364738
- Sequence Identity:
- Total samples: 18201
- soil counts: 3768
- aquatic counts: 2854
- animal counts: 9674
- plant counts: 1905
Safety information
risk assessment
- @ref: 22185
- pathogenicity human: yes, in single cases
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 22185
- description: Bacillus sp. Amp-T18 16S ribosomal RNA gene, partial sequence
- accession: KR364738
- length: 1451
- database: ena
- NCBI tax ID: 1796611
Genome sequences
- @ref: 66792
- description: Bacillus subtilis DSM 28592
- accession: GCA_024623085
- assembly level: contig
- database: ncbi
- NCBI tax ID: 1423
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| flagellated | yes | 90.082 | no |
| gram-positive | yes | 91.613 | no |
| anaerobic | no | 99.171 | no |
| aerobic | yes | 88.612 | no |
| halophile | yes | 83.108 | no |
| spore-forming | yes | 97.135 | no |
| glucose-ferment | no | 91.396 | no |
| thermophile | no | 97.105 | no |
| glucose-util | yes | 89.299 | no |
| motile | yes | 89.846 | no |
External links
@ref: 22185
culture collection no.: DSM 28592
straininfo link
- @ref: 89359
- straininfo: 403373
Reference
| @id | authors | title | doi/url | catalogue |
|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
| 22185 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-28592 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 28592) | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | |
| 89359 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID403373.1 |