Strain identifier
BacDive ID: 130854
Type strain:
Species: Acinetobacter lwoffii
Strain Designation: 2A
Strain history: <- J. Sowada, Bundesinstitut für Risikobewertung; 2A
NCBI tax ID(s): 28090 (species)
General
@ref: 22163
BacDive-ID: 130854
DSM-Number: 101862
keywords: genome sequence, 16S sequence, Bacteria, mesophilic
description: Acinetobacter lwoffii 2A is a mesophilic bacterium that was isolated from human volar forearm.
NCBI tax id
- NCBI tax id: 28090
- Matching level: species
strain history
- @ref: 22163
- history: <- J. Sowada, Bundesinstitut für Risikobewertung; 2A
doi: 10.13145/bacdive130854.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Pseudomonadales
- family: Moraxellaceae
- genus: Acinetobacter
- species: Acinetobacter lwoffii
- full scientific name: Acinetobacter lwoffii (Audureau 1940) Brisou and Prévot 1954 (Approved Lists 1980)
synonyms
- @ref: 20215
- synonym: Moraxella lwoffi
@ref: 22163
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Pseudomonadales
family: Moraxellaceae
genus: Acinetobacter
species: Acinetobacter lwoffii
full scientific name: Acinetobacter lwoffii (Audureau 1940) Brisou and Prévot 1954 emend. Nemec et al. 2019
strain designation: 2A
type strain: no
Morphology
cell morphology
@ref | motility | confidence | gram stain |
---|---|---|---|
69480 | no | 95.209 | |
69480 | 99.98 | negative |
colony morphology
- @ref: 22163
- incubation period: 1-2 days
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
22163 | NUTRIENT AGAR (DSMZ Medium 1) | yes | https://mediadive.dsmz.de/medium/1 | Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water |
22163 | COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) | yes | https://mediadive.dsmz.de/medium/693 | Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base |
22163 | TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) | yes | https://mediadive.dsmz.de/medium/92 | Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water |
culture temp
- @ref: 22163
- growth: positive
- type: growth
- temperature: 30
- range: mesophilic
Physiology and metabolism
spore formation
@ref | spore formation | confidence |
---|---|---|
69481 | no | 100 |
69480 | no | 99.993 |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
68369 | 25115 | malate | - | assimilation |
68369 | 24265 | gluconate | - | assimilation |
68369 | 17306 | maltose | - | assimilation |
68369 | 59640 | N-acetylglucosamine | - | assimilation |
68369 | 16899 | D-mannitol | - | assimilation |
68369 | 16024 | D-mannose | - | assimilation |
68369 | 30849 | L-arabinose | - | assimilation |
68369 | 17634 | D-glucose | - | assimilation |
68369 | 5291 | gelatin | - | hydrolysis |
68369 | 4853 | esculin | - | hydrolysis |
68369 | 16199 | urea | - | hydrolysis |
68369 | 29016 | arginine | - | hydrolysis |
68369 | 17634 | D-glucose | - | fermentation |
68369 | 27897 | tryptophan | - | energy source |
68369 | 17632 | nitrate | - | reduction |
metabolite production
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
@ref | value | activity | ec |
---|---|---|---|
22163 | catalase | + | 1.11.1.6 |
22163 | cytochrome-c oxidase | - | 1.9.3.1 |
68369 | gelatinase | - | |
68369 | beta-glucosidase | - | 3.2.1.21 |
68369 | urease | - | 3.5.1.5 |
68369 | arginine dihydrolase | - | 3.5.3.6 |
API 20NE
@ref | NO3 | TRP | GLU_ Ferm | ADH Arg | URE | ESC | GEL | PNPG | GLU_ Assim | ARA | MNE | MAN | NAG | MAL | GNT | CAP | ADI | MLT | CIT | PAC | OX |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
22163 | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | + | - | - | + | +/- |
22163 | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | + | - | - | - | + | - |
Isolation, sampling and environmental information
isolation
- @ref: 22163
- sample type: human volar forearm
- geographic location: Bundesinstitut für Risikobewertung, Berlin
- country: Germany
- origin.country: DEU
- continent: Europe
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Host | #Human | |
#Host Body-Site | #Limb | #Arm |
taxonmaps
- @ref: 69479
- File name: preview.99_43.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_8;96_11;97_38;98_41;99_43&stattab=map
- Last taxonomy: Acinetobacter
- 16S sequence: KF993657
- Sequence Identity:
- Total samples: 10947
- soil counts: 822
- aquatic counts: 2946
- animal counts: 6831
- plant counts: 348
Safety information
risk assessment
- @ref: 22163
- biosafety level: 2
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 22163
- description: Acinetobacter lwoffii strain 2A 16S ribosomal RNA gene, partial sequence
- accession: KF993657
- length: 1396
- database: ena
- NCBI tax ID: 28090
Genome sequences
- @ref: 66792
- description: Acinetobacter lwoffii strain FDAARGOS 1394
- accession: 28090.134
- assembly level: complete
- database: patric
- NCBI tax ID: 28090
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 100 | no |
motile | no | 92.049 | no |
gram-positive | no | 97.655 | no |
anaerobic | no | 98.691 | no |
halophile | no | 87.169 | no |
spore-forming | no | 96.877 | no |
glucose-util | no | 86.325 | no |
flagellated | no | 95.618 | no |
aerobic | yes | 93.279 | no |
thermophile | no | 99.86 | no |
glucose-ferment | no | 92.348 | yes |
External links
@ref: 22163
culture collection no.: DSM 101862
straininfo link
- @ref: 89338
- straininfo: 398563
Reference
@id | authors | title | doi/url | catalogue |
---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
22163 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-101862 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 101862) | |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
68369 | Automatically annotated from API 20NE | |||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | |
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | Predictions based on the model GenomeNet Sporulation v. 1 | 10.21203/rs.3.rs-2527258/v1 | |
89338 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID398563.1 |