Strain identifier

BacDive ID: 130854

Type strain: No

Species: Acinetobacter lwoffii

Strain Designation: 2A

Strain history: <- J. Sowada, Bundesinstitut für Risikobewertung; 2A

NCBI tax ID(s): 28090 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 22163

BacDive-ID: 130854

DSM-Number: 101862

keywords: genome sequence, 16S sequence, Bacteria, mesophilic

description: Acinetobacter lwoffii 2A is a mesophilic bacterium that was isolated from human volar forearm.

NCBI tax id

  • NCBI tax id: 28090
  • Matching level: species

strain history

  • @ref: 22163
  • history: <- J. Sowada, Bundesinstitut für Risikobewertung; 2A

doi: 10.13145/bacdive130854.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Moraxellaceae
  • genus: Acinetobacter
  • species: Acinetobacter lwoffii
  • full scientific name: Acinetobacter lwoffii (Audureau 1940) Brisou and Prévot 1954 (Approved Lists 1980)
  • synonyms

    • @ref: 20215
    • synonym: Moraxella lwoffi

@ref: 22163

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Moraxellaceae

genus: Acinetobacter

species: Acinetobacter lwoffii

full scientific name: Acinetobacter lwoffii (Audureau 1940) Brisou and Prévot 1954 emend. Nemec et al. 2019

strain designation: 2A

type strain: no

Morphology

cell morphology

@refmotilityconfidencegram stain
69480no95.209
6948099.98negative

colony morphology

  • @ref: 22163
  • incubation period: 1-2 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
22163NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
22163COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
22163TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water

culture temp

  • @ref: 22163
  • growth: positive
  • type: growth
  • temperature: 30
  • range: mesophilic

Physiology and metabolism

spore formation

@refspore formationconfidence
69481no100
69480no99.993

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate-assimilation
6836924265gluconate-assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose-assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
22163catalase+1.11.1.6
22163cytochrome-c oxidase-1.9.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
22163----------------+--++/-
22163---------------+---+-

Isolation, sampling and environmental information

isolation

  • @ref: 22163
  • sample type: human volar forearm
  • geographic location: Bundesinstitut für Risikobewertung, Berlin
  • country: Germany
  • origin.country: DEU
  • continent: Europe

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body-Site#Limb#Arm

taxonmaps

  • @ref: 69479
  • File name: preview.99_43.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_8;96_11;97_38;98_41;99_43&stattab=map
  • Last taxonomy: Acinetobacter
  • 16S sequence: KF993657
  • Sequence Identity:
  • Total samples: 10947
  • soil counts: 822
  • aquatic counts: 2946
  • animal counts: 6831
  • plant counts: 348

Safety information

risk assessment

  • @ref: 22163
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 22163
  • description: Acinetobacter lwoffii strain 2A 16S ribosomal RNA gene, partial sequence
  • accession: KF993657
  • length: 1396
  • database: ena
  • NCBI tax ID: 28090

Genome sequences

  • @ref: 66792
  • description: Acinetobacter lwoffii strain FDAARGOS 1394
  • accession: 28090.134
  • assembly level: complete
  • database: patric
  • NCBI tax ID: 28090

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno92.049no
gram-positiveno97.655no
anaerobicno98.691no
halophileno87.169no
spore-formingno96.877no
glucose-utilno86.325no
flagellatedno95.618no
aerobicyes93.279no
thermophileno99.86no
glucose-fermentno92.348yes

External links

@ref: 22163

culture collection no.: DSM 101862

straininfo link

  • @ref: 89338
  • straininfo: 398563

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
22163Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-101862Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 101862)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68369Automatically annotated from API 20NE
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
89338Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID398563.1