Strain identifier

BacDive ID: 13081

Type strain: No

Species: Pseudomonas nitroreducens

Strain Designation: IpA-1

Strain history: CIP <- 2000, W.W. Mohn, British Columbia Univ., Vancouver, Canada, Pseudomonas multiresinivorans: strain IpA-1 <- A.E. Wilson

NCBI tax ID(s): 95301 (species)

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General

@ref: 6969

BacDive-ID: 13081

DSM-Number: 17553

keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Pseudomonas nitroreducens IpA-1 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from sequencing batch reactor.

NCBI tax id

  • NCBI tax id: 95301
  • Matching level: species

strain history

@refhistory
6969<- W. W. Mohn <- A. E. Wilson; IpA-1
67770IAM 15165 <-- CIP 106706 <-- W. W. Mohn IpA-1 <-- A. E. Wilson.
121315CIP <- 2000, W.W. Mohn, British Columbia Univ., Vancouver, Canada, Pseudomonas multiresinivorans: strain IpA-1 <- A.E. Wilson

doi: 10.13145/bacdive13081.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas nitroreducens
  • full scientific name: Pseudomonas nitroreducens Iizuka and Komagata 1964 (Approved Lists 1980)

  • synonyms

    @refsynonym
    20215Pseudomonas nitritireducens
    20215Pseudomonas multiresinivorans

@ref: 6969

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas nitroreducens

full scientific name: Pseudomonas nitroreducens Iizuka and Komagata 1964 emend. Lang et al. 2007

strain designation: IpA-1

type strain: no

Morphology

cell morphology

  • @ref: 121315
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

pigmentation

  • @ref: 121315
  • production: no
  • name: Pyocyanin

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6969TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
35982MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
121315CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
6969positivegrowth30mesophilic
35982positivegrowth30mesophilic
59270positivegrowth30-37mesophilic
67770positivegrowth30mesophilic
121315positivegrowth5-37
121315nogrowth41thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
59270aerobe
121315obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
121315NaClpositivegrowth0-6 %
121315NaClnogrowth8 %
121315NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate+assimilation
6836927689decanoate+assimilation
6836924265gluconate+assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose+assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine+hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate+reduction
12131516947citrate-carbon source
1213154853esculin-hydrolysis
12131517632nitrate+reduction
12131516301nitrite+reduction
12131517632nitrate+respiration

antibiotic resistance

  • @ref: 121315
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleno
12131535581indoleno

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369cytochrome oxidase+1.9.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase+3.5.3.6
121315oxidase+
121315beta-galactosidase-3.2.1.23
121315alcohol dehydrogenase+1.1.1.1
121315gelatinase-
121315amylase-
121315DNase-
121315caseinase-3.4.21.50
121315catalase+1.11.1.6
121315tween esterase-
121315lecithinase-
121315lipase-
121315lysine decarboxylase-4.1.1.18
121315ornithine decarboxylase-4.1.1.17
121315protease-
121315tryptophan deaminase-
121315urease-3.5.1.5
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
59270-+++-+----++--------
121315--++-+----++--------

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
59270+--+----+-----+++++++
6969+--+----+-----+++++++

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
121315++-----------------------------+---------------+++++++------+++++--+---+++++++-+++-+++-+++++++-++--

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling date
6969sequencing batch reactorVancouverCanadaCANNorth America
59270Sequencing batch bioreactorVancouverCanadaCANNorth America1995
121315Sequencing batch bioreactorVancouverCanadaCANNorth America

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Bioreactor

taxonmaps

  • @ref: 69479
  • File name: preview.99_295.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_23;96_203;97_221;98_250;99_295&stattab=map
  • Last taxonomy: Pseudomonas
  • 16S sequence: X96787
  • Sequence Identity:
  • Total samples: 5736
  • soil counts: 862
  • aquatic counts: 2237
  • animal counts: 2039
  • plant counts: 598

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
69691Risk group (German classification)
1213151Risk group (French classification)

Sequence information

16S sequences

  • @ref: 6969
  • description: Pseudomonas multiresinivorans 16S rRNA gene, type strain ATCC 700690T
  • accession: X96787
  • length: 1492
  • database: ena
  • NCBI tax ID: 95301

External links

@ref: 6969

culture collection no.: CCUG 51655, DSM 17553, ATCC 700690, CIP 106706, JCM 21620, IAM 15165, LMG 20221, NBRC 103160

straininfo link

  • @ref: 82291
  • straininfo: 14974

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny17329787Pseudomonas knackmussii sp. nov.Stolz A, Busse HJ, Kampfer PInt J Syst Evol Microbiol10.1099/ijs.0.64761-02007DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/analysis, Molecular Sequence Data, Pseudomonas/chemistry/*classification/genetics/physiology, RNA, Ribosomal, 16S/geneticsGenetics
Phylogeny18523175Pseudomonas panipatensis sp. nov., isolated from an oil-contaminated site.Gupta SK, Kumari R, Prakash O, Lal RInt J Syst Evol Microbiol10.1099/ijs.0.65401-02008Bacterial Typing Techniques, Base Sequence, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Fatty Acids/analysis, Genes, rRNA, India, Molecular Sequence Data, Nucleic Acid Hybridization, *Petroleum, Phenotype, Phylogeny, Pseudomonas/*classification/genetics/*isolation & purification/physiology, RNA, Ribosomal, 16S/chemistry/genetics, Sequence Analysis, DNA, *Soil Microbiology, *Soil Pollutants, Species SpecificityGenetics

Reference

@idauthorscataloguedoi/urltitle
6969Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17553)https://www.dsmz.de/collection/catalogue/details/culture/DSM-17553
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
35982Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/18865
59270Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 51655)https://www.ccug.se/strain?id=51655
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
82291Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID14974.1StrainInfo: A central database for resolving microbial strain identifiers
121315Curators of the CIPCollection of Institut Pasteur (CIP 106706)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106706