Strain identifier

BacDive ID: 130796

Type strain: Yes

Species: Prauserella soli

Strain Designation: 12-833, Ac+54, Actinomycetes 54

Strain history: <- A. Al-Mutairi, Kuwait Univ. Kuwait; Ac+54

NCBI tax ID(s): 1356851 (species)

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General

@ref: 21855

BacDive-ID: 130796

DSM-Number: 45819

keywords: 16S sequence, Bacteria, mesophilic

description: Prauserella soli 12-833 is a mesophilic bacterium that was isolated from crude oil-contaminated soil.

NCBI tax id

  • NCBI tax id: 1356851
  • Matching level: species

strain history

  • @ref: 21855
  • history: <- A. Al-Mutairi, Kuwait Univ. Kuwait; Ac+54

doi: 10.13145/bacdive130796.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Pseudonocardiales
  • family: Pseudonocardiaceae
  • genus: Prauserella
  • species: Prauserella soli
  • full scientific name: Prauserella soli Almutairi 2015

@ref: 21855

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Pseudonocardiaceae

genus: Prauserella

species: Prauserella soli

full scientific name: Prauserella soli Almutairi 2015

strain designation: 12-833, Ac+54, Actinomycetes 54

type strain: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
21855GPHF-MEDIUM (DSMZ Medium 553)yeshttps://mediadive.dsmz.de/medium/553Name: GPHF-MEDIUM (DSMZ Medium 553) Composition: Agar 20.0 g/l Glucose 10.0 g/l Beef extract 5.0 g/l Yeast extract 5.0 g/l Casein peptone 5.0 g/l CaCl2 x 2 H2O 0.74 g/l Distilled water
21855GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water

culture temp

  • @ref: 21855
  • growth: positive
  • type: growth
  • temperature: 28
  • range: mesophilic

Physiology and metabolism

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836927689decanoate-assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
683695291gelatin-hydrolysis
683694853esculin+hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369gelatinase-
68369beta-glucosidase+3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6
68382alkaline phosphatase-3.1.3.1
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase-3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
21855--+/-+-+-----------+--

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPAC
21855-----+--+/------+/--+/-+/-+/--

Isolation, sampling and environmental information

isolation

  • @ref: 21855
  • sample type: crude oil-contaminated soil
  • country: Kuwait
  • origin.country: KWT
  • continent: Asia

isolation source categories

Cat1Cat2Cat3
#Engineered#Contamination#Oil (Fuel)
#Engineered#Industrial#Oil reservoir
#Environmental#Terrestrial#Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_117568.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_7302;97_29092;98_37117;99_117568&stattab=map
  • Last taxonomy: Prauserella soli
  • 16S sequence: KC514355
  • Sequence Identity:
  • Total samples: 68
  • soil counts: 21
  • aquatic counts: 6
  • animal counts: 40
  • plant counts: 1

Safety information

risk assessment

  • @ref: 21855
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 21855
  • description: Prauserella soli strain 12-833 16S ribosomal RNA gene, partial sequence
  • accession: KC514355
  • length: 1525
  • database: ena
  • NCBI tax ID: 1356851

GC content

  • @ref: 21855
  • GC-content: 71.1
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 21855

culture collection no.: DSM 45819, KCTC 39766, LMG 28346

straininfo link

  • @ref: 89286
  • straininfo: 398681

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny26070693Prauserella soli sp. nov., isolated from crude oil-contaminated soil.Almutairi AInt J Syst Evol Microbiol10.1099/ijs.0.0003782015*Actinomycetales/classification, Base Composition, DNA, Bacterial/genetics, Environmental Pollution, Fatty Acids/chemistry, Molecular Sequence Data, Petroleum, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Soil MicrobiologyGenetics
Phylogeny31671051Prauserella flavalba sp. nov., a novel species of the genus Prauserella, isolated from alkaline soil.Li Q, Ruckert C, Li G, Huang P, Schneider O, Kalinowski J, Jiang Y, Zotchev SB, Jiang CInt J Syst Evol Microbiol10.1099/ijsem.0.0037682020Actinobacteria/*classification/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistryTranscriptome

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
21855Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-45819Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 45819)
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
89286Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID398681.1