Strain identifier
BacDive ID: 130796
Type strain:
Species: Prauserella soli
Strain Designation: 12-833, Ac+54, Actinomycetes 54
Strain history: <- A. Al-Mutairi, Kuwait Univ. Kuwait; Ac+54
NCBI tax ID(s): 1356851 (species)
General
@ref: 21855
BacDive-ID: 130796
DSM-Number: 45819
keywords: 16S sequence, Bacteria, mesophilic
description: Prauserella soli 12-833 is a mesophilic bacterium that was isolated from crude oil-contaminated soil.
NCBI tax id
- NCBI tax id: 1356851
- Matching level: species
strain history
- @ref: 21855
- history: <- A. Al-Mutairi, Kuwait Univ. Kuwait; Ac+54
doi: 10.13145/bacdive130796.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Pseudonocardiales
- family: Pseudonocardiaceae
- genus: Prauserella
- species: Prauserella soli
- full scientific name: Prauserella soli Almutairi 2015
@ref: 21855
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Pseudonocardiaceae
genus: Prauserella
species: Prauserella soli
full scientific name: Prauserella soli Almutairi 2015
strain designation: 12-833, Ac+54, Actinomycetes 54
type strain: yes
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
21855 | GPHF-MEDIUM (DSMZ Medium 553) | yes | https://mediadive.dsmz.de/medium/553 | Name: GPHF-MEDIUM (DSMZ Medium 553) Composition: Agar 20.0 g/l Glucose 10.0 g/l Beef extract 5.0 g/l Yeast extract 5.0 g/l Casein peptone 5.0 g/l CaCl2 x 2 H2O 0.74 g/l Distilled water |
21855 | GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) | yes | https://mediadive.dsmz.de/medium/65 | Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water |
culture temp
- @ref: 21855
- growth: positive
- type: growth
- temperature: 28
- range: mesophilic
Physiology and metabolism
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
68369 | 27689 | decanoate | - | assimilation |
68369 | 17306 | maltose | - | assimilation |
68369 | 59640 | N-acetylglucosamine | - | assimilation |
68369 | 16899 | D-mannitol | - | assimilation |
68369 | 16024 | D-mannose | - | assimilation |
68369 | 30849 | L-arabinose | - | assimilation |
68369 | 5291 | gelatin | - | hydrolysis |
68369 | 4853 | esculin | + | hydrolysis |
68369 | 16199 | urea | - | hydrolysis |
68369 | 29016 | arginine | - | hydrolysis |
68369 | 17634 | D-glucose | - | fermentation |
68369 | 27897 | tryptophan | - | energy source |
68369 | 17632 | nitrate | - | reduction |
metabolite production
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
@ref | value | activity | ec |
---|---|---|---|
68369 | gelatinase | - | |
68369 | beta-glucosidase | + | 3.2.1.21 |
68369 | urease | - | 3.5.1.5 |
68369 | arginine dihydrolase | - | 3.5.3.6 |
68382 | alkaline phosphatase | - | 3.1.3.1 |
68382 | esterase lipase (C 8) | + | |
68382 | lipase (C 14) | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | valine arylamidase | - | |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | trypsin | - | 3.4.21.4 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | acid phosphatase | - | 3.1.3.2 |
68382 | naphthol-AS-BI-phosphohydrolase | - | |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | + | 3.2.1.52 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
21855 | - | - | +/- | + | - | + | - | - | - | - | - | - | - | - | - | - | - | + | - | - |
API 20NE
@ref | NO3 | TRP | GLU_ Ferm | ADH Arg | URE | ESC | GEL | PNPG | GLU_ Assim | ARA | MNE | MAN | NAG | MAL | GNT | CAP | ADI | MLT | CIT | PAC |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
21855 | - | - | - | - | - | + | - | - | +/- | - | - | - | - | - | +/- | - | +/- | +/- | +/- | - |
Isolation, sampling and environmental information
isolation
- @ref: 21855
- sample type: crude oil-contaminated soil
- country: Kuwait
- origin.country: KWT
- continent: Asia
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Engineered | #Contamination | #Oil (Fuel) |
#Engineered | #Industrial | #Oil reservoir |
#Environmental | #Terrestrial | #Soil |
taxonmaps
- @ref: 69479
- File name: preview.99_117568.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_7302;97_29092;98_37117;99_117568&stattab=map
- Last taxonomy: Prauserella soli
- 16S sequence: KC514355
- Sequence Identity:
- Total samples: 68
- soil counts: 21
- aquatic counts: 6
- animal counts: 40
- plant counts: 1
Safety information
risk assessment
- @ref: 21855
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 21855
- description: Prauserella soli strain 12-833 16S ribosomal RNA gene, partial sequence
- accession: KC514355
- length: 1525
- database: ena
- NCBI tax ID: 1356851
GC content
- @ref: 21855
- GC-content: 71.1
- method: high performance liquid chromatography (HPLC)
External links
@ref: 21855
culture collection no.: DSM 45819, KCTC 39766, LMG 28346
straininfo link
- @ref: 89286
- straininfo: 398681
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 26070693 | Prauserella soli sp. nov., isolated from crude oil-contaminated soil. | Almutairi A | Int J Syst Evol Microbiol | 10.1099/ijs.0.000378 | 2015 | *Actinomycetales/classification, Base Composition, DNA, Bacterial/genetics, Environmental Pollution, Fatty Acids/chemistry, Molecular Sequence Data, Petroleum, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Soil Microbiology | Genetics |
Phylogeny | 31671051 | Prauserella flavalba sp. nov., a novel species of the genus Prauserella, isolated from alkaline soil. | Li Q, Ruckert C, Li G, Huang P, Schneider O, Kalinowski J, Jiang Y, Zotchev SB, Jiang C | Int J Syst Evol Microbiol | 10.1099/ijsem.0.003768 | 2020 | Actinobacteria/*classification/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistry | Transcriptome |
Reference
@id | authors | title | doi/url | catalogue |
---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
21855 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-45819 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 45819) | |
68369 | Automatically annotated from API 20NE | |||
68382 | Automatically annotated from API zym | |||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | |
89286 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID398681.1 |