Strain identifier

BacDive ID: 130763

Type strain: Yes

Species: Vibrio cholerae

Strain Designation: strain 3, J4308

Strain history: <- CIP <- NCTC <- H.J. Bensted, Public Health Laboratory Services, Colindale, London, United Kingdom; strain 3

NCBI tax ID(s): 1408478 (strain), 666 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 21977

BacDive-ID: 130763

DSM-Number: 100200

keywords: antibiotic resistance, mesophilic, Bacteria, 16S sequence, genome sequence

description: Vibrio cholerae strain 3 is a mesophilic bacterium that has multiple antibiotic resistances.

NCBI tax id

NCBI tax idMatching level
666species
1408478strain

strain history: <- CIP <- NCTC <- H.J. Bensted, Public Health Laboratory Services, Colindale, London, United Kingdom; strain 3

doi: 10.13145/bacdive130763.20220920.7

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Vibrionales
  • family: Vibrionaceae
  • genus: Vibrio
  • species: Vibrio cholerae
  • full scientific name: Vibrio cholerae Pacini 1854 (Approved Lists 1980)
  • synonyms

    • @ref: 20215
    • synonym: Vibrio albensis

@ref: 21977

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Vibrionales, not validated

family: Vibrionaceae

genus: Vibrio

species: Vibrio cholerae

full scientific name: Vibrio cholerae Pacini 1854

strain designation: strain 3, J4308

type strain: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
21977COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://bacmedia.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
21977TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://bacmedia.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
39747MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)

culture temp

@refgrowthtypetemperaturerange
21977positivegrowth28mesophilic
39747positivegrowth30mesophilic

Physiology and metabolism

antibiogram

@refmediumincubation temperatureoxygen conditionPenicillin Goxacillinampicillinticarcillinmezlocillincefalotincefazolincefotaximeaztreonamimipenemtetracyclinechloramphenicolgentamycinamikacinvancomycinerythromycinlincomycinofloxacinnorfloxacincolistinpipemidic acidnitrofurantoinbacitracinpolymyxin bkanamycinneomycindoxycyclineceftriaxoneclindamycinfosfomycinmoxifloxacinlinezolidnystatinquinupristin/dalfopristinteicoplaninpiperacillin/tazobactam
21977Mueller-Hinton Agar30aerob160203234-36302644342834-363824240228424612-143628014-1624-2620-22324210104028014032
21977Mueller-Hinton Agar30aerob160203032-343024423428363622220200404014343001624203438-401012-1436-383012-14161034
21977Mueller-Hinton Agar30aerob160203032-343024423428363622220200404014343001624203438-401012-1436-383012-14161034

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate-assimilation
6836927689decanoate-assimilation
6836924265gluconate+assimilation
6836917306maltose+assimilation
6836959640N-acetylglucosamine+assimilation
6836916899D-mannitol+assimilation
6836916024D-mannose+assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose+assimilation
683695291gelatin+hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose+fermentation
6836927897tryptophan+energy source
6836917632nitrate+reduction
6836830849L-arabinose-fermentation
6836827613amygdalin-fermentation
6836828053melibiose-fermentation
6836817992sucrose+fermentation
6836862345L-rhamnose-fermentation
6836830911sorbitol-fermentation
6836817268myo-inositol-fermentation
6836816899D-mannitol+fermentation
6836817634D-glucose+fermentation
683685291gelatin+hydrolysis
6836827897tryptophan+energy source
6836816199urea-hydrolysis
6836816947citrate+assimilation
6836818257ornithine+degradation
6836825094lysine+degradation
6836829016arginine-hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleyes
6836815688acetoinyes
6836835581indoleyes
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-test
6836935581indole+
6836815688acetoin+
6836835581indole+

enzymes

@refvalueactivityec
68369cytochrome oxidase+1.9.3.1
68369gelatinase+
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6
68368cytochrome oxidase+1.9.3.1
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase+4.1.1.17
68368lysine decarboxylase+4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase+3.2.1.23

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOX
21977+-+++---+++++---+---+
21977+-+++---+++++---+---+
21977+-+++---+++++---+---+

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
21977+++---+++-+++++--++-+

Isolation, sampling and environmental information

isolation

  • @ref: 21977
  • geographic location: unknown?

Safety information

risk assessment

  • @ref: 21977
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 21977
  • description: V.cholerae (CECT 514 T) 16S rRNA gene
  • accession: X76337
  • length: 1538
  • database: ena
  • NCBI tax ID: 666

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Vibrio cholerae ATCC 14035 ATCC 14035GCA_000621645scaffoldncbi1408478
66792Vibrio cholerae CECT 514GCA_013155105completencbi666
66792Vibrio cholerae ATCC 140351408478.3wgspatric1408478
66792Vibrio cholerae strain CECT 514666.5088completepatric666
66792Vibrio cholerae ATCC 140352556921658draftimg1408478

External links

@ref: 21977

culture collection no.: CCUG 9118 A, DSM 100200, ATCC 14035, CCUG 9118, CIP 62.13, NCTC 8021, CECT 514

straininfo link

@refpassport
20218http://www.straininfo.net/strains/97285
20218http://www.straininfo.net/strains/156129
20218http://www.straininfo.net/strains/234997
20218http://www.straininfo.net/strains/97286

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny7474354[Causative agent of the so-called "light disease of shrimps" is luminescent Vibrio cholerae non-O1].Shimada T, Arakawa E, Itoh K, Kosako Y, Okitsu T, Yamai S, Nishino M, Nakajima TNihon Saikingaku Zasshi10.3412/jsb.50.8631995Animals, Decapoda/*microbiology, Luminescent Measurements, Serotyping, Vibrio cholerae/*classification/isolation & purification/metabolismEnzymology
Metabolism7567791Characterization of the melanogenic system in Vibrio cholerae, ATCC 14035.Ruzafa C, Sanchez-Amat A, Solano FPigment Cell Res10.1111/j.1600-0749.1995.tb00656.x1995Chromatography, High Pressure Liquid, Culture Media, Homogentisic Acid/metabolism, Hydrogen-Ion Concentration, Kinetics, Melanins/*biosynthesis, Monophenol Monooxygenase/metabolism, Phenylpyruvic Acids/metabolism, Transaminases/metabolism, Tyrosine, Vibrio cholerae/*metabolismCultivation
Metabolism9734339Comparative tyrosine degradation in Vibrio cholerae strains. The strain ATCC 14035 as a prokaryotic melanogenic model of homogentisate-releasing cell.Sanchez-Amat A, Ruzafa C, Solano FComp Biochem Physiol B Biochem Mol Biol10.1016/s0305-0491(98)00028-51998*Dioxygenases, Enzyme Induction, Homogentisate 1,2-Dioxygenase, Homogentisic Acid/metabolism, Hydrolases/biosynthesis/metabolism, Kinetics, Models, Biological, Oxygenases/biosynthesis/metabolism, Serotyping, Tyrosine/*metabolism, Vibrio cholerae/*classification/growth & development/*metabolism, cis-trans-Isomerases/biosynthesis/metabolismEnzymology
Enzymology10049907Role of surface proteins in Vibrio cholerae attachment to chitin.Tarsi R, Pruzzo CAppl Environ Microbiol10.1128/AEM.65.3.1348-1351.19991999Acetylglucosamine/pharmacology, *Bacterial Adhesion/drug effects, Chitin/*physiology, Disaccharides/pharmacology, Electrophoresis, Polyacrylamide Gel, Membrane Proteins/*metabolism, Periodic Acid/pharmacology, Pronase/metabolism, Vibrio cholerae/*physiology, Wheat Germ Agglutinins/pharmacologyMetabolism
Pathogenicity10347079Response of pathogenic Vibrio species to high hydrostatic pressure.Berlin DL, Herson DS, Hicks DT, Hoover DGAppl Environ Microbiol10.1128/AEM.65.6.2776-2780.19991999Colony Count, Microbial, Disinfection/methods, Humans, *Hydrostatic Pressure, Vibrio/growth & development/isolation & purification/*physiology, Vibrio Infections/microbiologyEnzymology
Enzymology23508422Comparison of two recombinant systems for expression of cholera toxin B subunit from Vibrio cholerae.Boustanshenas M, Bakhshi B, Ghorbani M, Norouzian DIndian J Med Microbiol10.4103/0255-0857.1087052013Cholera Toxin/*biosynthesis/genetics, Cloning, Molecular, Escherichia coli/genetics, *Gene Expression, Genetic Vectors, Plasmids, Polymerase Chain Reaction, Recombinant Proteins/biosynthesis/genetics, Vibrio cholerae/genetics
24165439The hows and whys of constructing a native recombinant cholera vaccine.Boustanshenas M, Bakhshi BBioengineered10.4161/bioe.264202013Animals, Cholera Toxin/*immunology, Cholera Vaccines/*immunology, Female
Enzymology24842138A single point mutation within the coding sequence of cholera toxin B subunit will increase its expression yield.Bakhshi B, Boustanshenas M, Ghorbani MIran Biomed J10.6091/ibj.1165.20142014Amino Acid Sequence, Blotting, Western, Cholera Toxin/*genetics/*metabolism, Cloning, Molecular, DNA/metabolism, Electrophoresis, Agar Gel, Electrophoresis, Polyacrylamide Gel, Mutant Proteins/metabolism, Open Reading Frames/*genetics, Point Mutation/*genetics, Polymerase Chain Reaction, Recombinant Proteins/metabolismMetabolism
Phylogeny29665368Streptomyces thermoviolaceus SRC3 strain as a novel source of the antibiotic adjuvant streptazolin: A statistical approach toward the optimized production.Djinni I, Djoudi W, Souagui S, Rabia F, Rahmouni S, Mancini I, Kecha MJ Microbiol Methods10.1016/j.mimet.2018.04.0082018Algeria, Anti-Infective Agents/*isolation & purification/*metabolism, Bacteriological Techniques, Candida albicans/drug effects, Culture Media/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Geologic Sediments/microbiology, Methicillin-Resistant Staphylococcus aureus/drug effects, Piperidines/*isolation & purification/*metabolism, RNA, Ribosomal, 16S/genetics, Rivers/microbiology, Salmonella typhi/drug effects, Sequence Analysis, DNA, Streptomyces/classification/*growth & development/isolation & purification/*metabolism, Time Factors, Vibrio cholerae/drug effectsPathogenicity
30636952Growth Inhibitory Effect of Lactocare on Vibrio cholerae.Asadi M, Fazeli MR, Sabokbar AIran J Pathol2018
31143687Capsaicin inhibitory effects on Vibrio cholerae toxin genes expression.Erfanimanesh S, Eslami G, Taherpour A, Hashemi AAvicenna J Phytomed2019
32944045Genetic Diversity of ctxB Gene Among Classical O1 and El Tor Strains of Vibrio cholerae using High-Resolution Melting Curve Analysis.Mahboobi M, Mirnejad R, Sedighian H, Piranfar V, Imani Fooladi AAIran J Pathol10.30699/ijp.2020.127793.23932020
Pathogenicity33913531Vibrio cholerae toxin coregulated pilus provokes inflammatory responses in Coculture model of Caco-2 and peripheral blood mononuclear cells (PBMC) leading to increased colonization.Ghasemi M, Bakhshi B, Khashei R, Soudi S, Boustanshenas MMicrobiol Immunol10.1111/1348-0421.128892021Antigens, CD/immunology, Caco-2 Cells, Cell Adhesion Molecules/immunology, *Cholera, Cholera Toxin/*immunology, Coculture Techniques, Cytokines/immunology, Fimbriae Proteins/*immunology, Humans, Leukocytes, Mononuclear/*immunology/microbiology, *Vibrio cholerae
Metabolism35171023Pufferfish (Tetraodon cutcutia) Sampled from a Freshwater River Serves as an Intermediate Reservoir of a Sucrose Nonfermenting Variant of Vibrio cholerae PS-4.Das L, Deb S, Arakawa E, Yamasaki S, Das SKMicrobiol Spectr10.1128/spectrum.01221-212022Animals, Bacterial Proteins/genetics/metabolism, Cholera/microbiology, Disease Reservoirs/*microbiology, Endotoxins/metabolism, Fermentation, Fructose/metabolism, Genome, Bacterial, Glucose/metabolism, Humans, Phosphotransferases/genetics/metabolism, Rivers/microbiology, Skin/microbiology, Sucrose/*metabolism, Tetraodontiformes/*microbiology, Vibrio cholerae/genetics/isolation & purification/*metabolismPathogenicity
35368520Investigation of the Antibacterial and Antibiofilm Activity of Selenium Nanoparticles against Vibrio cholerae as a Potent Therapeutics.Bagheri-Josheghani S, Bakhshi BCan J Infect Dis Med Microbiol10.1155/2022/34322352022

Reference

@idauthorstitledoi/urljournalpubmedcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.StrainInfo introduces electronic passports for microorganisms.10.1016/j.syapm.2013.11.002Syst Appl Microbiol. 37: 42-50 201424321274
21977Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-100200Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 100200)
39747Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/9958
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68368Automatically annotated from API 20E
68369Automatically annotated from API 20NE