Strain identifier

BacDive ID: 13075

Type strain: Yes

Species: Pseudomonas thermotolerans

Strain Designation: CM3

Strain history: CIP <- 2002, C.M. Manaia, Porto, Portugal: strain CM3

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 5303

BacDive-ID: 13075

DSM-Number: 14292

keywords: genome sequence, 16S sequence, Bacteria, aerobe, thermophilic, motile

description: Pseudomonas thermotolerans CM3 is an aerobe, thermophilic, motile bacterium that was isolated from cooking water of cork transformation industry.

NCBI tax id

NCBI tax idMatching level
1123021strain
157784species

strain history

@refhistory
5303<- C. M. Manaia, Porto; CM3
407152002, C.M. Manaia, Porto, Portugal: strain CM3
123518CIP <- 2002, C.M. Manaia, Porto, Portugal: strain CM3

doi: 10.13145/bacdive13075.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas thermotolerans
  • full scientific name: Pseudomonas thermotolerans Manaia and Moore 2002

@ref: 5303

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas thermotolerans

full scientific name: Pseudomonas thermotolerans Manaia and Moore 2002

strain designation: CM3

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480yes97.975
69480100negative
123518yesnegativerod-shaped

pigmentation

  • @ref: 123518
  • production: no
  • name: Pyocyanin

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5303NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
5303MINERAL MEDIUM (BRUNNER) (DSMZ Medium 457)yeshttps://mediadive.dsmz.de/medium/457Name: MINERAL MEDIUM (BRUNNER) (DSMZ Medium 457) Composition: Na2HPO4 2.44 g/l KH2PO4 1.52 g/l (NH4)2SO4 0.5 g/l MgSO4 x 7 H2O 0.2 g/l CaCl2 x 2 H2O 0.05 g/l EDTA 0.005 g/l FeSO4 x 7 H2O 0.002 g/l H3BO3 0.0003 g/l CoCl2 x 6 H2O 0.0002 g/l ZnSO4 x 7 H2O 0.0001 g/l MnCl2 x 4 H2O 3e-05 g/l Na2MoO4 x 2 H2O 3e-05 g/l NiCl2 x 6 H2O 2e-05 g/l CuCl2 x 2 H2O 1e-05 g/l Distilled water
5303PSEUDOMONAS AGAR F (DSMZ Medium 907)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium907.pdf
40715MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
123518CIP Medium 368yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=368
123518CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
5303positivegrowth45thermophilic
40715positivegrowth50thermophilic
60265positivegrowth30-42
5303positivegrowth37mesophilic
123518positivegrowth25-55
123518nogrowth5psychrophilic
123518nogrowth10psychrophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
60265aerobe
123518obligate aerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.995

halophily

@refsaltgrowthtested relationconcentration
123518NaClpositivegrowth0-4 %
123518NaClnogrowth6 %
123518NaClnogrowth8 %
123518NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate-assimilation
6836927689decanoate+assimilation
6836924265gluconate-assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose-assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction
12351816947citrate-carbon source
1235184853esculin-hydrolysis
12351817632nitrate-reduction
12351816301nitrite-reduction
12351817632nitrate-respiration

antibiotic resistance

  • @ref: 123518
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleno
12351835581indoleno

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369cytochrome oxidase+1.9.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6
123518oxidase+
123518beta-galactosidase-3.2.1.23
123518alcohol dehydrogenase-1.1.1.1
123518gelatinase-
123518amylase-
123518DNase-
123518caseinase-3.4.21.50
123518catalase+1.11.1.6
123518tween esterase-
123518lecithinase-
123518lipase-
123518lysine decarboxylase-4.1.1.18
123518ornithine decarboxylase-4.1.1.17
123518protease+
123518tryptophan deaminase-
123518urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123518-+++-++---++--------

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
5303---------------+-+--+
5303---------------+-+--+

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
123518------------------------------------------------++------------+++-+----+++-+++-+++-+----+++++++-+++

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
5303cooking water of cork transformation industryOportoPortugalPRTEurope
60265Cooking water of cork transformation industryPortugalPRTEurope
123518Environment, Water used to cookPortugalPRTEurope1998

isolation source categories

Cat1Cat2Cat3
#Engineered#Industrial#Industrial production
#Engineered#Waste#Industrial wastewater
#Host Body-Site#Plant#Bark

taxonmaps

  • @ref: 69479
  • File name: preview.99_5419.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_23;96_646;97_3219;98_4043;99_5419&stattab=map
  • Last taxonomy: Azotobacter
  • 16S sequence: AJ311980
  • Sequence Identity:
  • Total samples: 2713
  • soil counts: 333
  • aquatic counts: 943
  • animal counts: 1281
  • plant counts: 156

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
53031Risk group (German classification)
1235181Risk group (French classification)

Sequence information

16S sequences

  • @ref: 5303
  • description: Pseudomonas thermotolerans partial 16S rRNA gene, strain CM3
  • accession: AJ311980
  • length: 1429
  • database: ena
  • NCBI tax ID: 157784

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Pseudomonas thermotolerans DSM 14292GCA_000364625scaffoldncbi1123021
66792Pseudomonas thermotolerans DSM 142921123021.3wgspatric1123021
66792Pseudomonas thermotolerans DSM 142922521172647draftimg1123021

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileyes88.932no
flagellatedyes81.696no
gram-positiveno99.01no
anaerobicno98.409yes
aerobicyes90.582no
halophileno79.934no
spore-formingno96.553no
glucose-utilno53.514yes
thermophileno98.322no
glucose-fermentno92.433no

External links

@ref: 5303

culture collection no.: CCUG 54623, DSM 14292, KCTC 12117, LMG 21284, CIP 107795

straininfo link

  • @ref: 82285
  • straininfo: 85579

literature

  • topic: Phylogeny
  • Pubmed-ID: 12508889
  • title: Pseudomonas thermotolerans sp. nov., a thermotolerant species of the genus Pseudomonas sensu stricto.
  • authors: Manaia CM, Moore ERB
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/00207713-52-6-2203
  • year: 2002
  • mesh: Base Composition, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Fatty Acids/analysis, Hot Temperature, Lipids/analysis, Molecular Sequence Data, Phenotype, Phylogeny, Pseudomonas/*classification/genetics/isolation & purification/metabolism, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics
  • topic2: Stress

Reference

@idauthorscataloguedoi/urltitle
5303Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14292)https://www.dsmz.de/collection/catalogue/details/culture/DSM-14292
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
40715Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5269
60265Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 54623)https://www.ccug.se/strain?id=54623
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
82285Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID85579.1StrainInfo: A central database for resolving microbial strain identifiers
123518Curators of the CIPCollection of Institut Pasteur (CIP 107795)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107795