Strain identifier

BacDive ID: 130730

Type strain: Yes

Species: Streptomyces alkaliphilus

Strain Designation: No.7

Strain history: <- J. K. Akhwale, Jomo Kenyatta Univ. of Agriculture and Technol., Nairobi, Kenya; No.7

NCBI tax ID(s): 1472722 (species)

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General

@ref: 21963

BacDive-ID: 130730

DSM-Number: 42118

keywords: genome sequence, 16S sequence, Bacteria, mesophilic

description: Streptomyces alkaliphilus No.7 is a mesophilic bacterium that builds an aerial mycelium and was isolated from sediment from an alkaline, saline lake.

NCBI tax id

  • NCBI tax id: 1472722
  • Matching level: species

strain history

  • @ref: 21963
  • history: <- J. K. Akhwale, Jomo Kenyatta Univ. of Agriculture and Technol., Nairobi, Kenya; No.7

doi: 10.13145/bacdive130730.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces alkaliphilus
  • full scientific name: Streptomyces alkaliphilus Akhwale et al. 2015

@ref: 21963

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces alkaliphilus

full scientific name: Streptomyces alkaliphilus Akhwale et al. 2015

strain designation: No.7

type strain: yes

Morphology

colony morphology

@refcolony colormedium used
69346Grey beige (1019)ISP 4
69346ColourlessISP 6
69346ColourlessISP 7
69346ColourlessISP 2
69346Ivory (1014)ISP 3
69346Traffic yellow (1023)ISP 5

multicellular morphology

@refforms multicellular complexcomplex namemedium namecomplex color
69346noAerial myceliumISP 2
69346noAerial myceliumISP 3
69346yesAerial myceliumISP 4Cream (9001)
69346noAerial myceliumISP 5
69346noAerial myceliumISP 6
69346noAerial myceliumISP 7
69346yesAerial myceliumsuter with tyrosineIvory (1014), cream (9001)
69346yesAerial myceliumsuter without tyrosineIvory (1014), cream (9001)

pigmentation

@refproductionnamecolor
69346yesMelanin
69346yessoluble pigmentBrown beige (81011), black brown (8022)

multimedia

@refmultimedia contentcaptionintellectual property rights
21963https://www.dsmz.de/microorganisms/photos/DSM_42118.jpgMedium 1081 pH8 28°C© Leibniz-Institut DSMZ
66793EM_DSM_42118_1.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_42118_2.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_42118_3.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_42118_4.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_42118_5.jpgelectron microscopic image© HZI/Manfred Rohde
69346DSM_42118_image3.jpegPlates (65, ISP2, ISP3, ISP4, ISP5, ISP7)Helmholtz-Zentrum für Infektionsforschung GmbH
69346DSM_42118_image4.jpegPlates (65, ISP2, ISP3, ISP4, ISP5, ISP7)Helmholtz-Zentrum für Infektionsforschung GmbH

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
21963HORIKOSHI-1 MEDIUM (DSMZ Medium 1081)yeshttps://mediadive.dsmz.de/medium/1081Name: HORIKOSHI-1 MEDIUM (DSMZ Medium 1081) Composition: Agar 15.0 g/l Glucose 10.0 g/l Na2CO3 10.0 g/l Yeast extract 5.0 g/l Polypeptone 5.0 g/l K2HPO4 1.0 g/l MgSO4 x 7 H2O 0.2 g/l Distilled water
21963GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
21963STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)yeshttps://mediadive.dsmz.de/medium/252Name: STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) Composition: Agar 14.985 g/l Starch 9.99001 g/l (NH4)2SO4 1.998 g/l CaCO3 1.998 g/l K2HPO4 0.999001 g/l MgSO4 x 7 H2O 0.999001 g/l NaCl 0.999001 g/l FeSO4 x 7 H2O 0.000999001 g/l MnCl2 x 4 H2O 0.000999001 g/l ZnSO4 x 7 H2O 0.000999001 g/l Distilled water

culture temp

  • @ref: 21963
  • growth: positive
  • type: growth
  • temperature: 28
  • range: mesophilic

Physiology and metabolism

halophily

  • @ref: 69346
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-7.5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6934622599arabinose+growth
6934662968cellulose+/-growth
6934628757fructose+growth
6934617234glucose+growth
6934617268inositol-growth
6934637684mannose+growth
6934616634raffinose+/-growth
6934626546rhamnose+growth
6934617992sucrose+growth
6934618222xylose+growth
6837917632nitrate-reduction
683794853esculin-hydrolysis
6837916199urea-hydrolysis
683795291gelatin+hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

enzymes

@refvalueactivityec
68379gelatinase+
68379urease-3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase-3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase-3.5.1.B15
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382leucine arylamidase+3.4.11.1
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
69346---+------+--------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
69346+++/-+/-++/-+/---+/-+/-+/-+/--+----

Isolation, sampling and environmental information

isolation

  • @ref: 21963
  • sample type: sediment from an alkaline, saline lake
  • geographic location: Kenyan Rift Valley, Lake Elmenteita (0° 27'S, 36° 15' E) at 1776 m altitude
  • country: Kenya
  • origin.country: KEN
  • continent: Africa
  • latitude: -0.45
  • longitude: 36.25

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Lake (large)
#Environmental#Aquatic#Sediment
#Condition#Alkaline
#Condition#Saline

Safety information

risk assessment

  • @ref: 21963
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 21963
  • description: Streptomyces alkaliphilus strain No. 7 16S ribosomal RNA gene, partial sequence
  • accession: KF976730
  • length: 1466
  • database: ena
  • NCBI tax ID: 1472722

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptomyces alkaliphilus DSM 42118GCA_014141535contigncbi1472722
66792Streptomyces alkaliphilus strain DSM 421181472722.3wgspatric1472722

GC content

  • @ref: 21963
  • GC-content: 72.8
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno94.072no
gram-positiveyes93.074no
anaerobicno98.868no
aerobicyes91.767no
halophileno69.236no
spore-formingyes85.754no
thermophileno92.735yes
glucose-utilyes89.409yes
flagellatedno97.732no
glucose-fermentno87.764yes

External links

@ref: 21963

culture collection no.: DSM 42118, CECT 8549

straininfo link

  • @ref: 89233
  • straininfo: 406921

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny25761860Streptomyces alkaliphilus sp. nov., isolated from sediments of Lake Elmenteita in the Kenyan Rift Valley.Akhwale JK, Goker M, Rohde M, Sproer C, Schumann P, Klenk HP, Boga HIAntonie Van Leeuwenhoek10.1007/s10482-015-0418-22015Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry/metabolism, Geologic Sediments/*microbiology, Kenya, Lakes/*microbiology, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Streptomyces/*classification/genetics/*isolation & purification/metabolismMetabolism
Phylogeny33161356Streptomyces alkaliterrae sp. nov., isolated from an alkaline soil, and emended descriptions of Streptomyces alkaliphilus, Streptomyces calidiresistens and Streptomyces durbertensis.Swiecimska M, Golinska P, Nouioui I, Wypij M, Rai M, Sangal V, Goodfellow MSyst Appl Microbiol10.1016/j.syapm.2020.1261532020Alkalies, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Hydrogen-Ion Concentration, India, Lakes, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phosphatidylethanolamines/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Streptomyces/*classification/isolation & purification, Vitamin K 2/chemistryTranscriptome

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
21963Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-42118Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 42118)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
66793Mukherjee et al.GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life10.1038/nbt.3886 35: 676-683 201728604660
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69346Wink, J.Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweighttps://cdn.dsmz.de/wink/DSM%2042118.pdf
89233Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID406921.1