Strain identifier

BacDive ID: 130728

Type strain: Yes

Species: Streptomyces fractus

Strain Designation: MV32

Strain history: <- P. Meyers, Univ. Cape Town, South Africa; MV32 <- J. Rohland, Univ. Cape Town, South Africa

NCBI tax ID(s): 641806 (species)

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General

@ref: 21961

BacDive-ID: 130728

DSM-Number: 42163

keywords: 16S sequence, Bacteria, mesophilic

description: Streptomyces fractus MV32 is a mesophilic bacterium that was isolated from paunch region of the hindgut of the Black-mound Termite, Amitermes hastatus.

NCBI tax id

  • NCBI tax id: 641806
  • Matching level: species

strain history

  • @ref: 21961
  • history: <- P. Meyers, Univ. Cape Town, South Africa; MV32 <- J. Rohland, Univ. Cape Town, South Africa

doi: 10.13145/bacdive130728.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces fractus
  • full scientific name: Streptomyces fractus Rohland and Meyers 2016

@ref: 21961

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces fractus

full scientific name: Streptomyces fractus Rohland and Meyers 2016

strain designation: MV32

type strain: yes

Morphology

colony morphology

@refcolony colormedium used
69478ColourlessISP 7
69478Colourlesssuter with tyrosine
69478Colourlesssuter without tyrosine
69478ColourlessISP 2
69478ColourlessISP 6
69478Beige (1001)ISP 4
69478Ivory (1014)ISP 5
69478Light ivory (1015)ISP 3

multicellular morphology

@refforms multicellular complexcomplex namemedium name
69478noAerial myceliumISP 2
69478noAerial myceliumISP 3
69478noAerial myceliumISP 4
69478noAerial myceliumISP 5
69478noAerial myceliumISP 6
69478noAerial myceliumISP 7
69478noAerial myceliumsuter with tyrosine
69478noAerial myceliumsuter without tyrosine

pigmentation

@refproductionnamecolor
69478noMelanin
69478yessoluble pigmentSand yellow (1002)

multimedia

  • @ref: 21961
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_42163.jpg
  • caption: Medium 65 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
21961GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
21961ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://mediadive.dsmz.de/medium/84Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water
21961STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)yeshttps://mediadive.dsmz.de/medium/252Name: STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) Composition: Agar 14.985 g/l Starch 9.99001 g/l (NH4)2SO4 1.998 g/l CaCO3 1.998 g/l K2HPO4 0.999001 g/l MgSO4 x 7 H2O 0.999001 g/l NaCl 0.999001 g/l FeSO4 x 7 H2O 0.000999001 g/l MnCl2 x 4 H2O 0.000999001 g/l ZnSO4 x 7 H2O 0.000999001 g/l Distilled water

culture temp

  • @ref: 21961
  • growth: positive
  • type: growth
  • temperature: 28
  • range: mesophilic

Physiology and metabolism

halophily

  • @ref: 69478
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6947822599arabinose+growth
6947862968cellulose+/-growth
6947828757fructose+growth
6947817234glucose+growth
6947817268inositol+/-growth
6947837684mannose+growth
6947816634raffinose+growth
6947826546rhamnose+growth
6947817992sucrose+growth
6947818222xylose+growth
6837917632nitrate+reduction
683794853esculin+hydrolysis
6837916199urea+hydrolysis
683795291gelatin+hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

enzymes

@refvalueactivityec
68379gelatinase+
68379urease+3.5.1.5
68379beta-glucosidase+3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase+3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase-3.5.1.B15
68382alkaline phosphatase+3.1.3.1
68382esterase lipase (C 8)+
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase+3.2.1.24

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
69478+--+-++-+++--------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
69478++/-++/-+++/---++-+-+++++/-

Isolation, sampling and environmental information

isolation

  • @ref: 21961
  • sample type: paunch region of the hindgut of the Black-mound Termite, Amitermes hastatus
  • host species: Amitermes hastatus
  • geographic location: Cape Town, Tygerberg Nature Reserve
  • country: South Africa
  • origin.country: ZAF
  • continent: Africa

isolation source categories

Cat1Cat2Cat3
#Host#Arthropoda#Insecta
#Host Body-Site#Gastrointestinal tract

Safety information

risk assessment

  • @ref: 21961
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 21961
  • description: Streptomyces fractus strain MV32 16S ribosomal RNA gene, partial sequence
  • accession: FJ857947
  • length: 1474
  • database: ena
  • NCBI tax ID: 641806

External links

@ref: 21961

culture collection no.: DSM 42163, NRRL B-59159

straininfo link

  • @ref: 89232
  • straininfo: 400508

literature

  • topic: Phylogeny
  • Pubmed-ID: 25663029
  • title: Streptomyces fractus sp. nov., a novel streptomycete isolated from the gut of a South African termite.
  • authors: Rohland J, Meyers PR
  • journal: Antonie Van Leeuwenhoek
  • DOI: 10.1007/s10482-015-0404-8
  • year: 2015
  • mesh: Animals, Bacterial Typing Techniques, DNA, Bacterial/genetics, Gastrointestinal Tract/microbiology, Isoptera/*microbiology, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, South Africa, Streptomyces/*classification/genetics/*isolation & purification
  • topic2: Genetics

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
21961Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-42163Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 42163)
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69478Wink, J.Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweighttps://cdn.dsmz.de/wink/DSM%2042163.pdf
89232Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID400508.1