Strain identifier

BacDive ID: 130723

Type strain: Yes

Species: Streptomyces lunaelactis

Strain Designation: MM109

Strain history: <- S. Rigali, Université de Liége, Belgium; MM109 <- M. Maciejewska, Université of Liége,Belgium

NCBI tax ID(s): 1535768 (species)

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General

@ref: 21956

BacDive-ID: 130723

DSM-Number: 42149

keywords: genome sequence, 16S sequence, Bacteria, mesophilic

description: Streptomyces lunaelactis MM109 is a mesophilic bacterium that builds an aerial mycelium and was isolated from moonmilk speleothem from a cave.

NCBI tax id

  • NCBI tax id: 1535768
  • Matching level: species

strain history

  • @ref: 21956
  • history: <- S. Rigali, Université de Liége, Belgium; MM109 <- M. Maciejewska, Université of Liége,Belgium

doi: 10.13145/bacdive130723.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces lunaelactis
  • full scientific name: Streptomyces lunaelactis Maciejewska et al. 2015

@ref: 21956

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces lunaelactis

full scientific name: Streptomyces lunaelactis Maciejewska et al. 2015

strain designation: MM109

type strain: yes

Morphology

colony morphology

@refcolony colormedium used
69351Grey beige (1019)ISP 6
69351Green beige (1000)ISP 3
69351Ivory (1014)ISP 5
69351Ivory (1014), grey beige (1019)suter with tyrosine
69351Oyster white (1013)suter without tyrosine
69351Pale brown (8025)ISP 7
69351Reseda green (6011)ISP 4
69351Sand yellow (1002)ISP 2

multicellular morphology

@refforms multicellular complexcomplex namemedium namecomplex color
69351noAerial myceliumISP 2
69351yesAerial myceliumISP 3Pure white (9010)
69351yesAerial myceliumISP 4Traffic white (9016)
69351noAerial myceliumISP 5
69351noAerial myceliumISP 6
69351noAerial myceliumISP 7
69351noAerial myceliumsuter with tyrosine
69351noAerial myceliumsuter without tyrosine

pigmentation

@refproductionnamecolor
69351yesMelanin
69351yessoluble pigmentOlive brown (8008), grey beige (1019), black brown (8022)

multimedia

@refmultimedia contentcaptionintellectual property rights
21956https://www.dsmz.de/microorganisms/photos/DSM_42149.jpgMedium 65 28°C© Leibniz-Institut DSMZ
69351DSM_42149_image3.jpegPlates (65, ISP2, ISP3, ISP4, ISP5, ISP7)Helmholtz-Zentrum für Infektionsforschung GmbH
69351DSM_42149_image4.jpegPlates (65, ISP2, ISP3, ISP4, ISP5, ISP7)Helmholtz-Zentrum für Infektionsforschung GmbH

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
21956GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
21956STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)yeshttps://mediadive.dsmz.de/medium/252Name: STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) Composition: Agar 14.985 g/l Starch 9.99001 g/l (NH4)2SO4 1.998 g/l CaCO3 1.998 g/l K2HPO4 0.999001 g/l MgSO4 x 7 H2O 0.999001 g/l NaCl 0.999001 g/l FeSO4 x 7 H2O 0.000999001 g/l MnCl2 x 4 H2O 0.000999001 g/l ZnSO4 x 7 H2O 0.000999001 g/l Distilled water
21956ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://mediadive.dsmz.de/medium/84Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water

culture temp

  • @ref: 21956
  • growth: positive
  • type: growth
  • temperature: 28
  • range: mesophilic

Physiology and metabolism

compound production

  • @ref: 21956
  • compound: ferroverdin A

halophily

  • @ref: 69351
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6935122599arabinose-growth
6935162968cellulose+/-growth
6935128757fructose+growth
6935117234glucose+growth
6935117268inositol+/-growth
6935137684mannose+growth
6935116634raffinose+/-growth
6935126546rhamnose+growth
6935117992sucrose-growth
6935118222xylose+/-growth
6837917632nitrate+reduction
683794853esculin+hydrolysis
6837916199urea-hydrolysis
683795291gelatin+hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

enzymes

@refvalueactivityec
68379gelatinase+
68379urease-3.5.1.5
68379beta-glucosidase+3.2.1.21
68379N-acetyl-beta-glucosaminidase+3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase+3.4.19.3
68379pyrazinamidase-3.5.1.B15
68382alkaline phosphatase+3.1.3.1
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382trypsin+3.4.21.4
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase+3.2.1.24
68382alpha-fucosidase-3.2.1.51

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
69351+-++--+++-+--------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
69351++/-+/--+++/-++/-++-+/--++++-

Isolation, sampling and environmental information

isolation

  • @ref: 21956
  • sample type: moonmilk speleothem from a cave
  • geographic location: Comblain-au-Pont, 'Grotte des Collemboles'
  • country: Belgium
  • origin.country: BEL
  • continent: Europe

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Geologic

taxonmaps

  • @ref: 69479
  • File name: preview.99_135.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15;96_102;97_109;98_120;99_135&stattab=map
  • Last taxonomy: Streptomyces
  • 16S sequence: KM207217
  • Sequence Identity:
  • Total samples: 4030
  • soil counts: 2441
  • aquatic counts: 286
  • animal counts: 1009
  • plant counts: 294

Safety information

risk assessment

  • @ref: 21956
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
21956Streptomyces lunaelactis strain MM109 16S ribosomal RNA gene, partial sequenceKJ8627931399ena1535768
21956Streptomyces lunaelactis strain MM109 16S ribosomal RNA gene, complete sequenceKM2072171524ena1535768

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptomyces lunaelactis MM109GCA_003054555completencbi1535768
66792Streptomyces lunaelactis strain MM1091535768.3completepatric1535768
66792Streptomyces lunaelactis MM1092873250297completeimg1535768

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno98.074no
gram-positiveyes88.616no
anaerobicno99.437no
halophileno92.842no
spore-formingyes95.161no
glucose-utilyes90.57yes
aerobicyes92.015no
thermophileno98.751yes
motileno94.142no
glucose-fermentno88.161yes

External links

@ref: 21956

culture collection no.: DSM 42149, BCCM/LMG 28326, LMG 28326

straininfo link

  • @ref: 89229
  • straininfo: 399075

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny25491121Streptomyces lunaelactis sp. nov., a novel ferroverdin A-producing Streptomyces species isolated from a moonmilk speleothem.Maciejewska M, Pessi IS, Arguelles-Arias A, Noirfalise P, Luis G, Ongena M, Barton H, Carnol M, Rigali SAntonie Van Leeuwenhoek10.1007/s10482-014-0348-42014Bacterial Proteins/genetics, Bacterial Typing Techniques, Belgium, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, *Environmental Microbiology, Ferrous Compounds/*metabolism, Microscopy, Electron, Scanning, Molecular Sequence Data, Nitroso Compounds/*metabolism, Phylogeny, Pigments, Biological/analysis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Streptomyces/*classification/genetics/isolation & purification/*metabolismMetabolism
Genetics29798921Complete Genome Sequence of Streptomyces lunaelactis MM109(T), Isolated from Cave Moonmilk Deposits.Naome A, Maciejewska M, Calusinska M, Martinet L, Anderssen S, Adam D, Tenconi E, Deflandre B, Coppieters W, Karim L, Hanikenne M, Baurain D, Delfosse P, van Wezel GP, Rigali SGenome Announc10.1128/genomeA.00435-182018Phylogeny
Phylogeny32664387On the Risks of Phylogeny-Based Strain Prioritization for Drug Discovery: Streptomyces lunaelactis as a Case Study.Martinet L, Naome A, Baiwir D, De Pauw E, Mazzucchelli G, Rigali SBiomolecules10.3390/biom100710272020Aminobenzoates/*isolation & purification, Bacterial Proteins/*genetics, Biological Products/isolation & purification, Biosynthetic Pathways, Data Mining, Ferrous Compounds/*isolation & purification, Genome, Bacterial, Metabolomics, *Multigene Family, Nitroso Compounds/*isolation & purification, Phylogeny, Sequence Analysis, DNA, Streptomyces/chemistry/*classification/geneticsEnzymology

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
21956Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-42149Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 42149)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar SchomburgBRENDA, the ELIXIR core data resource in 2021: new developments and updates10.1093/nar/gkaa1025Nucleic Acids Res. 49: D498-D508 202033211880
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69351Wink, J.Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweighttps://cdn.dsmz.de/wink/DSM%2042149.pdf
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
89229Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID399075.1