Strain identifier
BacDive ID: 130720
Type strain: 
Species: Streptomyces leeuwenhoekii
Strain Designation: C34
Strain history: <- M. Goodfellow, Newcastle University; C34 <- C. K. Okoro
NCBI tax ID(s): 1437453 (species)
version 8.1 (current version)
General
@ref: 21953
BacDive-ID: 130720
DSM-Number: 42122
keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic, antibiotic compound production
description: Streptomyces leeuwenhoekii C34 is a spore-forming, mesophilic bacterium that produces antibiotic compounds and was isolated from hyper-arid desert soil.
NCBI tax id
- NCBI tax id: 1437453
- Matching level: species
strain history
- @ref: 21953
- history: <- M. Goodfellow, Newcastle University; C34 <- C. K. Okoro
doi: 10.13145/bacdive130720.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Kitasatosporales
- family: Streptomycetaceae
- genus: Streptomyces
- species: Streptomyces leeuwenhoekii
- full scientific name: Streptomyces leeuwenhoekii Busarakam et al. 2014
@ref: 21953
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Streptomycetaceae
genus: Streptomyces
species: Streptomyces leeuwenhoekii
full scientific name: Streptomyces leeuwenhoekii Busarakam et al. 2014
strain designation: C34
type strain: yes
Morphology
cell morphology
| @ref | motility | confidence | gram stain |
|---|---|---|---|
| 69480 | no | 94.028 | |
| 69480 | 100 | positive |
multimedia
- @ref: 21953
- multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_42122.jpg
- caption: Medium 65 28°C
- intellectual property rights: © Leibniz-Institut DSMZ
Culture and growth conditions
culture medium
- @ref: 21953
- name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)
- growth: yes
- link: https://mediadive.dsmz.de/medium/65
- composition: Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
culture temp
- @ref: 21953
- growth: positive
- type: growth
- temperature: 28
- range: mesophilic
Physiology and metabolism
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 69481 | yes | 100 |
| 69480 | yes | 100 |
compound production
| @ref | compound |
|---|---|
| 21953 | chaxalactins |
| 21953 | chaxamycins |
Isolation, sampling and environmental information
isolation
- @ref: 21953
- sample type: hyper-arid desert soil
- geographic location: Chaxa de Laguna, Salar de Atacama
- country: Chile
- origin.country: CHL
- continent: Middle and South America
- latitude: -23.2833
- longitude: -68.1667
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Terrestrial | #Desert |
| #Environmental | #Terrestrial | #Soil |
| #Climate | #Hot | #Arid |
Safety information
risk assessment
- @ref: 21953
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 21953
- description: Streptomyces leeuwenhoekii 16S ribosomal RNA gene, partial sequence
- accession: KF733382
- length: 1416
- database: ena
- NCBI tax ID: 1437453
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Streptomyces leeuwenhoekii type strain (C34 = DSM 42122 = NRRL B-24963) | GCA_001013905 | complete | ncbi | 1437453 |
| 66792 | Streptomyces leeuwenhoekii C34 | GCA_000696595 | scaffold | ncbi | 1437453 |
| 66792 | Streptomyces leeuwenhoekii strain C34 (= DSM 42122 = NRRL B-24963) strain C34 (= DSM 42122 = NRRL B-24963) strain type strain (C34 = DSM 42122 = NRRL B-24963) | 1437453.10 | plasmid | patric | 1437453 |
| 66792 | Streptomyces leeuwenhoekii strain C34 (= DSM 42122 = NRRL B-24963) strain C34 (= DSM 42122 = NRRL B-24963) strain type strain (C34 = DSM 42122 = NRRL B-24963) | 1437453.11 | plasmid | patric | 1437453 |
| 66792 | Streptomyces leeuwenhoekii strain type strain (C34 = DSM 42122 = NRRL B-24963) | 1437453.6 | complete | patric | 1437453 |
| 66792 | Streptomyces leeuwenhoekii DSM 42122 | 2872922060 | complete | img | 1437453 |
GC content
- @ref: 21953
- GC-content: 72.6
- method: high performance liquid chromatography (HPLC)
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | yes | 100 | no |
| motile | no | 93.776 | no |
| flagellated | no | 97.899 | no |
| gram-positive | yes | 91.098 | no |
| anaerobic | no | 99.39 | no |
| aerobic | yes | 93.87 | no |
| halophile | no | 91.883 | no |
| spore-forming | yes | 97.528 | no |
| thermophile | no | 94.991 | no |
| glucose-util | yes | 90.703 | no |
| glucose-ferment | no | 90.648 | no |
External links
@ref: 21953
culture collection no.: DSM 42122, NRRL B-24963
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 24604690 | Streptomyces leeuwenhoekii sp. nov., the producer of chaxalactins and chaxamycins, forms a distinct branch in Streptomyces gene trees. | Busarakam K, Bull AT, Girard G, Labeda DP, van Wezel GP, Goodfellow M | Antonie Van Leeuwenhoek | 10.1007/s10482-014-0139-y | 2014 | Anti-Bacterial Agents/*metabolism, Bacterial Typing Techniques, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Desert Climate, Macrolides/*metabolism, Microscopy, Electron, Scanning, Molecular Sequence Data, Multilocus Sequence Typing, Phylogeny, RNA, Ribosomal, 16S/genetics, Rifamycins/*metabolism, Soil Microbiology, Streptomyces/*classification/genetics/isolation & purification/*metabolism | Metabolism |
| Phylogeny | 25391353 | Streptomyces alkalithermotolerans sp. nov., a novel alkaliphilic and thermotolerant actinomycete isolated from a soda lake. | Sultanpuram VR, Mothe T, Mohammed F | Antonie Van Leeuwenhoek | 10.1007/s10482-014-0332-z | 2014 | Bacterial Typing Techniques, Base Composition, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Hydrogen-Ion Concentration, India, Lakes/*microbiology, Microscopy, Electron, Scanning, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Streptomyces/*classification/genetics/*isolation & purification/physiology | Genetics |
| Metabolism | 33741615 | Deciphering a Cyclodipeptide Synthase Pathway Encoding Prenylated Indole Alkaloids in Streptomyces leeuwenhoekii. | Zhang Y, Yao T, Jiang Y, Li H, Yuan W, Li W | Appl Environ Microbiol | 10.1128/AEM.02525-20 | 2021 | Bacterial Proteins/*metabolism, Indole Alkaloids/*metabolism, Metabolic Networks and Pathways, Microorganisms, Genetically-Modified/metabolism, Peptide Synthases/*metabolism, Prenylation, Streptomyces/enzymology/genetics/*metabolism | Enzymology |
Reference
| @id | authors | title | doi/url | catalogue |
|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
| 21953 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-42122 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 42122) | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | Predictions based on the model GenomeNet Sporulation v. 1 | 10.21203/rs.3.rs-2527258/v1 |