Strain identifier

BacDive ID: 130659

Type strain: Yes

Species: Mucinivorans hirudinis

Strain Designation: M3

Strain history: <- J. Graf, Univ. Connecticut, Storrs CT, USA; M3 <- M. Maltz

NCBI tax ID(s): 1433126 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 22150

BacDive-ID: 130659

DSM-Number: 27344

keywords: Bacteria, anaerobe, mesophilic

description: Mucinivorans hirudinis M3 is an anaerobe, mesophilic bacterium that was isolated from digestrive tract of the medicinal leech Hirudo verbana.

NCBI tax id

  • NCBI tax id: 1433126
  • Matching level: species

strain history

  • @ref: 22150
  • history: <- J. Graf, Univ. Connecticut, Storrs CT, USA; M3 <- M. Maltz

doi: 10.13145/bacdive130659.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Bacteroidia
  • order: Bacteroidales
  • family: Rikenellaceae
  • genus: Mucinivorans
  • species: Mucinivorans hirudinis
  • full scientific name: Mucinivorans hirudinis Nelson et al. 2015

@ref: 22150

domain: Bacteria

phylum: Bacteroidetes

class: Bacteroidia

order: Bacteroidales

family: Rikenellaceae

genus: Mucinivorans

species: Mucinivorans hirudinis

full scientific name: Mucinivorans hirudinis Nelson et al. 2015

strain designation: M3

type strain: yes

Morphology

colony morphology

  • @ref: 22150
  • incubation period: 2-3 days

Culture and growth conditions

culture medium

  • @ref: 22150
  • name: MODIFIED EGGERTH-GAGNON MEDIUM (DSMZ Medium 1580)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/1580
  • composition: Name: MODIFIED EGGERTH-GAGNON MEDIUM (DSMZ Medium 1580) Composition: Agar 15.0 g/l Peptone 10.0 g/l Na2HPO4 x 2 H2O 4.0 g/l Mucin from porcine stomach Type III 2.0 g/l Sheep blood Distilled water

culture temp

  • @ref: 22150
  • growth: positive
  • type: growth
  • temperature: 25
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 22150
  • oxygen tolerance: anaerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838016199urea-hydrolysis
6838029016arginine-hydrolysis
6838016024D-mannose-fermentation
6838016634raffinose-fermentation
6838029985L-glutamate+degradation
6838017632nitrate-reduction
6838027897tryptophan-energy source

metabolite production

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68380serine arylamidase-
68380glutamyl-glutamate arylamidase+
68380histidine arylamidase-
68380glycin arylamidase+
68380alanine arylamidase+3.4.11.2
68380tyrosine arylamidase-
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase-3.4.11.1
68380phenylalanine arylamidase-
68380leucyl glycin arylamidase+3.4.11.1
68380proline-arylamidase-3.4.11.5
68380L-arginine arylamidase-
68380alkaline phosphatase+3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase+4.1.1.15
68380N-acetyl-beta-glucosaminidase+3.2.1.52
68380beta-glucuronidase+3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380alpha-glucosidase+3.2.1.20
68380beta-Galactosidase 6-phosphate+
68380beta-galactosidase+3.2.1.23
68380alpha-galactosidase+3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
22150--+++++/--++--+---+--+----++-+-

Isolation, sampling and environmental information

isolation

  • @ref: 22150
  • sample type: digestrive tract (crop) of the medicinal leech Hirudo verbana
  • host species: Hirudo verbana
  • country: USA
  • origin.country: USA
  • continent: North America

isolation source categories

Cat1Cat2Cat3
#Host#Invertebrates (Other)#Annelida
#Host Body-Site#Gastrointestinal tract

Safety information

risk assessment

  • @ref: 22150
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

GC content

  • @ref: 22150
  • GC-content: 44.9
  • method: sequence analysis

External links

@ref: 22150

culture collection no.: DSM 27344, ATCC BAA 2553

straininfo link

  • @ref: 89169
  • straininfo: 400198

literature

  • topic: Phylogeny
  • Pubmed-ID: 25563920
  • title: Mucinivorans hirudinis gen. nov., sp. nov., an anaerobic, mucin-degrading bacterium isolated from the digestive tract of the medicinal leech Hirudo verbana.
  • authors: Nelson MC, Bomar L, Maltz M, Graf J
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.000052
  • year: 2015
  • mesh: Animals, Bacterial Typing Techniques, Bacteroidetes/*classification/genetics/isolation & purification, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Gastrointestinal Tract/*microbiology, Hirudo medicinalis/*microbiology, Molecular Sequence Data, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
  • topic2: Genetics

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
22150Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-27344Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 27344)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar SchomburgBRENDA, the ELIXIR core data resource in 2021: new developments and updates10.1093/nar/gkaa1025Nucleic Acids Res. 49: D498-D508 202033211880
68380Automatically annotated from API rID32A
89169Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID400198.1