Strain identifier
BacDive ID: 130659
Type strain:
Species: Mucinivorans hirudinis
Strain Designation: M3
Strain history: <- J. Graf, Univ. Connecticut, Storrs CT, USA; M3 <- M. Maltz
NCBI tax ID(s): 1433126 (species)
General
@ref: 22150
BacDive-ID: 130659
DSM-Number: 27344
keywords: Bacteria, anaerobe, mesophilic
description: Mucinivorans hirudinis M3 is an anaerobe, mesophilic bacterium that was isolated from digestrive tract of the medicinal leech Hirudo verbana.
NCBI tax id
- NCBI tax id: 1433126
- Matching level: species
strain history
- @ref: 22150
- history: <- J. Graf, Univ. Connecticut, Storrs CT, USA; M3 <- M. Maltz
doi: 10.13145/bacdive130659.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacteroidota
- domain: Bacteria
- phylum: Bacteroidota
- class: Bacteroidia
- order: Bacteroidales
- family: Rikenellaceae
- genus: Mucinivorans
- species: Mucinivorans hirudinis
- full scientific name: Mucinivorans hirudinis Nelson et al. 2015
@ref: 22150
domain: Bacteria
phylum: Bacteroidetes
class: Bacteroidia
order: Bacteroidales
family: Rikenellaceae
genus: Mucinivorans
species: Mucinivorans hirudinis
full scientific name: Mucinivorans hirudinis Nelson et al. 2015
strain designation: M3
type strain: yes
Morphology
colony morphology
- @ref: 22150
- incubation period: 2-3 days
Culture and growth conditions
culture medium
- @ref: 22150
- name: MODIFIED EGGERTH-GAGNON MEDIUM (DSMZ Medium 1580)
- growth: yes
- link: https://mediadive.dsmz.de/medium/1580
- composition: Name: MODIFIED EGGERTH-GAGNON MEDIUM (DSMZ Medium 1580) Composition: Agar 15.0 g/l Peptone 10.0 g/l Na2HPO4 x 2 H2O 4.0 g/l Mucin from porcine stomach Type III 2.0 g/l Sheep blood Distilled water
culture temp
- @ref: 22150
- growth: positive
- type: growth
- temperature: 25
- range: mesophilic
Physiology and metabolism
oxygen tolerance
- @ref: 22150
- oxygen tolerance: anaerobe
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
68380 | 16199 | urea | - | hydrolysis |
68380 | 29016 | arginine | - | hydrolysis |
68380 | 16024 | D-mannose | - | fermentation |
68380 | 16634 | raffinose | - | fermentation |
68380 | 29985 | L-glutamate | + | degradation |
68380 | 17632 | nitrate | - | reduction |
68380 | 27897 | tryptophan | - | energy source |
metabolite production
- @ref: 68380
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
- @ref: 68380
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
@ref | value | activity | ec |
---|---|---|---|
68380 | serine arylamidase | - | |
68380 | glutamyl-glutamate arylamidase | + | |
68380 | histidine arylamidase | - | |
68380 | glycin arylamidase | + | |
68380 | alanine arylamidase | + | 3.4.11.2 |
68380 | tyrosine arylamidase | - | |
68380 | pyrrolidonyl arylamidase | - | 3.4.19.3 |
68380 | leucine arylamidase | - | 3.4.11.1 |
68380 | phenylalanine arylamidase | - | |
68380 | leucyl glycin arylamidase | + | 3.4.11.1 |
68380 | proline-arylamidase | - | 3.4.11.5 |
68380 | L-arginine arylamidase | - | |
68380 | alkaline phosphatase | + | 3.1.3.1 |
68380 | tryptophan deaminase | - | 4.1.99.1 |
68380 | alpha-fucosidase | - | 3.2.1.51 |
68380 | glutamate decarboxylase | + | 4.1.1.15 |
68380 | N-acetyl-beta-glucosaminidase | + | 3.2.1.52 |
68380 | beta-glucuronidase | + | 3.2.1.31 |
68380 | alpha-arabinosidase | - | 3.2.1.55 |
68380 | alpha-glucosidase | + | 3.2.1.20 |
68380 | beta-Galactosidase 6-phosphate | + | |
68380 | beta-galactosidase | + | 3.2.1.23 |
68380 | alpha-galactosidase | + | 3.2.1.22 |
68380 | arginine dihydrolase | - | 3.5.3.6 |
68380 | urease | - | 3.5.1.5 |
API rID32A
@ref | URE | ADH Arg | alpha GAL | beta GAL | beta GP | alpha GLU | beta GLU | alpha ARA | beta GUR | beta NAG | MNE | RAF | GDC | alpha FUC | NIT | IND | PAL | ArgA | ProA | LGA | PheA | LeuA | PyrA | TyrA | AlaA | GlyA | HisA | GGA | SerA |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
22150 | - | - | + | + | + | + | +/- | - | + | + | - | - | + | - | - | - | + | - | - | + | - | - | - | - | + | + | - | + | - |
Isolation, sampling and environmental information
isolation
- @ref: 22150
- sample type: digestrive tract (crop) of the medicinal leech Hirudo verbana
- host species: Hirudo verbana
- country: USA
- origin.country: USA
- continent: North America
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Host | #Invertebrates (Other) | #Annelida |
#Host Body-Site | #Gastrointestinal tract |
Safety information
risk assessment
- @ref: 22150
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
GC content
- @ref: 22150
- GC-content: 44.9
- method: sequence analysis
External links
@ref: 22150
culture collection no.: DSM 27344, ATCC BAA 2553
straininfo link
- @ref: 89169
- straininfo: 400198
literature
- topic: Phylogeny
- Pubmed-ID: 25563920
- title: Mucinivorans hirudinis gen. nov., sp. nov., an anaerobic, mucin-degrading bacterium isolated from the digestive tract of the medicinal leech Hirudo verbana.
- authors: Nelson MC, Bomar L, Maltz M, Graf J
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.000052
- year: 2015
- mesh: Animals, Bacterial Typing Techniques, Bacteroidetes/*classification/genetics/isolation & purification, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Gastrointestinal Tract/*microbiology, Hirudo medicinalis/*microbiology, Molecular Sequence Data, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
- topic2: Genetics
Reference
@id | authors | title | doi/url | catalogue | journal | pubmed |
---|---|---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |||
22150 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-27344 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 27344) | |||
66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | 10.1093/nar/gkaa1025 | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | |
68380 | Automatically annotated from API rID32A | |||||
89169 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID400198.1 |