Strain identifier
BacDive ID: 130649
Type strain: 
Species: Azoarcus olearius
Strain Designation: DQS-4
Strain history: <- KCTC <- M.-H. Chen, Department of Seafood Science, University Kaohsing City; DQS-4
NCBI tax ID(s): 418699 (species)
version 8.1 (current version)
General
@ref: 21488
BacDive-ID: 130649
DSM-Number: 100663
keywords: genome sequence, 16S sequence, Bacteria, mesophilic, motile
description: Azoarcus olearius DQS-4 is a mesophilic, motile bacterium that was isolated from oil-contaminated soil.
NCBI tax id
- NCBI tax id: 418699
- Matching level: species
strain history
- @ref: 21488
- history: <- KCTC <- M.-H. Chen, Department of Seafood Science, University Kaohsing City; DQS-4
doi: 10.13145/bacdive130649.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Betaproteobacteria
- order: Rhodocyclales
- family: Zoogloeaceae
- genus: Azoarcus
- species: Azoarcus olearius
- full scientific name: Azoarcus olearius Chen et al. 2013
@ref: 21488
domain: Bacteria
phylum: Proteobacteria
class: Betaproteobacteria
order: Rhizobiales
family: Zoogloeaceae
genus: Azoarcus
species: Azoarcus olearius
full scientific name: Azoarcus olearius Chen et al. 2013
strain designation: DQS-4
type strain: yes
Morphology
cell morphology
| @ref | motility | confidence | gram stain |
|---|---|---|---|
| 69480 | yes | 98.466 | |
| 69480 | 99.999 | negative |
Culture and growth conditions
culture medium
- @ref: 21488
- name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)
- growth: yes
- link: https://mediadive.dsmz.de/medium/535
- composition: Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
culture temp
- @ref: 21488
- growth: positive
- type: growth
- temperature: 37
- range: mesophilic
Physiology and metabolism
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 69481 | no | 100 |
| 69480 | no | 99.96 |
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 68369 | 25115 | malate | + | assimilation |
| 68369 | 17128 | adipate | - | assimilation |
| 68369 | 27689 | decanoate | - | assimilation |
| 68369 | 24265 | gluconate | - | assimilation |
| 68369 | 17306 | maltose | - | assimilation |
| 68369 | 59640 | N-acetylglucosamine | - | assimilation |
| 68369 | 16899 | D-mannitol | - | assimilation |
| 68369 | 16024 | D-mannose | - | assimilation |
| 68369 | 30849 | L-arabinose | - | assimilation |
| 68369 | 17634 | D-glucose | - | assimilation |
| 68369 | 5291 | gelatin | - | hydrolysis |
| 68369 | 29016 | arginine | - | hydrolysis |
| 68369 | 17634 | D-glucose | - | fermentation |
| 68369 | 27897 | tryptophan | - | energy source |
| 68369 | 17632 | nitrate | + | reduction |
metabolite production
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 68369 | cytochrome oxidase | + | 1.9.3.1 |
| 68369 | gelatinase | - | |
| 68369 | arginine dihydrolase | - | 3.5.3.6 |
API 20NE
| @ref | NO3 | TRP | GLU_ Ferm | ADH Arg | URE | ESC | GEL | PNPG | GLU_ Assim | ARA | MNE | MAN | NAG | MAL | GNT | CAP | ADI | MLT | CIT | PAC | OX |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 21488 | + | - | - | - | + | + | - | - | - | - | - | - | - | - | - | - | - | + | - | + | + |
| 21488 | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | + | - | + | + |
Isolation, sampling and environmental information
isolation
- @ref: 21488
- sample type: oil-contaminated soil
- geographic location: Kaoshiung City, Chinese Petroleum Corporation refinery
- country: Taiwan
- origin.country: TWN
- continent: Asia
- latitude: 22.7217
- longitude: 120.296
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Engineered | #Contamination | #Oil (Fuel) |
| #Environmental | #Terrestrial | #Soil |
taxonmaps
- @ref: 69479
- File name: preview.99_3351.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_129;96_1751;97_2093;98_2570;99_3351&stattab=map
- Last taxonomy: Azoarcus olearius subclade
- 16S sequence: EF158388
- Sequence Identity:
- Total samples: 2059
- soil counts: 619
- aquatic counts: 798
- animal counts: 216
- plant counts: 426
Safety information
risk assessment
- @ref: 21488
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 21488
- description: Azoarcus olearius strain DQS-4 16S ribosomal RNA gene, partial sequence
- accession: EF158388
- length: 1456
- database: ena
- NCBI tax ID: 418699
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Azoarcus olearius DQS-4 | GCA_001682385 | complete | ncbi | 418699 |
| 66792 | Azoarcus olearius strain DQS4 | 418699.6 | complete | patric | 418699 |
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | no | 100 | no |
| flagellated | yes | 89.925 | no |
| gram-positive | no | 99.303 | no |
| anaerobic | no | 94.349 | no |
| aerobic | yes | 59.351 | no |
| halophile | no | 96.184 | no |
| spore-forming | no | 94.814 | no |
| thermophile | no | 97.614 | yes |
| glucose-util | no | 78.202 | yes |
| motile | yes | 90.968 | no |
| glucose-ferment | no | 88.407 | no |
External links
@ref: 21488
culture collection no.: DSM 100663, KCTC 23918, LMG 26893
straininfo link
- @ref: 89160
- straininfo: 379390
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 23645022 | Azoarcus olearius sp. nov., a nitrogen-fixing bacterium isolated from oil-contaminated soil. | Chen MH, Sheu SY, James EK, Young CC, Chen WM | Int J Syst Evol Microbiol | 10.1099/ijs.0.050609-0 | 2013 | Azoarcus/*classification/genetics/isolation & purification/metabolism, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/analysis, Hydroxybutyrates/analysis, Molecular Sequence Data, Nitrogen/metabolism, *Nitrogen Fixation, Nucleic Acid Hybridization, *Petroleum Pollution, Phospholipids/analysis, *Phylogeny, Polyesters/analysis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Taiwan | Metabolism |
| Metabolism | 27893193 | The oil-contaminated soil diazotroph Azoarcus olearius DQS-4(T) is genetically and phenotypically similar to the model grass endophyte Azoarcus sp. BH72. | Faoro H, Rene Menegazzo R, Battistoni F, Gyaneshwar P, do Amaral FP, Taule C, Rausch S, Goncalves Galvao P, de Los Santos C, Mitra S, Heijo G, Sheu SY, Chen WM, Mareque C, Zibetti Tadra-Sfeir M, Ivo Baldani J, Maluk M, Paula Guimaraes A, Stacey G, de Souza EM, Pedrosa FO, Magalhaes Cruz L, James EK | Environ Microbiol Rep | 10.1111/1758-2229.12502 | 2017 | Azoarcus/*genetics/*metabolism, Base Sequence, Endophytes/*genetics/metabolism, Gene Expression Regulation, Bacterial, Genome, Bacterial/*genetics, Iron/metabolism, Nitrogen Fixation/physiology, Oryza/*growth & development/microbiology, Sequence Analysis, DNA, Setaria Plant/*growth & development/microbiology, Soil Microbiology, Sulfur/metabolism | Genetics |
| Phylogeny | 32945762 | Azoarcus halotolerans sp. nov., a novel member of Rhodocyclaceae isolated from activated sludge collected in Hong Kong. | Li S, Zhao L, Han J, Liu S, Dai J, Fu G, Qiu D | Int J Syst Evol Microbiol | 10.1099/ijsem.0.004476 | 2020 | Azoarcus/*classification/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Hong Kong, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sewage/*microbiology | Transcriptome |
| Metabolism | 33220977 | Two-stage enrichment of hydrogen-oxidizing bacteria as biofertilizers. | Zhang W, Li YX, Niu Y, Zhang F, Li YB, Zeng RJ | Chemosphere | 10.1016/j.chemosphere.2020.128932 | 2020 | Azoarcus/metabolism, *Bacteria/metabolism, Carbon-Carbon Lyases/metabolism, *Hydrogen, Oxidation-Reduction |
Reference
| @id | authors | title | doi/url | catalogue |
|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
| 21488 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-100663 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 100663) | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
| 68369 | Automatically annotated from API 20NE | |||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | |
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | Predictions based on the model GenomeNet Sporulation v. 1 | 10.21203/rs.3.rs-2527258/v1 | |
| 89160 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID379390.1 |